Q0S2B9 (RNH2_RHOSR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	RHA1_ro06544

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Ribonuclease HII HAMAP MF_00052_B		PRO_0000334944
Sites
	Metal binding	35	1	Divalent metal cation By similarity
	Metal binding	36	1	Divalent metal cation By similarity
	Metal binding	129	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0S2B9 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 6234C24308974650	FASTA	226	23,808
	10 20 30 40 50 60 MTSWPPRIVI RRSSGLRTME SALVRSGLGP VAGVDEAGRG ACAGPLVVAA CVLAPKPYPA 70 80 90 100 110 120 LARLDDSKKL TERTREELFP AITRLAVAWS VVSFPAEEVD RMGVHVANIE GMRRAVAGLS 130 140 150 160 170 180 TTPGYVLTDG FRVPGLPAPS LPVIGGDAAA ACIAAASVLA KVSRDRVMVA MDETHPGYGF 190 200 210 220 AIHKGYNTPA HLTALEKLGP CPEHRRSWSN VAALIHRVDN SSGSAR

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RHA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG98317.1.
RefSeq	YP_706475.1. NC_008268.1.
3D structure databases
ProteinModelPortal	Q0S2B9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0S2B9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4224095.
GenomeReviews	Gene locus RHA1_ro06544 in contig CP000431_GR.
KEGG	rha:RHA1_ro06544.
PATRIC	23212055. VBIRhoJos26306_6599.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	Q0S2B9.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO06544-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_RHOSR

Accession

Primary (citable) accession number: Q0S2B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program