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Q0QLF2

- NDLMS_EUBBA

UniProt

Q0QLF2 - NDLMS_EUBBA

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Protein

Nicotinate dehydrogenase large molybdopterin subunit

Gene

ndhL

Organism
Eubacterium barkeri (Clostridium barkeri)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of nicotinate to 6-hydroxynicotinate. Also active against 2-pyrazinecarboxylic acid, but inactive against other nicotinate analogs.1 Publication

Catalytic activityi

Nicotinate + H2O + NADP+ = 6-hydroxynicotinate + NADPH.1 Publication

Cofactori

Binds 1 hydroxyoxoselanylmolybdenum molybdopterin cytosine dinucleotide per heterotetramer. The cofactor is bound between the ndhL and ndhM subunits.1 Publication

Enzyme regulationi

Reversibly inactivated by selenide and sulfide. Not inhibited by cyanide.1 Publication

pH dependencei

Most stable at pH 8.0. Unstable at acidic pH values.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei208 – 2081Hydroxyoxoselanylmolybdenum molybdopterin cytosine dinucleotide1 Publication

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nicotinate dehydrogenase activity Source: UniProtKB

GO - Biological processi

  1. cofactor catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, Molybdenum, NADP, Selenium

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11705.
UniPathwayiUPA01010; UER01011.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate dehydrogenase large molybdopterin subunitImported (EC:1.17.1.5)
Short name:
NDH1 Publication
Alternative name(s):
Nicotinic acid hydroxylase large molybdopterin subunit1 Publication
Short name:
NAH1 Publication
Gene namesi
Name:ndhL
OrganismiEubacterium barkeri (Clostridium barkeri)
Taxonomic identifieri1528 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesEubacteriaceaeEubacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 425424Nicotinate dehydrogenase large molybdopterin subunit1 PublicationPRO_0000404243Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of NDHM, NDHL, NDHS and NDHF. Dimer of heterotetramers.2 Publications

Protein-protein interaction databases

DIPiDIP-48913N.

Structurei

Secondary structure

1
425
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 215
Helixi28 – 303
Beta strandi37 – 437
Beta strandi45 – 5511
Helixi57 – 604
Beta strandi65 – 695
Helixi71 – 733
Beta strandi82 – 843
Beta strandi101 – 1099
Helixi110 – 11910
Beta strandi121 – 1266
Helixi133 – 1375
Beta strandi149 – 15911
Helixi161 – 1666
Beta strandi169 – 17810
Beta strandi190 – 1956
Beta strandi201 – 2055
Helixi210 – 22011
Helixi225 – 2273
Beta strandi228 – 2325
Turni239 – 2424
Helixi248 – 25811
Beta strandi262 – 2654
Helixi268 – 2747
Beta strandi281 – 2899
Beta strandi295 – 30915
Helixi313 – 32311
Beta strandi333 – 3419
Beta strandi343 – 3453
Turni351 – 3544
Helixi355 – 37218
Helixi377 – 3848
Helixi404 – 41916

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HRDX-ray2.20A/E1-425[»]
ProteinModelPortaliQ0QLF2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ0QLF2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni238 – 2403Hydroxyoxoselanylmolybdenum molybdopterin cytosine dinucleotide binding1 Publication

Sequence similaritiesi

Belongs to the xanthine dehydrogenase family.Sequence Analysis

Family and domain databases

Gene3Di3.30.365.10. 4 hits.
3.90.1170.50. 1 hit.
InterProiIPR000674. Ald_Oxase/Xan_DH_a/b.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
[Graphical view]
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF54665. SSF54665. 1 hit.
SSF56003. SSF56003. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0QLF2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKDYQVLGK NKVKVDSLEK VMGTAKFAAD YSFPDMLYAG VFRSTVPHAR
60 70 80 90 100
IVSLDLSKAR AIDGVEAVLD YHAIPGKNRF GIIIKDEPCL VDDKVRRYGD
110 120 130 140 150
AIAVVAAQTP DLVQEALDAI TIEYEELEGI FTMERALEED SPAIHGDTNI
160 170 180 190 200
HQVKHLEYGD VDAAFKQCDI VVEDTYSTHR LTHMFIEPDA GVSYYDNEGM
210 220 230 240 250
LTVVVSTQNP HYDRGEVAGM LALPNSKVRI IQATTGGGFG GKLDLSVQCH
260 270 280 290 300
CALLTYHTKK PVKMVRSREE STTVSSKRHP MTMHCKTGAT KDGRLQAVQV
310 320 330 340 350
EMFGDTGAYA SYGPAVITRA TVHCMGPYVV PNVRVDAKFV YTNNPMSGAF
360 370 380 390 400
RGFGVPQASV CHEGQMNALA KALGMDPIDI RILNAHQVGA KLATGQVLEN
410 420
SVGLIETLEK AREKAVEVMG YEKTR
Length:425
Mass (Da):46,488
Last modified:September 5, 2006 - v1
Checksum:iF8270B4ABECD71A5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ310789 Genomic DNA. Translation: ABC88398.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ310789 Genomic DNA. Translation: ABC88398.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3HRD X-ray 2.20 A/E 1-425 [» ]
ProteinModelPortali Q0QLF2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48913N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA01010 ; UER01011 .
BioCyci MetaCyc:MONOMER-11705.

Miscellaneous databases

EvolutionaryTracei Q0QLF2.

Family and domain databases

Gene3Di 3.30.365.10. 4 hits.
3.90.1170.50. 1 hit.
InterProi IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
[Graphical view ]
Pfami PF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
[Graphical view ]
SMARTi SM01008. Ald_Xan_dh_C. 1 hit.
[Graphical view ]
SUPFAMi SSF54665. SSF54665. 1 hit.
SSF56003. SSF56003. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri."
    Alhapel A., Darley D.J., Wagener N., Eckel E., Elsner N., Pierik A.J.
    Proc. Natl. Acad. Sci. U.S.A. 103:12341-12346(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
    Strain: ATCC 25849 / DSM 1223 / JCM 1389 / NCIB 10623Imported.
  2. "Properties of the selenium- and molybdenum-containing nicotinic acid hydroxylase from Clostridium barkeri."
    Gladyshev V.N., Khangulov S.V., Stadtman T.C.
    Biochemistry 35:212-223(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-26, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT.
    Strain: ATCC 25849 / DSM 1223 / JCM 1389 / NCIB 106231 Publication.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH COFACTOR; NDHM; NDHS AND NDHF, SUBUNIT.
    Strain: ATCC 25849 / DSM 1223 / JCM 1389 / NCIB 106231 Publication.

Entry informationi

Entry nameiNDLMS_EUBBA
AccessioniPrimary (citable) accession number: Q0QLF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: September 5, 2006
Last modified: October 1, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3