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Protein

UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase

Gene

pseG

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleotide sugar hydrolase that catalyzes the fourth step in the biosynthesis of pseudaminic acid, a sialic-acid-like sugar that is used to modify flagellin. Mediates the removal of UDP from C-1 of UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose forming 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose.1 Publication

Catalytic activityi

UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O = 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + UDP.1 Publication

Kineticsi

kcat is 27 sec(-1) (PubMed:16728396). Note=kcat is 25 sec(-1) (PubMed:19483088).2 Publications

Manual assertion based on experiment ini

  1. KM=174 µM for UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose2 Publications
  2. KM=250 µM for UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei17Proton acceptor1 Publication1
    Binding sitei143Substrate1
    Binding sitei239Substrate1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciCJEJ192222:GJTS-1239-MONOMER.
    BRENDAi3.6.1.57. 1087.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase (EC:3.6.1.57)
    Alternative name(s):
    Pseudaminic acid biosynthesis protein G
    Gene namesi
    Name:pseG
    Ordered Locus Names:Cj1312
    OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
    Taxonomic identifieri192222 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    Proteomesi
    • UP000000799 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi17H → F or L: Abolishes catalytic activity. 1 Publication1
    Mutagenesisi17H → N: Strongly impaired catalytic activity. 1 Publication1
    Mutagenesisi78Y → F: Impaired catalytic activity. 1 Publication1
    Mutagenesisi255N → A: Impaired catalytic activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004189311 – 274UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolaseAdd BLAST274

    Proteomic databases

    PaxDbiQ0P8U5.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    IntActiQ0P8U5. 6 interactors.
    STRINGi192222.Cj1312.

    Structurei

    Secondary structure

    1274
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 6Combined sources4
    Turni11 – 13Combined sources3
    Helixi16 – 25Combined sources10
    Beta strandi29 – 35Combined sources7
    Helixi43 – 45Combined sources3
    Beta strandi50 – 52Combined sources3
    Beta strandi54 – 56Combined sources3
    Helixi58 – 68Combined sources11
    Beta strandi71 – 75Combined sources5
    Helixi82 – 92Combined sources11
    Beta strandi95 – 99Combined sources5
    Beta strandi109 – 113Combined sources5
    Helixi120 – 123Combined sources4
    Turni124 – 126Combined sources3
    Beta strandi132 – 136Combined sources5
    Helixi137 – 139Combined sources3
    Helixi144 – 149Combined sources6
    Beta strandi158 – 163Combined sources6
    Beta strandi165 – 167Combined sources3
    Helixi172 – 179Combined sources8
    Beta strandi186 – 190Combined sources5
    Helixi197 – 205Combined sources9
    Beta strandi208 – 215Combined sources8
    Helixi219 – 224Combined sources6
    Beta strandi226 – 234Combined sources9
    Helixi235 – 242Combined sources8
    Beta strandi247 – 250Combined sources4
    Helixi254 – 256Combined sources3
    Helixi257 – 265Combined sources9
    Beta strandi269 – 271Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3HBMX-ray1.80A1-274[»]
    3HBNX-ray1.85A1-274[»]
    SMRiQ0P8U5.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ0P8U5.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni15 – 16Substrate binding2
    Regioni234 – 235Substrate binding2

    Sequence similaritiesi

    Belongs to the PseG family.Curated

    Phylogenomic databases

    eggNOGiENOG4107DKY. Bacteria.
    COG3980. LUCA.
    HOGENOMiHOG000229436.
    KOiK15897.
    OMAiYEVEYRY.

    Family and domain databases

    InterProiIPR020023. PseG.
    [Graphical view]
    TIGRFAMsiTIGR03590. PseG. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0P8U5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKVLFRSDSS SQIGFGHIKR DLVLAKQYSD VSFACLPLEG SLIDEIPYPV
    60 70 80 90 100
    YELSSESIYE LINLIKEEKF ELLIIDHYGI SVDDEKLIKL ETGVKILSFD
    110 120 130 140 150
    DEIKPHHCDI LLNVNAYAKA SDYEGLVPFK CEVRCGFSYA LIREEFYQEA
    160 170 180 190 200
    KENREKKYDF FICMGGTDIK NLSLQIASEL PKTKIISIAT SSSNPNLKKL
    210 220 230 240 250
    QKFAKLHNNI RLFIDHENIA KLMNESNKLI ISASSLVNEA LLLKANFKAI
    260 270
    CYVKNQESTA TWLAKKGYEV EYKY
    Length:274
    Mass (Da):31,319
    Last modified:September 19, 2006 - v1
    Checksum:iA65BF88D58502257
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL111168 Genomic DNA. Translation: CAL35426.1.
    PIRiE81274.
    RefSeqiWP_002830499.1. NC_002163.1.
    YP_002344702.1. NC_002163.1.

    Genome annotation databases

    EnsemblBacteriaiCAL35426; CAL35426; Cj1312.
    GeneIDi905604.
    KEGGicje:Cj1312.
    PATRICi20059588. VBICamJej33762_1294.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL111168 Genomic DNA. Translation: CAL35426.1.
    PIRiE81274.
    RefSeqiWP_002830499.1. NC_002163.1.
    YP_002344702.1. NC_002163.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3HBMX-ray1.80A1-274[»]
    3HBNX-ray1.85A1-274[»]
    SMRiQ0P8U5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ0P8U5. 6 interactors.
    STRINGi192222.Cj1312.

    Proteomic databases

    PaxDbiQ0P8U5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAL35426; CAL35426; Cj1312.
    GeneIDi905604.
    KEGGicje:Cj1312.
    PATRICi20059588. VBICamJej33762_1294.

    Phylogenomic databases

    eggNOGiENOG4107DKY. Bacteria.
    COG3980. LUCA.
    HOGENOMiHOG000229436.
    KOiK15897.
    OMAiYEVEYRY.

    Enzyme and pathway databases

    BioCyciCJEJ192222:GJTS-1239-MONOMER.
    BRENDAi3.6.1.57. 1087.

    Miscellaneous databases

    EvolutionaryTraceiQ0P8U5.

    Family and domain databases

    InterProiIPR020023. PseG.
    [Graphical view]
    TIGRFAMsiTIGR03590. PseG. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPSEG_CAMJE
    AccessioniPrimary (citable) accession number: Q0P8U5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: September 19, 2006
    Last modified: November 2, 2016
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.