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Protein

Delphilin

Gene

Grid2ip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Postsynaptic scaffolding protein at the Purkinje cell synapse, where it may serve to link GRID2 with actin cytoskeleton and various signaling molecules.

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • G-protein coupled glutamate receptor signaling pathway Source: MGI
  • long term synaptic depression Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Delphilin
Alternative name(s):
Glutamate receptor, ionotropic, delta 2-interacting protein 1
Gene namesi
Name:Grid2ip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2176213. Grid2ip.

Subcellular locationi

Isoform 2 :
Isoform 3 :

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • dendritic spine Source: MGI
  • postsynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12031203DelphilinPRO_0000331625Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei303 – 3031PhosphoserineCombined sources
Modified residuei572 – 5721PhosphoserineCombined sources
Modified residuei613 – 6131PhosphoserineCombined sources
Modified residuei644 – 6441PhosphoserineCombined sources
Modified residuei647 – 6471PhosphoserineCombined sources
Isoform 2 (identifier: Q0QWG9-2)
Lipidationi3 – 31S-palmitoyl cysteine

Post-translational modificationi

Isoform 2 is palmitoylated. Palmitoylation of isoform 2 is necessary for the enhanced cell surface expression of GRID2, and is also responsible for the accumulation of isoform 2 within dendritic spines. Isoform 1 and isoform 2 are differentially localized, probably modulating GRID2 signaling in neurons.

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ0QWG9.
PaxDbiQ0QWG9.
PRIDEiQ0QWG9.

PTM databases

iPTMnetiQ0QWG9.
PhosphoSiteiQ0QWG9.
SwissPalmiQ0QWG9.

Expressioni

Tissue specificityi

Isoform 1 is expressed in the cerebellum, but not in the cerebral cortex. Isoform 2 is expressed in the cell body of purkinge cells of the cerebellum and weakly expressed in the cerebrum and the brainstem as well as various nuclei of the thalamus. Isoform 2 is highly expressed in the cerebral cortex than in the cerebellum. Isoform 3 is expressed in the cerebellum and cerebrum.2 Publications

Developmental stagei

Isoform 3 expression is maintained throughout cerebellar development, while isoform 2 expression gradually decrease following the first postnatal week.1 Publication

Gene expression databases

BgeeiQ0QWG9.
GenevisibleiQ0QWG9. MM.

Interactioni

Subunit structurei

Interacts with C-terminus of the glutamate receptor GRID2 via PDZ domain. Isoform 2 interacts also with Profilin-2/PFN2 and with the monocarboxylate transporter SLC16A7 via PDZ domain. The interaction of isoform 2 with GRID2 is dependent on GRID2 phosphorylation by PKA.6 Publications

Protein-protein interaction databases

BioGridi228467. 1 interaction.
STRINGi10090.ENSMUSP00000106361.

Structurei

3D structure databases

ProteinModelPortaliQ0QWG9.
SMRiQ0QWG9. Positions 26-57, 267-341, 821-1182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 7979PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini268 – 34578PDZ 2PROSITE-ProRule annotationAdd
BLAST
Domaini812 – 1203392FH2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi742 – 80463Pro-richAdd
BLAST

Domaini

PDZ 1 domain is responsible for cytoplasmic clustering of isoform 1.

Sequence similaritiesi

Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
KOG3528. Eukaryota.
ENOG410XSM6. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000112812.
HOVERGENiHBG107964.
InParanoidiQ0QWG9.
OMAiCQSMSPS.
OrthoDBiEOG7DJSKF.
PhylomeDBiQ0QWG9.
TreeFamiTF329416.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015425. FH2_Formin.
IPR001478. PDZ.
[Graphical view]
PfamiPF02181. FH2. 1 hit.
PF00595. PDZ. 2 hits.
[Graphical view]
SMARTiSM00498. FH2. 1 hit.
SM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS51444. FH2. 1 hit.
PS50106. PDZ. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q0QWG9-1) [UniParc]FASTAAdd to basket

