Q0S2X1 (AROA_RHOSR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	RHA1_ro06338

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000325378

Sequences

	Sequence		Length	Mass (Da)
	Q0S2X1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 01626E54D413E88B	FASTA	438	45,110
	10 20 30 40 50 60 METVSLSLWN APRAEAPVVA TVALPGSKSI TNRALILAAL ADGPSTLTGA LRSRDTDLMI 70 80 90 100 110 120 EALRTLGISI ETVGADTTLR VTPGPLQGGA VDCGLAGTVM RFLPPVAALA SGTVHFDGDE 130 140 150 160 170 180 QARTRPLDTI LDALRGLGAD IDGASLPFTV RGAGSLRGGR VTIDASGSSQ FVSGLLLSAA 190 200 210 220 230 240 AFDEGVTVHH DGKTVPSMPH IDMTVEMLRE SGVEVTTPAT GGEADTWRVS PGVVRAVDRA 250 260 270 280 290 300 IEPDLSNATA FLAAAAVTGG EVTVPLWPSR TTQPGDAIRE ILLAMGADVR LDGANLTVRG 310 320 330 340 350 360 PQQLTGIDID LHDVGELTPT VAALAALADG PSHLRGIAHL RGHETDRLAA LAHEINSLGG 370 380 390 400 410 420 NVTETEDGLT IVPAGLHGGT WRSYADHRMA TAGAIVGLRV DGIRIEDVGT TAKTLPGFEN 430 LWATMLSAAA GTERKASF

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RHA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG98115.1.
RefSeq	YP_706273.1. NC_008268.1.
3D structure databases
ProteinModelPortal	Q0S2X1.
SMR	Q0S2X1. Positions 8-425.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0S2X1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4223889.
GenomeReviews	Gene locus RHA1_ro06338 in contig CP000431_GR.
KEGG	rha:RHA1_ro06338.
PATRIC	23211627. VBIRhoJos26306_6389.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HBG646626.
OMA	HRMATAG.
PhylomeDB	Q0S2X1.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO06338-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_RHOSR

Accession

Primary (citable) accession number: Q0S2X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program