Q0PBE9 (LSPA_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	Cj0361

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000097243
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	39 – 58	20	Helical; Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Transmembrane	89 – 109	21	Helical; Potential
	Transmembrane	122 – 142	21	Helical; Potential
Sites
	Active site	104	1	By similarity
	Active site	130	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0PBE9 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: DF2E6E4107F41733	FASTA	156	18,327
	10 20 30 40 50 60 MAKTFKFIFY FWGAFVLVFA LDQWVKSLTL AGFRWQSEYL DLTYALNTGV AFSMLSFLEH 70 80 90 100 110 120 NLKYLHLALI GVLFIYLFWQ RTLLKTHSIA FGMMLGAGVS NLLDRFIHGG VVDMFFWHKW 130 140 150 FNFAIFNVAD VMINISVALI LIQEIFKKRK KDDRMD

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL34511.1.
PIR	G81378.
RefSeq	YP_002343798.1. NC_002163.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q0PBE9. 7 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	904684.
GenomeReviews	Gene locus Cj0361 in contig AL111168_GR.
KEGG	cje:Cj0361.
PATRIC	20057637. VBICamJej33762_0352.
Phylogenomic databases
HOGENOM	HBG724422.
OMA	GVAFSMF.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0361-MONOMER.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_CAMJE

Accession

Primary (citable) accession number: Q0PBE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 19, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program