Q0RNP9 (RSMH_FRAAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	FRAAL2188

Organism

Frankia alni (strain ACN14a) [Complete proteome] [HAMAP]

Taxonomic identifier

326424 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000386903
Regions
	Region	33 – 35	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	52	1	S-adenosyl-L-methionine By similarity
	Binding site	87	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	101	1	S-adenosyl-L-methionine By similarity
	Binding site	108	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0RNP9 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: C4BD8BA23EE57E30	FASTA	325	34,785
	10 20 30 40 50 60 MHTPVLTDRV LELLAPALSR PGAVVVDATV GLGGHAAALL AAFPEVRVVG LDRDLDALAH 70 80 90 100 110 120 SGERLRALAP ERVRLVHAVY DEIGTVLAGL GSARVQGVLF DLGVSSLQLD TDARGFAYSR 130 140 150 160 170 180 DAPLDMRMDA TRGLTAAEVL NTYDAAALAR ILRDYGEERF ARRIAESVVR ARAVEPFTTS 190 200 210 220 230 240 ARLVDLVRDA IPAPARRTGG NPAKRTFQAL RIEVNSELDV LARALPAAFD ALAVGGRLVV 250 260 270 280 290 300 LSYHSLEDRL VKRELRGYAE TLVPPDMPVV PAGAEPRLRW LTRGAEPAGE VEKADNPRAA 310 320 SVRLRAAERT APNPDRTQPT IGGAS

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ACN14a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT573213 Genomic DNA. Translation: CAJ60837.1.
RefSeq	YP_712416.1. NC_008278.1.
3D structure databases
ProteinModelPortal	Q0RNP9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0RNP9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4232213.
GenomeReviews	Gene locus FRAAL2188 in contig CT573213_GR.
KEGG	fal:FRAAL2188.
PATRIC	21914088. VBIFraAln347_2001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0275.
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	FALN326424:FRAAL2188-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_FRAAA

Accession

Primary (citable) accession number: Q0RNP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program