Q0S1H1 (DXS_RHOSR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	RHA1_ro06843

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256477

Sequences

	Sequence		Length	Mass (Da)
	Q0S1H1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 3DD1E66326E673C2	FASTA	649	68,492
	10 20 30 40 50 60 MGVLARIQTP DDLRQLNSAE MKQLAAEIRE FLVQKVAATG GHLGPNLGVV ELTLALHRIF 70 80 90 100 110 120 DSPADPIIFD TGHQAYVHKI LTGRKDDFDS LRKQGGLSGY PCRAESDHDW VESSHASASL 130 140 150 160 170 180 SYADGLAKAF ELTGQDRHVV AVVGDGALTG GMCWEALNNI AAGKDRSVVI VVNDNGRSYA 190 200 210 220 230 240 PTIGGLADHL AALRLQPGYE RILDSGRRMV KKLPWVGRTA YSVLHGMKAG LKDAVAPQVM 250 260 270 280 290 300 FTDLGIKYLG PVDGHDEAAL ESALRRAKAF GGPVIVHAVT RKGMGYAPAE NHVADQMHST 310 320 330 340 350 360 GVIDPITGRG TGSASADWTS VFSAELIDQA SHRQDIVAIT AAMAGPTGLA AFGEKYPDRM 370 380 390 400 410 420 FDVGIAEQHA VTSAAGLALG GLHPVVAVYS TFLNRAFDQL LMDVALLKLP VTLVLDRAGI 430 440 450 460 470 480 TGNDGASHNG MWDMSLLGIV PGMKVAAPRD TATLREELDE ALAVDDGPTA LRFPKGTVGD 490 500 510 520 530 540 DVPAVSRLDG VVDILRAPSG RNDVLIVSVG AFAGLALAAA ERLEQQGISA TVVDPRWVLP 550 560 570 580 590 600 VPESLVKLAE DYTMVVTVED SGLHGGVGST VSAALRAAGV DVPCRDLGVP QQFLDHASRA 610 620 630 640 QIHAELGLTA QDVARQITGW FAGLGNLRPE QQNGVVADLD AQRAEKQQG

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RHA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG98615.1.
RefSeq	YP_706773.1. NC_008268.1.
3D structure databases
ProteinModelPortal	Q0S1H1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0S1H1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4224394.
GenomeReviews	Gene locus RHA1_ro06843 in contig CP000431_GR.
KEGG	rha:RHA1_ro06843.
PATRIC	23212673. VBIRhoJos26306_6907.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	QYDISYM.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO06843-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_RHOSR

Accession

Primary (citable) accession number: Q0S1H1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program