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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Frankia alni (strain ACN14a)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi103ZincUniRule annotation1
Metal bindingi105ZincUniRule annotation1
Metal bindingi125ZincUniRule annotation1
Metal bindingi127ZincUniRule annotation1
Binding sitei239ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFALN326424:FRAAL_RS22945-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:FRAAL5260
OrganismiFrankia alni (strain ACN14a)
Taxonomic identifieri326424 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia
Proteomesi
  • UP000000657 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000018991 – 470Glutamate--tRNA ligaseAdd BLAST470

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi326424.FRAAL5260

Structurei

3D structure databases

ProteinModelPortaliQ0RF57
SMRiQ0RF57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionUniRule annotationAdd BLAST11
Motifi236 – 240"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiRNIEGKD

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q0RF57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGRVRVRF APSPTGIFHV GGARSALFNW LVALRAGGDF VLRVEDTDAS
60 70 80 90 100
RNRPEWTDGI ISALDWLGIS PGRYEGPVLQ SSRADRHRAA AVRLREAGLA
110 120 130 140 150
YFCDCTREAL AERTGNAQHG YDGFCRDRGL EPGPGRALRF RTPDDGVTTV
160 170 180 190 200
HDLIRGTPEF PNDTIEDFVI ARADGSAVFL LANVVDDLEM GITHVIRGEE
210 220 230 240 250
HLSNTPKQQL LWAALGAAEP PVWAHVPVIV NEKRQKLSKR RDKVALESYR
260 270 280 290 300
DEGYLPGAMK NYLMLLGWAP SGDDEIVPWE TIESTFDLAD VKPSPAFFDE
310 320 330 340 350
KKLRAFNGEY IRALSVEEFI AAVEPWLAPP AAPWAADAFD AGTFAAVAGL
360 370 380 390 400
AQSRVSVLAE IVPMVDFLFL PAAPVDDAAW AKAMKGPAAQ LLADVHDVFG
410 420 430 440 450
KIEWDAETLK ATLAELGERH GLKLAKAQAP VRVAVTGRTV GLPLFESLEV
460 470
FGREATRRRI AAARDRLAAG
Length:470
Mass (Da):51,235
Last modified:September 5, 2006 - v1
Checksum:iA3B2118E7A675150
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573213 Genomic DNA Translation: CAJ63893.1
RefSeqiWP_011606351.1, NC_008278.1

Genome annotation databases

KEGGifal:FRAAL5260

Similar proteinsi

Entry informationi

Entry nameiSYE_FRAAA
AccessioniPrimary (citable) accession number: Q0RF57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: June 20, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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