Reviewed, UniProtKB/Swiss-Prot Q0SAY4 (SYP2_RHOSR)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase 2
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase 2
      Short name=ProRS 2

Gene names

Name:	proS2
Ordered Locus Names:	RHA1_ro03499

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Prolyl-tRNA synthetase 2 HAMAP MF_01571		PRO_0000288413

Sequences

	Sequence		Length	Mass (Da)
	Q0SAY4-1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 1D058198BB29ADE4	FASTA	479	52,973
	10 20 30 40 50 60 MARDERGVTA QSEDFAAWYN EVVFKAGLVD RGPAKGTMVI RPYGYRLWEL LQSELDRRIK 70 80 90 100 110 120 DTGHENAYFP LLIPESYLGR EAEHVEGFSP ELAVVTHVGG KVLEEPLVVR PTSETIIGEM 130 140 150 160 170 180 MAKWISSHRD LPLLLNQWAN VVRWELRPRM FLRTTEFLWQ EGHTAHVDEA SARRETMLAL 190 200 210 220 230 240 DIYHEVAREL AAIPVVPGEK TPGERFAGAV ATYTIEGMMR DGRALQSGTS HYMGIKFASA 250 260 270 280 290 300 FDIRFTSETG REELCHTTSW GMSTRMIGGI VMTHGDDKGL VFPPRLAPHQ VVIVPITRGG 310 320 330 340 350 360 NVAVEGAADE LAHRLRSVGV RTHVDARPHL TPGFKYNEWE MRGVPVRLEL GPRDLEDGTV 370 380 390 400 410 420 MMVKRLGDDG KQAVPIDSLP EAMPGVLDDF QAFLLARATA FRDGHARTVD NWTDFADAVS 430 440 450 460 470 TGWALALHCG IAACEEEIKS LTAATPRCVP LGGEPETGVC VRCGAASAYG KRVIFGRAY

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000431 Genomic DNA. Translation: ABG95302.1. Different initiation.
RefSeq	YP_703460.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4221050.
GenomeReviews	Gene locus RHA1_ro03499 in contig CP000431_GR.
KEGG	rha:RHA1_ro03499.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SAY4.
OMA	Q0SAY4. CIEAMMQ.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO03499-MON.
Family and domain databases
HAMAP	MF_01571. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP2_RHOSR

Accession

Primary (citable) accession number: Q0SAY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)