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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Frankia alni (strain ACN14a)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21D-inositol 3-phosphateUniRule annotation1
Binding sitei16UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei711D-inositol 3-phosphateUniRule annotation1
Binding sitei1041D-inositol 3-phosphateUniRule annotation1
Binding sitei1281D-inositol 3-phosphateUniRule annotation1
Binding sitei1481D-inositol 3-phosphateUniRule annotation1
Binding sitei222UDP-GlcNAcUniRule annotation1
Binding sitei227UDP-GlcNAcUniRule annotation1
Metal bindingi297Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi298Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi300Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei310UDP-GlcNAcUniRule annotation1
Binding sitei318UDP-GlcNAcUniRule annotation1
Metal bindingi324MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:FRAAL0950
OrganismiFrankia alni (strain ACN14a)
Taxonomic identifieri326424 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000000657 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001221 – 407D-inositol 3-phosphate glycosyltransferaseAdd BLAST407

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi326424.FRAAL0950.

Structurei

3D structure databases

ProteinModelPortaliQ0RS49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 9UDP-GlcNAc bindingUniRule annotation2
Regioni13 – 181D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0RS49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTSPMEQPG TGDAGGLNVY VVELSRQLAA LGVEVEVFTR AVSSKLPTSA
60 70 80 90 100
ELAPGVTVRH VDAGPFEEIH REDLPAWLCA FTADVLRAEA GHEPGYFDVI
110 120 130 140 150
HSHYWLSGQV ALAVARRWGV PFVHTSHTLA KIKNGALAVG DRPEPPGRLL
160 170 180 190 200
GEQEVIAGST RLIASTADER GHLIDLYDAD PDRVDVVAPG VDLETFRPGD
210 220 230 240 250
PAQSRARLGL DRDGDLLLFV GRIQPLKAPD LLLHAAAELL RRDPTRRSRL
260 270 280 290 300
TVAVVGGPSG SGLEQPDALV KLAADLGISD LVRFQPPAPQ HELAHWYRAA
310 320 330 340 350
TAVVVPSHSE SFGLVALEAQ ACGTPVVAAA VGGLRTAVAD GVSGLLVAGR
360 370 380 390 400
DPARYADTLD RLLRQPHWRS RLAAGAVGRA AGFGWSATAR GVLRSYRHAL

SPAAVAV
Length:407
Mass (Da):43,127
Last modified:September 5, 2006 - v1
Checksum:i0E9C86B93DA94669
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573213 Genomic DNA. Translation: CAJ59616.1.

Genome annotation databases

KEGGifal:FRAAL0950.
PATRICi21911761. VBIFraAln347_0848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573213 Genomic DNA. Translation: CAJ59616.1.

3D structure databases

ProteinModelPortaliQ0RS49.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326424.FRAAL0950.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGifal:FRAAL0950.
PATRICi21911761. VBIFraAln347_0848.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_FRAAA
AccessioniPrimary (citable) accession number: Q0RS49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: September 5, 2006
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.