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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Rhodococcus jostii (strain RHA1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901S-adenosyl-L-methionineUniRule annotation
Binding sitei115 – 1151S-adenosyl-L-methionineUniRule annotation
Binding sitei142 – 1421S-adenosyl-L-methionineUniRule annotation
Active sitei165 – 1651By similarity
Binding sitei165 – 1651S-adenosyl-L-methionineUniRule annotation
Binding sitei169 – 1691SubstrateUniRule annotation
Binding sitei201 – 2011SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRJOS101510:GJJ1-5173-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:RHA1_ro05176
OrganismiRhodococcus jostii (strain RHA1)
Taxonomic identifieri101510 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000008710 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258tRNA (guanine-N(7)-)-methyltransferasePRO_0000288211Add
BLAST

Proteomic databases

PRIDEiQ0S679.

Interactioni

Protein-protein interaction databases

STRINGi101510.RHA1_ro05176.

Structurei

3D structure databases

ProteinModelPortaliQ0S679.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni235 – 2384Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0S679-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPETPLMRDN GPVNHADQDA PAVPEEGQTK DSKGSRLHPR VTSFRSRRGA
60 70 80 90 100
LTVAQQESWD RQWPRIGADV SDHRLDAPSW FGRDAPLILE IGSGTGTATA
110 120 130 140 150
AMAKAEPHVN LMAVEVYRPG LAQLLQQIER DEIPNIRVLR GDAMDVLENM
160 170 180 190 200
IEPESLTGVR VFFPDPWPKA RHHKRRLLQS PTFALIASRL KAGGVLHVAT
210 220 230 240 250
DHAEYAEAIA EAGNAEPLLT SLDWESPMTH ERPVTKFEDK AHQVGSAITE

LIWGKIRS
Length:258
Mass (Da):28,586
Last modified:September 5, 2006 - v1
Checksum:iCD758B30746B1F02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA. Translation: ABG96957.1.
RefSeqiWP_011597391.1. NC_008268.1.
YP_705115.1. NC_008268.1.

Genome annotation databases

EnsemblBacteriaiABG96957; ABG96957; RHA1_ro05176.
KEGGirha:RHA1_ro05176.
PATRICi23209297. VBIRhoJos26306_5228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA. Translation: ABG96957.1.
RefSeqiWP_011597391.1. NC_008268.1.
YP_705115.1. NC_008268.1.

3D structure databases

ProteinModelPortaliQ0S679.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi101510.RHA1_ro05176.

Proteomic databases

PRIDEiQ0S679.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG96957; ABG96957; RHA1_ro05176.
KEGGirha:RHA1_ro05176.
PATRICi23209297. VBIRhoJos26306_5228.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciRJOS101510:GJJ1-5173-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RHA1.

Entry informationi

Entry nameiTRMB_RHOJR
AccessioniPrimary (citable) accession number: Q0S679
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: September 5, 2006
Last modified: May 27, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.