ID GATA4_CANLF Reviewed; 442 AA. AC Q0Q0E4; DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot. DT 05-SEP-2006, sequence version 1. DT 27-MAR-2024, entry version 122. DE RecName: Full=Transcription factor GATA-4; DE AltName: Full=GATA-binding factor 4; GN Name=GATA4; OS Canis lupus familiaris (Dog) (Canis familiaris). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis. OX NCBI_TaxID=9615; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RA Hyun C., Lee S.-A., Lee S.-G.; RT "Molecular screening of GATA4 gene in canine atrial septal defects."; RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Transcriptional activator that binds to the consensus CC sequence 5'-AGATAG-3' and plays a key role in cardiac development. In CC cooperation with TBX5, it binds to cardiac super-enhancers and promotes CC cardiomyocyte gene expression, while it down-regulates endocardial and CC endothelial gene expression. Involved in bone morphogenetic protein CC (BMP)-mediated induction of cardiac-specific gene expression. Binds to CC BMP response element (BMPRE) DNA sequences within cardiac activating CC regions. Acts as a transcriptional activator of ANF in cooperation with CC NKX2-5. Promotes cardiac myocyte enlargement. Required during CC testicular development. May play a role in sphingolipid signaling by CC regulating the expression of sphingosine-1-phosphate degrading enzyme, CC sphingosine-1-phosphate lyase. {ECO:0000250|UniProtKB:P43694, CC ECO:0000250|UniProtKB:P46152, ECO:0000250|UniProtKB:Q08369}. CC -!- SUBUNIT: Interacts with ZNF260 (By similarity). Interacts with the CC homeobox domain of NKX2-5 through its C-terminal zinc finger. Also CC interacts with JARID2 which represses its ability to activate CC transcription of ANF. Interacts (via the second Zn finger) with NFATC4 CC (By similarity). Interacts with LMCD1 (By similarity). Forms a complex CC made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates CC hypertrophy in cardiomyocytes. Interacts with NR5A1, ZFPM2 and TBX5. CC Interacts with TBX18. {ECO:0000250|UniProtKB:P43694, CC ECO:0000250|UniProtKB:Q08369}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P43694}. CC -!- PTM: Methylation at Lys-300 attenuates transcriptional activity. CC {ECO:0000250}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DQ666280; ABG75570.1; -; mRNA. DR RefSeq; NP_001041577.1; NM_001048112.1. DR RefSeq; XP_005635679.1; XM_005635622.2. DR RefSeq; XP_013962935.1; XM_014107460.1. DR RefSeq; XP_013962936.1; XM_014107461.1. DR RefSeq; XP_013962937.1; XM_014107462.1. DR RefSeq; XP_013962938.1; XM_014107463.1. DR AlphaFoldDB; Q0Q0E4; -. DR SMR; Q0Q0E4; -. DR STRING; 9615.ENSCAFP00000038219; -. DR PaxDb; 9612-ENSCAFP00000038219; -. DR Ensembl; ENSCAFT00000048684.3; ENSCAFP00000038219.1; ENSCAFG00000029087.3. DR Ensembl; ENSCAFT00030032771.1; ENSCAFP00030028589.1; ENSCAFG00030017775.1. DR Ensembl; ENSCAFT00805027409; ENSCAFP00805021524; ENSCAFG00805015092. DR Ensembl; ENSCAFT00845034032.1; ENSCAFP00845026636.1; ENSCAFG00845019258.1. DR GeneID; 486079; -. DR KEGG; cfa:486079; -. DR CTD; 2626; -. DR VEuPathDB; HostDB:ENSCAFG00845019258; -. DR VGNC; VGNC:54949; GATA4. DR eggNOG; KOG1601; Eukaryota. DR GeneTree; ENSGT00940000158349; -. DR HOGENOM; CLU_027524_0_0_1; -. DR InParanoid; Q0Q0E4; -. DR OMA; GIMTSNH; -. DR OrthoDB; 685518at2759; -. DR TreeFam; TF315391; -. DR Reactome; R-CFA-983231; Factors involved in megakaryocyte development and platelet production. DR Proteomes; UP000002254; Chromosome 25. DR