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Protein

Undecaprenyl-diphosphooligosaccharide--protein glycotransferase

Gene

pglB

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Oligosaccharyltransferase that catalyzes the transfer of a preassembled heptasaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains, affording a beta-linked glycan to the asparagine side chain of target proteins.1 Publication

Catalytic activityi

Tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine = tritrans,heptacis-undecaprenyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei54 – 541By similarity
Metal bindingi54 – 541Magnesium 1By similarity
Metal bindingi71 – 711Magnesium 2By similarity
Metal bindingi74 – 741Magnesium 2By similarity
Binding sitei150 – 1501Target acceptor peptideBy similarity
Active sitei152 – 1521By similarity
Metal bindingi152 – 1521Magnesium 1By similarity
Active sitei316 – 3161By similarity
Metal bindingi316 – 3161Magnesium 1By similarity
Binding sitei328 – 3281Target acceptor peptideBy similarity
Binding sitei459 – 4591Target acceptor peptideBy similarity
Metal bindingi469 – 4691Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

  • protein N-linked glycosylation via asparagine Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1099-MONOMER.
MetaCyc:MONOMER-17335.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

Names & Taxonomyi

Protein namesi
Recommended name:
Undecaprenyl-diphosphooligosaccharide--protein glycotransferase (EC:2.4.99.19)
Alternative name(s):
Protein glycosylation B
Gene namesi
Name:pglB
Ordered Locus Names:Cj1126c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321HelicalSequence analysisAdd
BLAST
Topological domaini34 – 9966PeriplasmicSequence analysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence analysisAdd
BLAST
Topological domaini121 – 1255CytoplasmicSequence analysis
Transmembranei126 – 14318HelicalSequence analysisAdd
BLAST
Topological domaini144 – 17633PeriplasmicSequence analysisAdd
BLAST
Transmembranei177 – 19317HelicalSequence analysisAdd
BLAST
Topological domaini194 – 1974CytoplasmicSequence analysis
Transmembranei198 – 21821HelicalSequence analysisAdd
BLAST
Topological domaini219 – 23214PeriplasmicSequence analysisAdd
BLAST
Transmembranei233 – 25321HelicalSequence analysisAdd
BLAST
Topological domaini254 – 2607CytoplasmicSequence analysis
Transmembranei261 – 28121HelicalSequence analysisAdd
BLAST
Topological domaini282 – 32847PeriplasmicSequence analysisAdd
BLAST
Transmembranei329 – 34921HelicalSequence analysisAdd
BLAST
Topological domaini350 – 3523CytoplasmicSequence analysis
Transmembranei353 – 37321HelicalSequence analysisAdd
BLAST
Topological domaini374 – 3763PeriplasmicSequence analysis
Transmembranei377 – 39721HelicalSequence analysisAdd
BLAST
Topological domaini398 – 4069CytoplasmicSequence analysis
Transmembranei407 – 42721HelicalSequence analysisAdd
BLAST
Topological domaini428 – 713286PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 713713Undecaprenyl-diphosphooligosaccharide--protein glycotransferasePRO_0000422587Add
BLAST

Proteomic databases

PaxDbiQ0P9C8.

Interactioni

Protein-protein interaction databases

IntActiQ0P9C8. 1 interaction.
STRINGi192222.Cj1126c.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni315 – 3162Target acceptor peptide bindingBy similarity

Sequence similaritiesi

Belongs to the STT3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107ZAZ. Bacteria.
COG1287. LUCA.
HOGENOMiHOG000103852.
KOiK17251.
OMAiGYPIRYY.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0P9C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKKEYLKNP YLVLFAMIVL AYVFSVFCRF YWVWWASEFN EYFFNNQLMI
60 70 80 90 100
ISNDGYAFAE GARDMIAGFH QPNDLSYYGS SLSTLTYWLY KITPFSFESI
110 120 130 140 150
ILYMSTFLSS LVVIPIILLA NEYKRPLMGF VAALLASVAN SYYNRTMSGY
160 170 180 190 200
YDTDMLVIVL PMFILFFMVR MILKKDFFSL IALPLFIGIY LWWYPSSYTL
210 220 230 240 250
NVALIGLFLI YTLIFHRKEK IFYIAVILSS LTLSNIAWFY QSAIIVILFA
260 270 280 290 300
LFALEQKRLN FMIIGILGSA TLIFLILSGG VDPILYQLKF YIFRSDESAN
310 320 330 340 350
LTQGFMYFNV NQTIQEVENV DFSEFMRRIS GSEIVFLFSL FGFVWLLRKH
360 370 380 390 400
KSMIMALPIL VLGFLALKGG LRFTIYSVPV MALGFGFLLS EFKAILVKKY
410 420 430 440 450
SQLTSNVCIV FATILTLAPV FIHIYNYKAP TVFSQNEASL LNQLKNIANR
460 470 480 490 500
EDYVVTWWDY GYPVRYYSDV KTLVDGGKHL GKDNFFPSFS LSKDEQAAAN
510 520 530 540 550
MARLSVEYTE KSFYAPQNDI LKSDILQAMM KDYNQSNVDL FLASLSKPDF
560 570 580 590 600
KIDTPKTRDI YLYMPARMSL IFSTVASFSF INLDTGVLDK PFTFSTAYPL
610 620 630 640 650
DVKNGEIYLS NGVVLSDDFR SFKIGDNVVS VNSIVEINSI KQGEYKITPI
660 670 680 690 700
DDKAQFYIFY LKDSAIPYAQ FILMDKTMFN SAYVQMFFLG NYDKNLFDLV
710
INSRDAKVFK LKI
Length:713
Mass (Da):82,201
Last modified:September 19, 2006 - v1
Checksum:i0ACC149A14968DE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35243.1.
PIRiA81317.
RefSeqiWP_002852780.1. NC_002163.1.
YP_002344519.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35243; CAL35243; Cj1126c.
GeneIDi905417.
KEGGicje:Cj1126c.
PATRICi20059210. VBICamJej33762_1108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35243.1.
PIRiA81317.
RefSeqiWP_002852780.1. NC_002163.1.
YP_002344519.1. NC_002163.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ0P9C8. 1 interaction.
STRINGi192222.Cj1126c.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

Proteomic databases

PaxDbiQ0P9C8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL35243; CAL35243; Cj1126c.
GeneIDi905417.
KEGGicje:Cj1126c.
PATRICi20059210. VBICamJej33762_1108.

Phylogenomic databases

eggNOGiENOG4107ZAZ. Bacteria.
COG1287. LUCA.
HOGENOMiHOG000103852.
KOiK17251.
OMAiGYPIRYY.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciCJEJ192222:GJTS-1099-MONOMER.
MetaCyc:MONOMER-17335.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLB_CAMJE
AccessioniPrimary (citable) accession number: Q0P9C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: September 19, 2006
Last modified: September 7, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

N-linked protein glycosylation in C.jejuni consists in the transfer of a heptasaccharide (GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-bacillosamine) from a membrane-anchored undecaprenylpyrophosphate (Und-PP)-linked donor to the Asn side chain of proteins at the Asn-X-Ser/Thr motif.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.