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Protein

Malate dehydrogenase

Gene

mdh

Organism
Rhodococcus jostii (strain RHA1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951SubstrateUniRule annotation
Binding sitei101 – 1011SubstrateUniRule annotation
Binding sitei108 – 1081NADUniRule annotation
Binding sitei115 – 1151NADUniRule annotation
Binding sitei134 – 1341SubstrateUniRule annotation
Binding sitei165 – 1651SubstrateUniRule annotation
Active sitei190 – 1901Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 207NADUniRule annotation
Nucleotide bindingi132 – 1343NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciRJOS101510:GJJ1-6241-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:RHA1_ro06244
OrganismiRhodococcus jostii (strain RHA1)
Taxonomic identifieri101510 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus
ProteomesiUP000008710 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Malate dehydrogenasePRO_0000292374Add
BLAST

Proteomic databases

PRIDEiQ0S365.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi101510.RHA1_ro06244.

Structurei

3D structure databases

ProteinModelPortaliQ0S365.
SMRiQ0S365. Positions 7-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFKDTDY.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0S365-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQSPPPAVV TVTGAAGSIG YASLFRIAAG EMLGPDTPIR LRLLEIPSAV
60 70 80 90 100
SAAEGTAMEL DDSAFPLLRD IEVHDDPKRG FDGTDVALLI GSRPRSKGME
110 120 130 140 150
RGDLLAANGQ IFTVQGRALN QVAADGVRVL VVGNPANTNA LVAANNAPDI
160 170 180 190 200
PAERFTALTR LDHNRAIAQL ARHSGAAVKD IRRVAIWGNH SSTQYPDIFH
210 220 230 240 250
ARVGDRSGAE FAADREWLTG DFIPTVANRG SAIIEARGAS SAASAANAAI
260 270 280 290 300
DHVHDWVLGT PDDDWTSVAL PSTGAYGVPE GLVSSFPVRS VDGSWQIVDG
310 320 330
LEIDDFSRKL IDASVGELES ERDAVRGMGF I
Length:331
Mass (Da):34,864
Last modified:September 5, 2006 - v1
Checksum:iF0354F0E927A296B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA. Translation: ABG98021.1.
RefSeqiWP_011598209.1. NC_008268.1.
YP_706179.1. NC_008268.1.

Genome annotation databases

EnsemblBacteriaiABG98021; ABG98021; RHA1_ro06244.
GeneIDi4223795.
KEGGirha:RHA1_ro06244.
PATRICi23211433. VBIRhoJos26306_6292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000431 Genomic DNA. Translation: ABG98021.1.
RefSeqiWP_011598209.1. NC_008268.1.
YP_706179.1. NC_008268.1.

3D structure databases

ProteinModelPortaliQ0S365.
SMRiQ0S365. Positions 7-331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi101510.RHA1_ro06244.

Proteomic databases

PRIDEiQ0S365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG98021; ABG98021; RHA1_ro06244.
GeneIDi4223795.
KEGGirha:RHA1_ro06244.
PATRICi23211433. VBIRhoJos26306_6292.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFKDTDY.
OrthoDBiEOG6PP9Q2.

Enzyme and pathway databases

BioCyciRJOS101510:GJJ1-6241-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RHA1.

Entry informationi

Entry nameiMDH_RHOJR
AccessioniPrimary (citable) accession number: Q0S365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: September 5, 2006
Last modified: June 24, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.