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Q0RBI4 (Q0RBI4_FRAAA) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL CAJ65202.1
Ordered Locus Names:FRAAL6579
OrganismFrankia alni (strain ACN14a) [Complete proteome] [HAMAP] EMBL CAJ65202.1
Taxonomic identifier326424 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length1036 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q0RBI4 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 2C0D29FF5CCC5DEA

FASTA1,036110,198
        10         20         30         40         50         60 
MPATRPSSRA AAGRAATGRR PTSRSATGSG GSTGAAAEPT PGSGTRLVIV ESPAKAKTIA 

        70         80         90        100        110        120 
GYLGPGWQVE SSIGHIRDLP RSAADVPAAH KGKPWARLGV DVDNDFEPLY VVTPDKKLQV 

       130        140        150        160        170        180 
SKLKALVKEA SELYLATDED REGEAIAWHL LQTLKPTVPV KRMVFHEITP QAIRRAVDNP 

       190        200        210        220        230        240 
REIDENLVNA QETRRILDRL YGYEVSPVLW KKVMPKLSAG RVQSVATRVL VERERARMRF 

       250        260        270        280        290        300 
RAAEYWNIEG LFGATVARQA WPAGTDGADA DATGGGVERT PLPATLVALD GNRIATGRDF 

       310        320        330        340        350        360 
APTGELTTSG VTRLDEAGAR ALTDRLAQAA FAVRSVETKP YRRSPYPPFM TSTLQQEAGR 

       370        380        390        400        410        420 
KLRFSSQRTM QIAQRLYENG YITYMRTDST NLSETALAAA RSQAESLYGP EYVPASPRTY 

       430        440        450        460        470        480 
TKKVKNAQEA HEAIRPAGDH FRTPGEVRGE LDVDSYRLYE LIWQRTVASQ MADARGTSAT 

       490        500        510        520        530        540 
IRLGATSSTG EDAEFSASGK VITFPGFLRA YVEGADDPDA ELEDRERRLP DVRQGDPLAV 

       550        560        570        580        590        600 
RSLTPRGHTT SPPARFTEAS LVKTLEELGI GRPSTYASII GTIQDRGYVW KKGSALVPSF 

       610        620        630        640        650        660 
VAFAVVGLLE DHFGRLVDYR FTATMEDDLD DIAAGTSAST DWLTRFYFGT GDGTDPATAA 

       670        680        690        700        710        720 
GLKHLVSERL GEIDAREVNS IPLGKADDGT TVVVRVGRYG PYVQLGEGRA SVPDDLAPDE 

       730        740        750        760        770        780 
LTVARALELL AAPSGDRVLG VDAATGATIT AKAGRFGPYV TTDTDPPRTS SLLRTMSLET 

       790        800        810        820        830        840 
LTLDDAVKLL TLPRTLGAGA DGEEVTAQNG RYGPYVKKGA ESRSLESEDQ LFTVTLEEAL 

       850        860        870        880        890        900 
ALLAQPKARG RRQAAQTPPL RELGNDPASG KPMVVREGRF GPYVTDGETN ASLRKGDAVE 

       910        920        930        940        950        960 
SITDARAAEL LADRRARGPA PAKRAARGTA KTTATKTTAT KTTAAKSSAA KTTTAKSTTA 

       970        980        990       1000       1010       1020 
KTGAAKSTTA KTAAKSATTK SATTRGAAAK SATTRSGTKS STAKKVGEES AATGSSAPDD 

      1030 
GGGAAEAASG GTRRTD

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CT573213 Genomic DNA. Translation: CAJ65202.1.
RefSeqYP_716706.1. NC_008278.1.

3D structure databases

ProteinModelPortalQ0RBI4.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0RBI4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4235440.
GenomeReviewsGene locus FRAAL6579 in contig CT573213_GR.
KEGGfal:FRAAL6579.
PATRIC21922148. VBIFraAln347_6012.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0550.
HOGENOMHBG512935.
OMAKKVMPRL.
PhylomeDBQ0RBI4.
ProtClustDBCLSK949032.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 3 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ0RBI4_FRAAA
AccessionPrimary (citable) accession number: Q0RBI4
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2006
Last sequence update: September 5, 2006
Last modified: December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info