Q0RFA3 (Q0RFA3_FRAAA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281
EC=6.1.1.20 HAMAP MF_00281

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281

Gene names

Name:	pheS HAMAP MF_00281 EMBL CAJ63844.1
Ordered Locus Names:	FRAAL5211

Organism

Frankia alni (strain ACN14a) [Complete proteome] [HAMAP] EMBL CAJ63844.1

Taxonomic identifier

326424 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity. HAMAP MF_00281
Subcellular location	Cytoplasm By similarity HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily. HAMAP MF_00281

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00281
Cellular component	Cytoplasm HAMAP MF_00281
Ligand	ATP-binding HAMAP MF_00281 Magnesium HAMAP MF_00281 Metal-binding HAMAP MF_00281 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00281 EMBL CAJ63844.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q0RFA3 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: F8875AA1F11D84FC	FASTA	367	40,034
	10 20 30 40 50 60 MPAPGETVDP SRVHALDPDQ LAAAVAAARE SIRAAADLDA LAAARTAHVH GAAAPLVLAR 70 80 90 100 110 120 RELGALPREE RADAGRRHNA ARAEVQAAYD TRLAELTAER DARVLREERV DVTLPVARRR 130 140 150 160 170 180 RGARHPLSSL SDRLVDVFVA MGYEVAEGPE IEHDWFNFEA LNMAPDHPAR SEHDTLYVEP 190 200 210 220 230 240 TASGRVLRTH TSPVQVRALL ERPLPVYVVC PGRTYRNDTI DATHSPVFGQ LEGLAVDEGI 250 260 270 280 290 300 TMADLRGTLE VFAQALFGSG LSTRLRPSFF PFTEPSAELD VQCFACRGEA TRQPCRVCSD 310 320 330 340 350 360 EGWIEIGGCG MVDPNVLRAT GVDPTRYSGF AFGMGLERAA MIRHGVREIR DFVDGDVRFG LPFGIEV

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT573213 Genomic DNA. Translation: CAJ63844.1.
RefSeq	YP_715378.1. NC_008278.1.
3D structure databases
ProteinModelPortal	Q0RFA3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0RFA3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4236971.
GenomeReviews	Gene locus FRAAL5211 in contig CT573213_GR.
KEGG	fal:FRAAL5211.
PATRIC	21919640. VBIFraAln347_4771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q0RFA3_FRAAA

Accession

Primary (citable) accession number: Q0RFA3

Entry history

Integrated into UniProtKB/TrEMBL:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)