Q0S1I5 (Q0S1I5_RHOSR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116
Short name=dUTPase HAMAP MF_00116
EC=3.6.1.23 HAMAP MF_00116

Alternative name(s):
dUTP pyrophosphatase HAMAP MF_00116

Gene names

Name:	dut HAMAP MF_00116
Ordered Locus Names:	RHA1_ro06829

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP] EMBL ABG98601.1

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family. HAMAP MF_00116

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_00116
Ligand	Magnesium HAMAP MF_00116 Metal-binding HAMAP MF_00116
Molecular function	Hydrolase HAMAP MF_00116 EMBL ABG98601.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	78 – 80	3	Substrate binding By similarity HAMAP MF_00116
	Region	95 – 97	3	Substrate binding By similarity HAMAP MF_00116
Sites
	Binding site	91	1	Substrate By similarity HAMAP MF_00116
	Binding site	105	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity HAMAP MF_00116

Sequences

	Sequence		Length	Mass (Da)
	Q0S1I5 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: D87E8B97DBC88746	FASTA	171	17,457
	10 20 30 40 50 60 MSDLSPGLSP AATPALPPVA LQRLDPDIPV PQRAHDGDAG VDLCSTVDVT IEPGRRVLVG 70 80 90 100 110 120 TGIAIALPLG TVGLIHPRSG LAAKSGLSVV NTPGTIDAGY RGEIKVCLIN HDLETPIEIR 130 140 150 160 170 RGDRIAQLLV QRVELVSFVE VPSLDGTTRG SDGHGSSGGH ASLVGNASEG A

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG98601.1.
RefSeq	YP_706759.1. NC_008268.1.
3D structure databases
ProteinModelPortal	Q0S1I5.
SMR	Q0S1I5. Positions 7-158.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0S1I5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4224380.
GenomeReviews	Gene locus RHA1_ro06829 in contig CP000431_GR.
KEGG	rha:RHA1_ro06829.
PATRIC	23212643. VBIRhoJos26306_6892.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HBG436079.
OMA	KIAQMVI.
PhylomeDB	Q0S1I5.
ProtClustDB	PRK00601.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO06829-MONOMER.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q0S1I5_RHOSR

Accession

Primary (citable) accession number: Q0S1I5

Entry history

Integrated into UniProtKB/TrEMBL:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)