Also known as: L-delphilin

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPATNQGWPE DFGFQLGGSG PCFVIEVAEG SSAHAGGLRP GDQILEVEGL
60 70 80 90 100
AVGGLSRERI VRLARRCPRV PPSLGVLPGP EGGPTALTAA WLTRRFGRSL
110 120 130 140 150
PLSRELLRLA GGPRPDAVHR ERRRKAQEFS CQVDDILGDR LTAKEQVFTA
160 170 180 190 200
LKQFAAEQRV DELVWTLTLV LPSEAQGPVL DNLRIFIPKK HRARFDEVVS
210 220 230 240 250
QGLLGKLCRA RRAQGAQRLR RSRSEERPER LLVSTRASAA PRRPDEPPPR
260 270 280 290 300
KATSLLGGRT GPGGPRRTVR VYKGNKSFGF TLRGHGPVWI ESVLPGSPAE
310 320 330 340 350
NASLKSGDRI LFLNGLDMRN CSHDKVVSML QGSGAMPTLV VEEGPVPFAS
360 370 380 390 400
DSDSLDSPTR ASALTSLQWV ADILPSSIRV QGRTFSQQLD HLLTPPERYG
410 420 430 440 450
VCRALERFFQ HRNIDTLIVD VYPVLDTPAK QVLWQFLYQL LTYEEQELCQ
460 470 480 490 500
EKIACFLGYT AMTEPESSLD LEPESTPEPT PEPQPRSSLR ASSMCRRSLR
510 520 530 540 550
SQGLETSLSC GPGDCPEMPL PLIPGERQAG DGTSLPETPN PKMMSAVYAE
560 570 580 590 600
LESRLNSSFK GKIGTMSKSR ASPPVPSLVG TSGPRTLSGV SWPSDRLLPS
610 620 630 640 650
PCYDPLCSGG LASPSSSESH PYASLDSSRA PSPQPGLGSI HADSPPSPDP
660 670 680 690 700
IRPPSRRKLF AFSRPVRSRD TDRFLDALSE QLGPRLSIVD DFLTPENDYE
710 720 730 740 750
EMSFHDDQGS FVTNERSSAS ECVSSSEEGS SLTYSSISDH IPPPPLSPPP
760 770 780 790 800
PPPLPFHDPK PSSRTSDGPR GPPQSLTKPL TQINHPVPPP PPPPLPPPVP
810 820 830 840 850
CAPPMLSRGV GHRRSETSHM SVKRLRWEQV ENSEGTIWGQ LGEDSDYDKL
860 870 880 890 900
SDMVKYLDLE LHFGTQKPPK PVPGPEPFRK KEVVEILSHK KAYNTSILLA
910 920 930 940 950
HLKLTPGELR QVLMSMEPRR LEPAHLAQLL LFAPDADEEQ RYQAFREAPG
960 970 980 990 1000
RLSEPDQFVL QMLSVPEYKT RLRSLHFQAT LQEKTEEIRG SLECLRQASL
1010 1020 1030 1040 1050
ELKNSRKLAK ILEFVLAMGN YLNDGQPKTN KTTGFKINFL TELNSTKTVD
1060 1070 1080 1090 1100
GKSTFLHILA KSLSQHFPEL LGFAQDLPTV PLAAKVNQRA LTGDLADLHD
1110 1120 1130 1140 1150
TVSEIQVACQ SMAPSSEDRF AVVMASFLET AQPALRALDG LQREAMEELG
1160 1170 1180 1190 1200
KALAFFGEDS KATTSEAFFG IFSEFMSKFE RALSDLQAGD GPRSSGMVSP

LAW
Note: Not palmitoylated.
Length:1,203
Mass (Da):132,021
Last modified:September 5, 2006 - v1
Checksum:i79AFAA7E869731C1
GO
Isoform 2 (identifier: Q0QWG9-2) [UniParc]FASTAAdd to basket

