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Protein

Bifunctional protein FolD

Gene

folD

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189NADP; via amide nitrogenUniRule annotation1 Publication1
Binding sitei230NADP; via amide nitrogenUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi164 – 166NADPUniRule annotation1 Publication3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-796-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:Cj0855
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002683041 – 282Bifunctional protein FolDAdd BLAST282

Proteomic databases

PaxDbiQ0PA35.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ0PA35. 36 interactors.
MINTiMINT-6484879.
STRINGi192222.Cj0855.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 25Combined sources20
Turni26 – 28Combined sources3
Beta strandi32 – 39Combined sources8
Helixi42 – 58Combined sources17
Beta strandi61 – 67Combined sources7
Helixi73 – 85Combined sources13
Beta strandi91 – 94Combined sources4
Helixi104 – 110Combined sources7
Helixi113 – 115Combined sources3
Helixi122 – 129Combined sources8
Helixi139 – 150Combined sources12
Beta strandi159 – 163Combined sources5
Turni167 – 169Combined sources3
Helixi170 – 179Combined sources10
Beta strandi183 – 187Combined sources5
Helixi194 – 198Combined sources5
Beta strandi202 – 206Combined sources5
Helixi216 – 218Combined sources3
Beta strandi223 – 227Combined sources5
Helixi244 – 247Combined sources4
Turni248 – 250Combined sources3
Beta strandi251 – 254Combined sources4
Beta strandi257 – 260Combined sources4
Helixi261 – 278Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P2OX-ray2.23A/B1-282[»]
ProteinModelPortaliQ0PA35.
SMRiQ0PA35.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ0PA35.

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0PA35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLDGKALS AKIKEELKEK NQFLKSKGIE SCLAVILVGD NPASQTYVKS
60 70 80 90 100
KAKACEECGI KSLVYHLNEN ITQNELLALI NTLNHDDSVH GILVQLPLPD
110 120 130 140 150
HICKDLILES IISSKDVDGF HPINVGYLNL GLESGFLPCT PLGVMKLLKA
160 170 180 190 200
YEIDLEGKDA VIIGASNIVG RPMATMLLNA GATVSVCHIK TKDLSLYTRQ
210 220 230 240 250
ADLIIVAAGC VNLLRSDMVK EGVIVVDVGI NRLESGKIVG DVDFEEVSKK
260 270 280
SSYITPVPGG VGPMTIAMLL ENTVKSAKNR LN
Length:282
Mass (Da):30,349
Last modified:September 19, 2006 - v1
Checksum:iA692A4469F5B9AEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34983.1.
PIRiG81358.
RefSeqiWP_002864845.1. NC_002163.1.
YP_002344262.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34983; CAL34983; Cj0855.
GeneIDi905155.
KEGGicje:Cj0855.
PATRICi20058658. VBICamJej33762_0843.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34983.1.
PIRiG81358.
RefSeqiWP_002864845.1. NC_002163.1.
YP_002344262.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P2OX-ray2.23A/B1-282[»]
ProteinModelPortaliQ0PA35.
SMRiQ0PA35.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ0PA35. 36 interactors.
MINTiMINT-6484879.
STRINGi192222.Cj0855.

Proteomic databases

PaxDbiQ0PA35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34983; CAL34983; Cj0855.
GeneIDi905155.
KEGGicje:Cj0855.
PATRICi20058658. VBICamJej33762_0843.

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciCJEJ192222:GJTS-796-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ0PA35.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOLD_CAMJE
AccessioniPrimary (citable) accession number: Q0PA35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 19, 2006
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.