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Protein

Bifunctional protein FolD

Gene

folD

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei189 – 1891NADP; via amide nitrogenUniRule annotation1 Publication
Binding sitei230 – 2301NADP; via amide nitrogenUniRule annotation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 1663NADPUniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-829-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:Cj0855
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Bifunctional protein FolDPRO_0000268304Add
BLAST

Proteomic databases

PaxDbiQ0PA35.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ0PA35. 36 interactions.
MINTiMINT-6484879.
STRINGi192222.Cj0855.

Structurei

Secondary structure

1
282
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 2520Combined sources
Turni26 – 283Combined sources
Beta strandi32 – 398Combined sources
Helixi42 – 5817Combined sources
Beta strandi61 – 677Combined sources
Helixi73 – 8513Combined sources
Beta strandi91 – 944Combined sources
Helixi104 – 1107Combined sources
Helixi113 – 1153Combined sources
Helixi122 – 1298Combined sources
Helixi139 – 15012Combined sources
Beta strandi159 – 1635Combined sources
Turni167 – 1693Combined sources
Helixi170 – 17910Combined sources
Beta strandi183 – 1875Combined sources
Helixi194 – 1985Combined sources
Beta strandi202 – 2065Combined sources
Helixi216 – 2183Combined sources
Beta strandi223 – 2275Combined sources
Helixi244 – 2474Combined sources
Turni248 – 2503Combined sources
Beta strandi251 – 2544Combined sources
Beta strandi257 – 2604Combined sources
Helixi261 – 27818Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3P2OX-ray2.23A/B1-282[»]
ProteinModelPortaliQ0PA35.
SMRiQ0PA35. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ0PA35.

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0PA35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLDGKALS AKIKEELKEK NQFLKSKGIE SCLAVILVGD NPASQTYVKS
60 70 80 90 100
KAKACEECGI KSLVYHLNEN ITQNELLALI NTLNHDDSVH GILVQLPLPD
110 120 130 140 150
HICKDLILES IISSKDVDGF HPINVGYLNL GLESGFLPCT PLGVMKLLKA
160 170 180 190 200
YEIDLEGKDA VIIGASNIVG RPMATMLLNA GATVSVCHIK TKDLSLYTRQ
210 220 230 240 250
ADLIIVAAGC VNLLRSDMVK EGVIVVDVGI NRLESGKIVG DVDFEEVSKK
260 270 280
SSYITPVPGG VGPMTIAMLL ENTVKSAKNR LN
Length:282
Mass (Da):30,349
Last modified:September 19, 2006 - v1
Checksum:iA692A4469F5B9AEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34983.1.
PIRiG81358.
RefSeqiWP_002864845.1. NC_002163.1.
YP_002344262.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34983; CAL34983; Cj0855.
GeneIDi905155.
KEGGicje:Cj0855.
PATRICi20058658. VBICamJej33762_0843.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34983.1.
PIRiG81358.
RefSeqiWP_002864845.1. NC_002163.1.
YP_002344262.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3P2OX-ray2.23A/B1-282[»]
ProteinModelPortaliQ0PA35.
SMRiQ0PA35. Positions 2-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ0PA35. 36 interactions.
MINTiMINT-6484879.
STRINGi192222.Cj0855.

Proteomic databases

PaxDbiQ0PA35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34983; CAL34983; Cj0855.
GeneIDi905155.
KEGGicje:Cj0855.
PATRICi20058658. VBICamJej33762_0843.

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciCJEJ192222:GJTS-829-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ0PA35.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOLD_CAMJE
AccessioniPrimary (citable) accession number: Q0PA35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 19, 2006
Last modified: September 7, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.