Also known as: S-delphilin, delphilin alpha

The sequence of this isoform differs from the canonical sequence as follows:
     1-179: Missing.
     180-184: LDNLR → MSCLG

Note: When Cys-3 is mutated to Ala-3, isoform 2 is not palmitoylated anymore.
Show »
Length:1,024
Mass (Da):112,579
Checksum:iE318AFDEE02F846A
GO
Isoform 3 (identifier: Q0QWG9-3) [UniParc]FASTAAdd to basket

Also known as: delphilin beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.
     173-183: SEAQGPVLDNL → MGKDQGFSRHF

Show »
Length:1,031
Mass (Da):113,535
Checksum:i149B849E4B95AE67
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 179179Missing in isoform 2. 2 PublicationsVSP_033275Add
BLAST
Alternative sequencei1 – 172172Missing in isoform 3. 2 PublicationsVSP_033276Add
BLAST
Alternative sequencei173 – 18311SEAQGPVLDNL → MGKDQGFSRHF in isoform 3. 2 PublicationsVSP_033277Add
BLAST
Alternative sequencei180 – 1845LDNLR → MSCLG in isoform 2. 2 PublicationsVSP_033278

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ193535 mRNA. Translation: ABB04525.1.
AF099933 mRNA. Translation: AAG31020.1.
BC075624 mRNA. Translation: AAH75624.1.
AY377834 mRNA. No translation available.
AY377835 mRNA. No translation available.
CCDSiCCDS19841.1. [Q0QWG9-2]
CCDS51689.1. [Q0QWG9-1]
RefSeqiNP_001152793.1. NM_001159321.1. [Q0QWG9-1]
NP_579933.1. NM_133355.1. [Q0QWG9-2]
XP_006504713.1. XM_006504650.2.
UniGeneiMm.197460.

Genome annotation databases

EnsembliENSMUST00000010969; ENSMUSP00000010969; ENSMUSG00000010825. [Q0QWG9-2]
ENSMUST00000110733; ENSMUSP00000106361; ENSMUSG00000010825. [Q0QWG9-1]
ENSMUST00000120825; ENSMUSP00000113443; ENSMUSG00000010825. [Q0QWG9-3]
GeneIDi170935.
KEGGimmu:170935.
UCSCiuc009akd.1. mouse. [Q0QWG9-1]
uc009ake.1. mouse. [Q0QWG9-2]
uc009akf.1. mouse. [Q0QWG9-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ193535 mRNA. Translation: ABB04525.1.
AF099933 mRNA. Translation: AAG31020.1.
BC075624 mRNA. Translation: AAH75624.1.
AY377834 mRNA. No translation available.
AY377835 mRNA. No translation available.
CCDSiCCDS19841.1. [Q0QWG9-2]
CCDS51689.1. [Q0QWG9-1]
RefSeqiNP_001152793.1. NM_001159321.1. [Q0QWG9-1]
NP_579933.1. NM_133355.1. [Q0QWG9-2]
XP_006504713.1. XM_006504650.2.
UniGeneiMm.197460.

3D structure databases

ProteinModelPortaliQ0QWG9.
SMRiQ0QWG9. Positions 26-57, 267-341, 821-1182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228467. 1 interaction.
STRINGi10090.ENSMUSP00000106361.

PTM databases

iPTMnetiQ0QWG9.
PhosphoSiteiQ0QWG9.
SwissPalmiQ0QWG9.

Proteomic databases

MaxQBiQ0QWG9.
PaxDbiQ0QWG9.
PRIDEiQ0QWG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010969; ENSMUSP00000010969; ENSMUSG00000010825. [Q0QWG9-2]
ENSMUST00000110733; ENSMUSP00000106361; ENSMUSG00000010825. [Q0QWG9-1]
ENSMUST00000120825; ENSMUSP00000113443; ENSMUSG00000010825. [Q0QWG9-3]
GeneIDi170935.
KEGGimmu:170935.
UCSCiuc009akd.1. mouse. [Q0QWG9-1]
uc009ake.1. mouse. [Q0QWG9-2]
uc009akf.1. mouse. [Q0QWG9-3]

Organism-specific databases

CTDi392862.
MGIiMGI:2176213. Grid2ip.

