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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

SDHA

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.By similarity

Cofactori

FADBy similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei217FADBy similarity1
Binding sitei238SubstrateBy similarity1
Binding sitei250SubstrateBy similarity1
Active sitei282Proton acceptorBy similarity1
Binding sitei349SubstrateBy similarity1
Binding sitei382FADBy similarity1
Binding sitei393SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15FADBy similarity6
Nucleotide bindingi33 – 48FADBy similarityAdd BLAST16
Nucleotide bindingi398 – 399FADBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialBy similarity (EC:1.3.5.1By similarity)
Alternative name(s):
Flavoprotein subunit of complex IIBy similarity
Short name:
FpBy similarity
Gene namesi
Name:SDHAImported
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

  • Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000394746‹1 – ›551Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialAdd BLAST›551

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41Tele-8alpha-FAD histidineBy similarity1
Modified residuei109N6-acetyllysineBy similarity1
Modified residuei121N6-acetyllysine; alternateBy similarity1
Modified residuei121N6-succinyllysine; alternateBy similarity1
Modified residuei124N6-acetyllysineBy similarity1
Modified residuei157Phosphotyrosine; by SRCBy similarity1
Modified residuei192N6-acetyllysine; alternateBy similarity1
Modified residuei192N6-succinyllysine; alternateBy similarity1
Modified residuei277N6-acetyllysine; alternateBy similarity1
Modified residuei277N6-succinyllysine; alternateBy similarity1
Modified residuei365N6-acetyllysineBy similarity1
Modified residuei422N6-acetyllysineBy similarity1
Modified residuei427N6-acetyllysine; alternateBy similarity1
Modified residuei427N6-succinyllysine; alternateBy similarity1
Modified residuei440N6-acetyllysine; alternateBy similarity1
Modified residuei440N6-succinyllysine; alternateBy similarity1
Modified residuei459N6-acetyllysineBy similarity1
Modified residuei480N6-acetyllysine; alternateBy similarity1
Modified residuei480N6-succinyllysine; alternateBy similarity1
Modified residuei489N6-acetyllysine; alternateBy similarity1
Modified residuei489N6-succinyllysine; alternateBy similarity1
Modified residuei492N6-acetyllysineBy similarity1
Modified residuei540N6-acetyllysineBy similarity1

Post-translational modificationi

Acetylated. Deacetylated by SIRT3.By similarity
Phosphorylation at Tyr-157 is important for efficient electron transfer in complex II and the prevention of ROS generation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ0QF17.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD (By similarity). Interacts with SDHAF2/SDH5; interaction is required for FAD attachment (By similarity). Interacts with TRAP1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ0QF17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG001461.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR030664. SdhA/FrdA/AprA.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000171. SDHA_APRA_LASPO. 1 hit.
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q0QF17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DHEFDAVVVG AGGAGLRAAF GLSEAGFNTA CGTKLFPTRS HTVAAQGGIN
60 70 80 90 100
AALGNMEEDN WRWHFYDTVK GSDWLGDQDA IHYMTEQAPA SVVELENYGM
110 120 130 140 150
PFSTTEDGKI YQRAFGGQSL KFGKGGQAHR SCCVADRTGH SLLHTLYGRS
160 170 180 190 200
LRYDTSYFVE NFALDLLMEN GECRGVIALC IEDGSIHRIR AKNTVIATGG
210 220 230 240 250
YGRTYFSCTS AHTSTGDGTA MVTRAGLPCQ DLEFIQFHPT GIYGAGCLIT
260 270 280 290 300
EGCRGEGGIL INSQGERFME RYAPVAKDLA SRDVVSRSMT LEIREGRSWG
310 320 330 340 350
PEKDHVYLQL HHLPPEQLAT RLPGISETAM IFAGVDVTKE PIPVLPTVHY
360 370 380 390 400
NMGGIPTNYK GQVLKHVNGQ DQVVPGLYAC GEAACASVHG AIRLGANSLL
410 420 430 440 450
DLVVFGRACA LSIAESCSPG DKVPPIKANA GEESVMNLDK LRFADGSIRT
460 470 480 490 500
SELRLSMQKS MQSHAAVFRV GSVLQEGCEK ISQLYGELKH LKTFDRGMVW
510 520 530 540 550
NTDLVETLEL QNLMLCALQT IYGAEARKES RGAHAREDYK VRVDEYDYYX

A
Length:551
Mass (Da):60,045
Last modified:September 5, 2006 - v1
Checksum:i13CB219D31B9B678
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1Imported1
Non-terminal residuei551Imported1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ402977 mRNA. Translation: ABD77310.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ402977 mRNA. Translation: ABD77310.1.

3D structure databases

ProteinModelPortaliQ0QF17.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ0QF17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG001461.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR030664. SdhA/FrdA/AprA.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000171. SDHA_APRA_LASPO. 1 hit.
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDHA_MESAU
AccessioniPrimary (citable) accession number: Q0QF17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: September 5, 2006
Last modified: March 16, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.