Reviewed, UniProtKB/Swiss-Prot Q0RC35 (PANC3_FRAAA)

Names and origin

Protein names

Recommended name:
    Pantothenate synthetase 3
      Short name=PS 3
    EC=6.3.2.1
Alternative name(s):
    Pantoate--beta-alanine ligase 3
    Pantoate-activating enzyme 3

Gene names

Name:	panC3
Ordered Locus Names:	FRAAL6371

Organism

Frankia alni (strain ACN14a) [Complete proteome] [HAMAP]

Taxonomic identifier

326424 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Pantothenate synthetase 3 HAMAP MF_00158		PRO_0000305455
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity
	Nucleotide binding	154 – 157	4	ATP By similarity
	Nucleotide binding	191 – 194	4	ATP By similarity
Sites
	Active site	38	1	Proton donor By similarity
	Binding site	62	1	Beta-alanine By similarity
	Binding site	62	1	Pantoate By similarity
	Binding site	160	1	Pantoate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0RC35-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: DF3A6AE9A22D1B1E	FASTA	294	32,511
	10 20 30 40 50 60 MLRVLHTIED VREQTREWRA AGSSIGCVMT MGALHEGHLS LVDAARRECD KVVLTLFVNP 70 80 90 100 110 120 IQFGPSEDFD RYPRTEEADF RALRDRECDA VFAPATETIF PLGERRIDQV RTKVVVRGLT 130 140 150 160 170 180 DVLCGPRRPG HFDGVTTEVL KMLHIVDCDR TYWGEKDYQQ YAVIRAMVED QGLPVKVVPC 190 200 210 220 230 240 PTMRDVDGLA LSSRNTYLDS GQRAIAPRLY AALRRGARRI AEQGIDVIGE TTKQIAETLL 250 260 270 280 290 AHGFDLVEYV EVYTGSLGPA VAGTPVEELR VFGAVRLGGA RLLDNAAVAD EVGG

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT573213 Genomic DNA. Translation: CAJ64994.1.
RefSeq	YP_716505.1.
3D structure databases
SMR	Q0RC35. Positions 2-288.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0RC35.
Genome annotation databases
GeneID	4233711.
GenomeReviews	Gene locus FRAAL6371 in contig CT573213_GR.
KEGG	fal:FRAAL6371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HBG428839.
OMA	SQFAPHE.
PhylomeDB	Q0RC35.
Enzyme and pathway databases
BioCyc	FALN326424:FRAAL6371-MONOMER.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC3_FRAAA

Accession

Primary (citable) accession number: Q0RC35

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	September 5, 2006
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)