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
KOG3528. Eukaryota.
ENOG410XSM6. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000112812.
HOVERGENiHBG107964.
InParanoidiQ0QWG9.
OMAiCQSMSPS.
OrthoDBiEOG7DJSKF.
PhylomeDBiQ0QWG9.
TreeFamiTF329416.

Miscellaneous databases

PROiQ0QWG9.
SOURCEiSearch...

Gene expression databases

BgeeiQ0QWG9.
GenevisibleiQ0QWG9. MM.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015425. FH2_Formin.
IPR001478. PDZ.
[Graphical view]
PfamiPF02181. FH2. 1 hit.
PF00595. PDZ. 2 hits.
[Graphical view]
SMARTiSM00498. FH2. 1 hit.
SM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS51444. FH2. 1 hit.
PS50106. PDZ. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Delphilin: a novel PDZ and formin homology domain-containing protein that synaptically colocalizes and interacts with glutamate receptor delta 2 subunit."
    Miyagi Y., Yamashita T., Fukaya M., Sonoda T., Okuno T., Yamada K., Watanabe M., Nagashima Y., Aoki I., Okuda K., Mishina M., Kawamoto S.
    J. Neurosci. 22:803-814(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, INTERACTION WITH GRID2.
    Strain: BALB/cJ.
  2. "Characterization of the delta2 glutamate receptor-binding protein delphilin: splicing variants with differential palmitoylation and an additional PDZ domain."
    Matsuda K., Matsuda S., Gladding C.M., Yuzaki M.
    J. Biol. Chem. 281:25577-25587(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, PALMITOYLATION AT CYS-3 (ISOFORM 2), INTERACTION WITH GRID2.
    Tissue: Cerebellum.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Identification and characterization of a novel Delphilin variant with an alternative N-terminus."
    Yamashita T., Miyagi Y., Ono M., Ito H., Watanabe K., Sonoda T., Tsuzuki K., Ozawa S., Aoki I., Okuda K., Mishina M., Kawamoto S.
    Brain Res. Mol. Brain Res. 141:83-94(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-184 (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 1-183 (ISOFORM 3), INTERACTION WITH GRID2, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
  5. "Binding of glutamate receptor delta2 to its scaffold protein, Delphilin, is regulated by PKA."
    Sonoda T., Mochizuki C., Yamashita T., Watanabe-Kaneko K., Miyagi Y., Shigeri Y., Yazama F., Okuda K., Kawamoto S.
    Biochem. Biophys. Res. Commun. 350:748-752(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRID2.
  6. "The extreme C-terminus of GluRdelta2 is essential for induction of long-term depression in cerebellar slices."
    Kohda K., Kakegawa W., Matsuda S., Nakagami R., Kakiya N., Yuzaki M.
    Eur. J. Neurosci. 25:1357-1362(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRID2.
  7. "The synaptic scaffolding protein Delphilin interacts with monocarboxylate transporter 2."
    Watanabe-Kaneko K., Sonoda T., Miyagi Y., Yamashita T., Okuda K., Kawamoto S.
    NeuroReport 18:489-493(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLC16A7.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303; SER-572; SER-613; SER-644 AND SER-647, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiGRD2I_MOUSE
AccessioniPrimary (citable) accession number: Q0QWG9
Secondary accession number(s): Q6DID0, Q9ESJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 5, 2006
Last modified: June 8, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Isoform 1 shows a punctate distribution throughout the cytoplasm when expressed in COS cells, whereas isoform 2 is enriched at the edges of the plasma membranes. When expressed in cultured hippocampal neurons, isoform 1 forms clusters mainly in the dendritic shafts, whereas isoform 2 is preferentially expressed in spines.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.