Q0RAS3 (Q0RAS3_FRAAA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase HAMAP MF_00012
Short name=DAD HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD HAMAP MF_00012 EMBL CAJ65467.1
Ordered Locus Names:	FRAAL6844

Organism

Frankia alni (strain ACN14a) [Complete proteome] [HAMAP] EMBL CAJ65467.1

Taxonomic identifier

326424 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Frankiaceae › Frankia

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL CAJ65467.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	108	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	183	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	Q0RAS3 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 73E42EC57EA22902	FASTA	603	63,025
	10 20 30 40 50 60 MAGARALWRA TGMTDDDFGK PIIAIANSFT QFVPGHVHLR NLGALVAEAV AESGGVGREF 70 80 90 100 110 120 NTIAVDDGIA MGHGGMLYSL PSREIIADSV EYMVNAHCAD ALVCISNCDK ITPGMLLAAL 130 140 150 160 170 180 RLNIPTVFVS GGAMESGNAV ISGGTARSKL DLITAMSAAV NPEVSDGDLS TIERSACPTC 190 200 210 220 230 240 GSCSGMFTAN SMNCLTEAMG LSLPGNGSTL ATAAARRELF VEAGRLVVDL ARRYYEKDDE 250 260 270 280 290 300 AVLPRSIATA AAFRNAFAVD VAMGGSTNTV LHLLAAAVEA GVDVTLADID QISRTVPCLC 310 320 330 340 350 360 KVAPSSTHYY MEDVHRAGGI PAILGELDRA GLLDRNTHSV HAASLREFLD RWDVRGAQPS 370 380 390 400 410 420 PDAVELFHAA PGGVRTIEPF SSTNRWDSLD TDAENGCIRS VEHAYSAEGG LAVLFGNLAT 430 440 450 460 470 480 DGAVVKTAGV DESQWSFRGP ALVVESQEDA VDAILNKRVQ PGDVIIVRYE GPRGGPGMQE 490 500 510 520 530 540 MLYPTAFLKG RGLGPKCALI TDGRFSGGSS GLSIGHVSPE AAHGGTIALV RDGDVIDIDI 550 560 570 580 590 600 PTRRLELLVS DEELARRRAE LEAAGGYRPA NRERAVSAAL RAYAAMATSA STGAARDVSL LGG

References

[1]

"Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography." Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N., Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A., Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P., Goltsman E., Huang Y. , Kopp O.R., Labarre L., Lapidus A., Lavire C., Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J., Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F., Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C., Benson D.R. Genome Res. 17:7-15(2007) [PubMed: 17151343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT573213 Genomic DNA. Translation: CAJ65467.1.
RefSeq	YP_716971.1. NC_008278.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0RAS3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4234544.
GenomeReviews	Gene locus FRAAL6844 in contig CT573213_GR.
KEGG	fal:FRAAL6844.
PATRIC	21922641. VBIFraAln347_6253.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q0RAS3.
ProtClustDB	PRK12448.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q0RAS3_FRAAA

Accession

Primary (citable) accession number: Q0RAS3

Entry history

Integrated into UniProtKB/TrEMBL:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)