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Q0RD46

- BIOB_FRAAA

UniProt

Q0RD46 - BIOB_FRAAA

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Protein

Biotin synthase

Gene

bioB

Organism
Frankia alni (strain ACN14a)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi164 – 1641Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi223 – 2231Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi293 – 2931Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciFALN326424:GJ82-5900-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:FRAAL6003
OrganismiFrankia alni (strain ACN14a)
Taxonomic identifieri326424 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeFrankiaceaeFrankia
ProteomesiUP000000657: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Biotin synthasePRO_0000381386Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi326424.FRAAL6003.

Structurei

3D structure databases

ProteinModelPortaliQ0RD46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0RD46 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAHIAPATV PPSAPAAAGQ DPAALLAHAR REVLEAGRGL DEAGVLAVLR
60 70 80 90 100
LPDEYLGDAL ALAHEVRMRW CGPEVEVEGI ISLKTGGCPE DCHFCSQSGK
110 120 130 140 150
FDSPVRSAWL DVPSLVEAAR QTAATGATEF CIVAAVRGPD ARLMSQVREG
160 170 180 190 200
VAAIRAAVDI NVACSLGMLT QDQVDELTAI GVHRYNHNLE TSRSHFPKVV
210 220 230 240 250
TTHSWEERWE TCEMVRAAGM ELCCGAILGV GESLEQRAEL AAQLAVLEPD
260 270 280 290 300
EVPLNFLNPR PGTPFGDYPP VQPRDALRAI AAFRLALPRT ILRYSGGREI
310 320 330 340 350
TLGDLEAQGM LGGINAVIVG NYLTTLGRPA ESDLKMLADL SMPIKSLQAT

L
Length:351
Mass (Da):37,470
Last modified:September 5, 2006 - v1
Checksum:iE6614AE0C7E8581F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT573213 Genomic DNA. Translation: CAJ64628.1.
RefSeqiYP_716142.1. NC_008278.1.

Genome annotation databases

GeneIDi4235414.
KEGGifal:FRAAL6003.
PATRICi21921102. VBIFraAln347_5494.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT573213 Genomic DNA. Translation: CAJ64628.1 .
RefSeqi YP_716142.1. NC_008278.1.

3D structure databases

ProteinModelPortali Q0RD46.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326424.FRAAL6003.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4235414.
KEGGi fal:FRAAL6003.
PATRICi 21921102. VBIFraAln347_5494.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci FALN326424:GJ82-5900-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ACN14a.

Entry informationi

Entry nameiBIOB_FRAAA
AccessioniPrimary (citable) accession number: Q0RD46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 5, 2006
Last modified: October 1, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3