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Q0RD46 (BIOB_FRAAA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:FRAAL6003
OrganismFrankia alni (strain ACN14a) [Complete proteome] [HAMAP]
Taxonomic identifier326424 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351Biotin synthase HAMAP-Rule MF_01694
PRO_0000381386

Sites

Metal binding881Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding921Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding951Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1641Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2231Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2931Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0RD46 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: E6614AE0C7E8581F

FASTA35137,470
        10         20         30         40         50         60 
MTAHIAPATV PPSAPAAAGQ DPAALLAHAR REVLEAGRGL DEAGVLAVLR LPDEYLGDAL 

        70         80         90        100        110        120 
ALAHEVRMRW CGPEVEVEGI ISLKTGGCPE DCHFCSQSGK FDSPVRSAWL DVPSLVEAAR 

       130        140        150        160        170        180 
QTAATGATEF CIVAAVRGPD ARLMSQVREG VAAIRAAVDI NVACSLGMLT QDQVDELTAI 

       190        200        210        220        230        240 
GVHRYNHNLE TSRSHFPKVV TTHSWEERWE TCEMVRAAGM ELCCGAILGV GESLEQRAEL 

       250        260        270        280        290        300 
AAQLAVLEPD EVPLNFLNPR PGTPFGDYPP VQPRDALRAI AAFRLALPRT ILRYSGGREI 

       310        320        330        340        350 
TLGDLEAQGM LGGINAVIVG NYLTTLGRPA ESDLKMLADL SMPIKSLQAT L 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CT573213 Genomic DNA. Translation: CAJ64628.1.
RefSeqYP_716142.1. NC_008278.1.

3D structure databases

ProteinModelPortalQ0RD46.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326424.FRAAL6003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4235414.
KEGGfal:FRAAL6003.
PATRIC21921102. VBIFraAln347_5494.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.
ProtClustDBPRK06256.

Enzyme and pathway databases

BioCycFALN326424:GJ82-5900-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_FRAAA
AccessionPrimary (citable) accession number: Q0RD46
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 5, 2006
Last modified: February 19, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways