Q0S0M4 (PYRB_RHOSR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	RHA1_ro07148

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000301611

Sequences

	Sequence		Length	Mass (Da)
	Q0S0M4 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 49D412A6766C4158	FASTA	314	33,675
	10 20 30 40 50 60 MKHLLSIADL TRESAVELLD EAERFEQALL GREVRKLPTL RGRTVMTVFF ENSTRTRVSF 70 80 90 100 110 120 EVAGKWMSAD VINVSASSSS VSKGESLRDT AMTLRAAGAD ALIVRHPASG AAHQIAKWTG 130 140 150 160 170 180 HQDDGGPAVI NAGDGTHEHP TQALLDALTL RQRLGDIEGK RIAIVGDILH SRVARSNALL 190 200 210 220 230 240 LSMLGAEVVL VAPPTLLPVG VSAWPVSVAH SLDAELPGLD AVLMLRVQAE RMNGGFFPSQ 250 260 270 280 290 300 REYSINYGLS EKRLALLPDH AVVLHPGPML RGMEIASAVA DSTRTAVLQQ VTNGVHMRMA 310 VLFRLLVGAE EVAG

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RHA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG98912.1.
RefSeq	YP_707070.1. NC_008268.1.
3D structure databases
ProteinModelPortal	Q0S0M4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0S0M4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4224698.
GenomeReviews	Gene locus RHA1_ro07148 in contig CP000431_GR.
KEGG	rha:RHA1_ro07148.
PATRIC	23213273. VBIRhoJos26306_7201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0540.
HOGENOM	HBG579429.
OMA	LQRERMS.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO07148-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_RHOSR

Accession

Primary (citable) accession number: Q0S0M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program