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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

SDHA

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei217 – 2171FADBy similarity
Binding sitei238 – 2381SubstrateBy similarity
Binding sitei250 – 2501SubstrateBy similarity
Active sitei282 – 2821Proton acceptorBy similarity
Binding sitei349 – 3491SubstrateBy similarity
Binding sitei382 – 3821FADBy similarity
Binding sitei393 – 3931SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156FADBy similarity
Nucleotide bindingi33 – 4816FADBy similarityAdd
BLAST
Nucleotide bindingi398 – 3992FADBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. electron transport chain Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialBy similarity (EC:1.3.5.1)
Alternative name(s):
Flavoprotein subunit of complex IIBy similarity
Short name:
FpBy similarity
Gene namesi
Name:SDHAImported
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›551›551Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialPRO_0000394746Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei41 – 411Tele-8alpha-FAD histidineBy similarity
Modified residuei109 – 1091N6-acetyllysineBy similarity
Modified residuei121 – 1211N6-acetyllysine; alternateBy similarity
Modified residuei121 – 1211N6-succinyllysine; alternateBy similarity
Modified residuei124 – 1241N6-acetyllysineBy similarity
Modified residuei157 – 1571Phosphotyrosine; by SRCBy similarity
Modified residuei192 – 1921N6-acetyllysine; alternateBy similarity
Modified residuei192 – 1921N6-succinyllysine; alternateBy similarity
Modified residuei277 – 2771N6-acetyllysine; alternateBy similarity
Modified residuei277 – 2771N6-succinyllysine; alternateBy similarity
Modified residuei365 – 3651N6-acetyllysineBy similarity
Modified residuei422 – 4221N6-acetyllysineBy similarity
Modified residuei427 – 4271N6-acetyllysine; alternateBy similarity
Modified residuei427 – 4271N6-succinyllysine; alternateBy similarity
Modified residuei440 – 4401N6-acetyllysine; alternateBy similarity
Modified residuei440 – 4401N6-succinyllysine; alternateBy similarity
Modified residuei459 – 4591N6-acetyllysineBy similarity
Modified residuei480 – 4801N6-acetyllysine; alternateBy similarity
Modified residuei480 – 4801N6-succinyllysine; alternateBy similarity
Modified residuei489 – 4891N6-acetyllysine; alternateBy similarity
Modified residuei489 – 4891N6-succinyllysine; alternateBy similarity
Modified residuei492 – 4921N6-acetyllysineBy similarity
Modified residuei540 – 5401N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylation at Tyr-157 is important for efficient electron transfer in complex II and the prevention of ROS generation.By similarity
Deacetylated by SIRT3.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ0QF17.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interacts with SDHAF2/SDH5; interaction is required for FAD attachment (By similarity). Interacts with TRAP1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ0QF17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG001461.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q0QF17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DHEFDAVVVG AGGAGLRAAF GLSEAGFNTA CGTKLFPTRS HTVAAQGGIN
60 70 80 90 100
AALGNMEEDN WRWHFYDTVK GSDWLGDQDA IHYMTEQAPA SVVELENYGM
110 120 130 140 150
PFSTTEDGKI YQRAFGGQSL KFGKGGQAHR SCCVADRTGH SLLHTLYGRS
160 170 180 190 200
LRYDTSYFVE NFALDLLMEN GECRGVIALC IEDGSIHRIR AKNTVIATGG
210 220 230 240 250
YGRTYFSCTS AHTSTGDGTA MVTRAGLPCQ DLEFIQFHPT GIYGAGCLIT
260 270 280 290 300
EGCRGEGGIL INSQGERFME RYAPVAKDLA SRDVVSRSMT LEIREGRSWG
310 320 330 340 350
PEKDHVYLQL HHLPPEQLAT RLPGISETAM IFAGVDVTKE PIPVLPTVHY
360 370 380 390 400
NMGGIPTNYK GQVLKHVNGQ DQVVPGLYAC GEAACASVHG AIRLGANSLL
410 420 430 440 450
DLVVFGRACA LSIAESCSPG DKVPPIKANA GEESVMNLDK LRFADGSIRT
460 470 480 490 500
SELRLSMQKS MQSHAAVFRV GSVLQEGCEK ISQLYGELKH LKTFDRGMVW
510 520 530 540 550
NTDLVETLEL QNLMLCALQT IYGAEARKES RGAHAREDYK VRVDEYDYYX

A
Length:551
Mass (Da):60,045
Last modified:September 5, 2006 - v1
Checksum:i13CB219D31B9B678
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11Imported
Non-terminal residuei551 – 5511Imported

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ402977 mRNA. Translation: ABD77310.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ402977 mRNA. Translation: ABD77310.1.

3D structure databases

ProteinModelPortaliQ0QF17.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ0QF17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG001461.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Housekeeping genes for phylogenetic analysis of eutherian relationships."
    Kullberg M., Nilsson M.A., Arnason U., Harley E.H., Janke A.
    Mol. Biol. Evol. 23:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: LiverImported.
  2. "Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa."
    Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S.
    Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSDHA_MESAU
AccessioniPrimary (citable) accession number: Q0QF17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: September 5, 2006
Last modified: March 4, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.