Proteomes; UP000694429; Chromosome 25. DR Proteomes; UP000694542; Unplaced. DR Proteomes; UP000805418; Chromosome 25. DR Bgee; ENSCAFG00000029087; Expressed in jejunum and 18 other cell types or tissues. DR GO; GO:0016604; C:nuclear body; IEA:Ensembl. DR GO; GO:0005634; C:nucleus; ISS:UniProtKB. DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IEA:Ensembl. DR GO; GO:0070410; F:co-SMAD binding; IEA:Ensembl. DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB. DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl. DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central. DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:Ensembl. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0003180; P:aortic valve morphogenesis; IEA:Ensembl. DR GO; GO:0003290; P:atrial septum secundum morphogenesis; IEA:Ensembl. DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central. DR GO; GO:0003197; P:endocardial cushion development; IEA:Ensembl. DR GO; GO:0060575; P:intestinal epithelial cell differentiation; IEA:Ensembl. DR GO; GO:0008584; P:male gonad development; IEA:Ensembl. DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central. DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; ISS:UniProtKB. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central. DR GO; GO:0009410; P:response to xenobiotic stimulus; IEA:Ensembl. DR CDD; cd00202; ZnF_GATA; 2. DR Gene3D; 3.30.50.10; Erythroid Transcription Factor GATA-1, subunit A; 2. DR InterPro; IPR008013; GATA_N. DR InterPro; IPR016375; TF_GATA_4/5/6. DR InterPro; IPR039355; Transcription_factor_GATA. DR InterPro; IPR000679; Znf_GATA. DR InterPro; IPR013088; Znf_NHR/GATA. DR PANTHER; PTHR10071; TRANSCRIPTION FACTOR GATA FAMILY MEMBER; 1. DR PANTHER; PTHR10071:SF154; TRANSCRIPTION FACTOR GATA-4; 1. DR Pfam; PF00320; GATA; 2. DR Pfam; PF05349; GATA-N; 1. DR PIRSF; PIRSF003028; TF_GATA_4/5/6; 1. DR PRINTS; PR00619; GATAZNFINGER. DR SMART; SM00401; ZnF_GATA; 2. DR SUPFAM; SSF57716; Glucocorticoid receptor-like (DNA-binding domain); 2. DR PROSITE; PS00344; GATA_ZN_FINGER_1; 2. DR PROSITE; PS50114; GATA_ZN_FINGER_2; 2. PE 2: Evidence at transcript level; KW Activator; DNA-binding; Metal-binding; Methylation; Nucleus; KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; KW Zinc-finger. FT CHAIN 1..442 FT /note="Transcription factor GATA-4" FT /id="PRO_0000289588" FT ZN_FING 217..241 FT /note="GATA-type 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094" FT ZN_FING 271..295 FT /note="GATA-type 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094" FT REGION 62..107 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 315..379 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 408..442 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 340..362 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 408..429 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 300 FT /note="N6-methyllysine; by EZH2" FT /evidence="ECO:0000250|UniProtKB:Q08369" SQ SEQUENCE 442 AA; 44487 MW; 1CF7F807586FC991 CRC64; MYQSLAMAAN HGPPPGAYEA GGPGAFMHGA GAASSPVYVP TPRVPSSVLG LSYLQGGGGA AASGASSGGG SGGAPSAAGP GAQQGSPGWS QAGADGAAYT PPPVSPRFSF PGTTGSLAAA AAAAAAREAA AYGGGGGAAG AGLAGREQYG RAGFAGSYSS PYPAYMADVG ASWAAAAAAS AGPFDSPVLH SLPGRANPAA RHPNLDMFDD FSEGRECVNC GAMSTPLWRR DGTGHYLCNA CGLYHKMNGI NRPLIKPQRR LSASRRVGLS CANCQTTTTT LWRRNAEGEP VCNACGLYMK LHGVPRPLAM RKEGIQTRKR KPKNLNKSKT PAGPSGGESL PPASSASSNS SNVATSSSEE MRPIKTEPGL SSHYGHSSSM SQTFSVSAMS GHGPSIHPVL SALKLSPQGY TSSVSQSPQA SSKQDPWNSL ALADSHGDII TA //