Q0RAT5 (POTA_FRAAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine/putrescine import ATP-binding protein PotA
EC=3.6.3.31

Gene names

Name:	potA
Ordered Locus Names:	FRAAL6832

Organism

Frankia alni (strain ACN14a) [Complete proteome] [HAMAP]

Taxonomic identifier

326424 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia › Frankia alni

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system By similarity.
Catalytic activity	ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In).
Subunit structure	The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD) Potential.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	ATP-binding cassette (ABC) transporter complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW putrescine-importing ATPase activity Inferred from electronic annotation. Source: InterPro spermidine-importing ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Spermidine/putrescine import ATP-binding protein PotA		PRO_0000286219
Regions
	Domain	30 – 264	235	ABC transporter
	Nucleotide binding	66 – 73	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0RAT5 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: F740084088D540CB	FASTA	465	49,827
	10 20 30 40 50 60 MTATSGARTS DARTSGARTS DAGIGSPAAI ELVGVAKDYQ TRGRAVPAVS RVDLAIRPGE 70 80 90 100 110 120 FFSLLGPSGC GKSTTLRMIA GFEEPTRGRI LLQGRDVTAV PPNRRDVNLV FQSYALFPHL 130 140 150 160 170 180 DVAGNVAFGL RRRGVKGREL RERVGAMLDL VELSELADRR PRELSGGQQQ RVALARALVN 190 200 210 220 230 240 RPAALLLDEP LGALDLKLRQ TMQIQLKAIQ REVGITFLYV THDQGEALTM SDRIAVMNGG 250 260 270 280 290 300 RVEQLASPRD IYERPRTRFV AGFIGTSNLL RRTVRALDAA GPGGRPTVVL DAGTDERLSA 310 320 330 340 350 360 PLHPPTGPLR AGDQVELTVR PEKIEMSVSR PDGPGCALRG RIVEVVYLGT YTTYTVATHG 370 380 390 400 410 420 GTEITVFWQN STGAGNVAER GDEIWLSWLP EHSYVLPEPV ALPEPVALPG SVVPPETVNQ 430 440 450 460 PDSVGPPDSI IPPHSVVAPD SVDPRETVVP AAGTSADPAA YRISH

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ACN14a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT573213 Genomic DNA. Translation: CAJ65455.1.
RefSeq	YP_716959.1. NC_008278.1.
3D structure databases
ProteinModelPortal	Q0RAT5.
ModBase	Search...
Protein-protein interaction databases
STRING	326424.FRAAL6832.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4236983.
KEGG	fal:FRAAL6832.
PATRIC	21922617. VBIFraAln347_6241.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3842.
KO	K11072.
OMA	VPPNRRD.
ProtClustDB	CLSK938605.
Enzyme and pathway databases
BioCyc	FALN326424:GJ82-6731-MONOMER.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR017879. ABC_Sperm/Put_ATP-bd_PotA. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR008995. Mo/tungstate-bd_C_term_dom. IPR027417. P-loop_NTPase. IPR005893. Sp_pt_ABC_ATP-bd. IPR013611. Transp-assoc_OB_typ2. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. PF08402. TOBE_2. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50331. MOP_like. 1 hit. SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR01187. potA. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51305. POTA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POTA_FRAAA

Accession

Primary (citable) accession number: Q0RAT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	September 5, 2006
Last modified:	May 29, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program