Reviewed, UniProtKB/Swiss-Prot Q0REL2 (PANC2_FRAAA)

Names and origin

Protein names

Recommended name:
    Pantothenate synthetase 2
      Short name=PS 2
    EC=6.3.2.1
Alternative name(s):
    Pantoate--beta-alanine ligase 2
    Pantoate-activating enzyme 2

Gene names

Name:	panC2
Ordered Locus Names:	FRAAL5463

Organism

Frankia alni (strain ACN14a) [Complete proteome] [HAMAP]

Taxonomic identifier

326424 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity.
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; pantothenate biosynthesis; pantothenate from beta-alanine and pantoate: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity.
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Pantothenate synthetase 2 HAMAP MF_00158		PRO_0000305454
Regions
	Nucleotide binding	167 – 170	4	ATP By similarity
	Nucleotide binding	204 – 207	4	ATP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	196	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0REL2-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 0782103C69ED6697	FASTA	330	34,091
	10 20 30 40 50 60 MSGNGGGIGT GLGSLAGSGR TVTTIAEVRA AADAVRAAGG RVGFFGTSGA LHEGHLTVIR 70 80 90 100 110 120 RMAAECDLSI MPLFLAPVPG VTSDAVPAYD RDFDADAALA FDAGVNLVFR PAVAEMYPAL 130 140 150 160 170 180 PVRTAVVPAD ELAAPWEGAE DPSFLRMAAT ALAKYYNIVG PCRAYTGEKD WVPLTVLRRM 190 200 210 220 230 240 VVDLSIRAEI VACPVVRLAD GLCASSRNSR LSAADRAAAP TLYAALTAAV AAVEAGERDA 250 260 270 280 290 300 EAIRSLLRRR ISAVAPVDYA EVVDATTLAR VDPLAGELRL LVSADFTGTH LFDNVGLTVR 310 320 330 DADADADARV TVPAVGTTAA APATDRRTSG

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CT573213 Genomic DNA. Translation: CAJ64096.1.
RefSeq	YP_715623.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4234010.
GenomeReviews	Gene locus FRAAL5463 in contig CT573213_GR.
KEGG	fal:FRAAL5463.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	FALN326424:FRAAL5463-MON.
Family and domain databases
HAMAP	MF_00158. [Tree]
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PANC2_FRAAA

Accession

Primary (citable) accession number: Q0REL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)