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Protein

Flavin-dependent monooxygenase, oxygenase subunit HsaA

Gene

hsaA

Organism
Rhodococcus jostii (strain RHA1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol.1 Publication

Catalytic activityi

3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH2 + O2 = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H2O.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei72 – 721FMNBy similarity
Binding sitei251 – 2511FMNBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi106 – 1083FMNBy similarity
Nucleotide bindingi129 – 1313FMNBy similarity
Nucleotide bindingi334 – 3352FMNBy similarity
Nucleotide bindingi356 – 3572FMNBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16961.
RJOS101510:GJJ1-4536-MONOMER.
UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavin-dependent monooxygenase, oxygenase subunit HsaA (EC:1.14.14.12)
Alternative name(s):
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4-hydroxylase, oxygenase subunit
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
Gene namesi
Name:hsaA
Ordered Locus Names:RHA1_ro04539
OrganismiRhodococcus jostii (strain RHA1)
Taxonomic identifieri101510 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
Proteomesi
  • UP000008710 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Flavin-dependent monooxygenase, oxygenase subunit HsaAPRO_0000404502Add
BLAST

Proteomic databases

PRIDEiQ0S811.

Interactioni

Subunit structurei

Homotetramer. HsaAB monooxygenase consists of an oxygenase component HsaA and a reductase component HsaB.1 Publication

Protein-protein interaction databases

STRINGi101510.RHA1_ro04539.

Structurei

Secondary structure

382
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 1313Combined sources
Helixi15 – 217Combined sources
Helixi26 – 3510Combined sources
Helixi37 – 393Combined sources
Helixi44 – 463Combined sources
Helixi53 – 6412Combined sources
Helixi68 – 8417Combined sources
Helixi89 – 968Combined sources
Beta strandi104 – 1074Combined sources
Beta strandi111 – 1177Combined sources
Beta strandi120 – 13011Combined sources
Helixi133 – 1353Combined sources
Beta strandi137 – 14711Combined sources
Beta strandi150 – 16011Combined sources
Helixi161 – 1633Combined sources
Beta strandi165 – 1673Combined sources
Beta strandi172 – 1743Combined sources
Helixi176 – 1783Combined sources
Beta strandi181 – 19111Combined sources
Helixi192 – 1943Combined sources
Beta strandi195 – 1973Combined sources
Helixi198 – 2025Combined sources
Helixi207 – 2093Combined sources
Helixi215 – 2184Combined sources
Helixi221 – 25434Combined sources
Helixi260 – 2623Combined sources
Helixi265 – 29733Combined sources
Helixi304 – 33229Combined sources
Helixi333 – 3375Combined sources
Helixi342 – 35312Combined sources
Helixi357 – 3593Combined sources
Helixi361 – 37212Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RFQX-ray1.65A/B/C/D1-382[»]
ProteinModelPortaliQ0S811.
SMRiQ0S811. Positions 1-382.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ0S811.

Family & Domainsi

Sequence similaritiesi

Belongs to the HpaH/HsaA monooxygenase family.Curated

Phylogenomic databases

eggNOGiENOG4108KC1. Bacteria.
ENOG410XS7W. LUCA.
HOGENOMiHOG000052204.
KOiK16047.
OMAiMAYGAYD.
OrthoDBiPOG091H08Q6.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR013107. Acyl-CoA_DH_C_dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF08028. Acyl-CoA_dh_2. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0S811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRLDALLPT LRERAQETED LRRIPDDSMK ALQETGFFRL LQPEQWGGYQ
60 70 80 90 100
ADPVLFYSAV RKIASACGST GWVSSIIGVH NWHLALFSQQ AQEDVWGNDT
110 120 130 140 150
DVRISSSYAP MGAGQVVDGG YTVNGAWAWS SGCDHASWAV LGGPVIKDGR
160 170 180 190 200
PVDFVSFLIP REDYRIDDVW NVVGLRGTGS NTVVVEDVFV PTHRVLSFKA
210 220 230 240 250
MSNLTAPGLE RNTAPVYKMP WGTIHPTTIS APIVGMAYGA YDAHVEHQGK
260 270 280 290 300
RVRAAFAGEK AKDDPFAKVR IAEASSDIDA AWRQLSGNVA DEYALLVAGE
310 320 330 340 350
EVPFELRLRA RRDQVRATGR AISSIDKLFE SSGATALANG TPLQRFWRDA
360 370 380
HAGRVHAAND PERAYVMYGT GEFGLPITDT MV
Length:382
Mass (Da):41,868
Last modified:September 5, 2006 - v1
Checksum:i18FA78081331E1AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA. Translation: ABG96325.1.

Genome annotation databases

EnsemblBacteriaiABG96325; ABG96325; RHA1_ro04539.
KEGGirha:RHA1_ro04539.
PATRICi23207981. VBIRhoJos26306_4575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA. Translation: ABG96325.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RFQX-ray1.65A/B/C/D1-382[»]
ProteinModelPortaliQ0S811.
SMRiQ0S811. Positions 1-382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi101510.RHA1_ro04539.

Proteomic databases

PRIDEiQ0S811.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG96325; ABG96325; RHA1_ro04539.
KEGGirha:RHA1_ro04539.
PATRICi23207981. VBIRhoJos26306_4575.

Phylogenomic databases

eggNOGiENOG4108KC1. Bacteria.
ENOG410XS7W. LUCA.
HOGENOMiHOG000052204.
KOiK16047.
OMAiMAYGAYD.
OrthoDBiPOG091H08Q6.

Enzyme and pathway databases

UniPathwayiUPA00062.
BioCyciMetaCyc:MONOMER-16961.
RJOS101510:GJJ1-4536-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ0S811.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR013107. Acyl-CoA_DH_C_dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF08028. Acyl-CoA_dh_2. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHSAA_RHOJR
AccessioniPrimary (citable) accession number: Q0S811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: September 5, 2006
Last modified: September 7, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.