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Protein

NAD(P)H-hydrate epimerase

Gene

NAXE

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.UniRule annotation

Catalytic activityi

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi120PotassiumUniRule annotation1
Metal bindingi185PotassiumUniRule annotation1
Binding sitei218NAD(P)HXUniRule annotation1
Metal bindingi221PotassiumUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-hydrate epimeraseUniRule annotation (EC:5.1.99.6)
Alternative name(s):
Apolipoprotein A-I-binding proteinUniRule annotation
Short name:
AI-BPUniRule annotation
NAD(P)HX epimeraseBy similarity
Gene namesi
Name:NAXEBy similarity
Synonyms:AIBPUniRule annotation, APOA1BPUniRule annotation
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

  • Mitochondrion UniRule annotation
  • Secreted UniRule annotation

  • Note: In sperm, secretion gradually increases during capacitation.UniRule annotation

GO - Cellular componenti

  • extracellular space Source: BHF-UCL
  • mitochondrion Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 59MitochondrionUniRule annotationAdd BLAST59
ChainiPRO_000029242460 – 288NAD(P)H-hydrate epimeraseAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei144N6-succinyllysineBy similarity1

Post-translational modificationi

Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation.UniRule annotation

Proteomic databases

PaxDbiQ0PIT9.
PeptideAtlasiQ0PIT9.
PRIDEiQ0PIT9.

Interactioni

Subunit structurei

Homodimer. Interacts with APOA1 and APOA2.UniRule annotation

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000006906.

Structurei

3D structure databases

ProteinModelPortaliQ0PIT9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 275YjeF N-terminalUniRule annotationAdd BLAST211

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 123NAD(P)HXUniRule annotation5
Regioni189 – 195NAD(P)HXUniRule annotation7

Sequence similaritiesi

Belongs to the NnrE/AIBP family.UniRule annotation
Contains 1 YjeF N-terminal domain.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiQ0PIT9.
KOiK17759.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase. 1 hit.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0PIT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLRALLGL GLPVAGSRLP RVRVQAGACR ARPTWWGPQR LISGGRGDVE
60 70 80 90 100
GMASSAVKYL SQEEAQAVDQ ELFNEYQFSV DQLMELAGLS CATAIAKVYP
110 120 130 140 150
PTSLSRSPPT VLVICGPGNN GGDGLVCARH LKLFGYHPTI YYPKRPNKPL
160 170 180 190 200
FTALVTQCQK MDIPFLDEMP SEPTLIDELY ELVVDAIFGF SFKGEVREPF
210 220 230 240 250
RSILSVLNGL TVPIASIDIP SGWDVERGNS GGIQPDLLIS LTAPKKSAAQ
260 270 280
FTGRYHYLGG RFVPPALEKK YQLNLPPYPD TECVYRLQ
Length:288
Mass (Da):31,501
Last modified:October 31, 2006 - v1
Checksum:iC0E945E3EDF22385
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ826508 mRNA. Translation: ABH01264.1.
RefSeqiNP_001072132.1. NM_001078664.1.
UniGeneiSsc.19257.

Genome annotation databases

GeneIDi780405.
KEGGissc:780405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ826508 mRNA. Translation: ABH01264.1.
RefSeqiNP_001072132.1. NM_001078664.1.
UniGeneiSsc.19257.

3D structure databases

ProteinModelPortaliQ0PIT9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000006906.

Proteomic databases

PaxDbiQ0PIT9.
PeptideAtlasiQ0PIT9.
PRIDEiQ0PIT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi780405.
KEGGissc:780405.

Organism-specific databases

CTDi128240.

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiQ0PIT9.
KOiK17759.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase. 1 hit.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNRE_PIG
AccessioniPrimary (citable) accession number: Q0PIT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: October 31, 2006
Last modified: November 2, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.