Reviewed, UniProtKB/Swiss-Prot Q0P8G1 (RIMO_CAMJE)

Names and origin

Protein names

Recommended name:
    Ribosomal protein S12 methylthiotransferase rimO
      Short name=S12 methylthiotransferase
      Short name=S12 MTTase
    EC=2.-.-.-
Alternative name(s):
    Ribosome maturation factor rimO

Gene names

Name:	rimO
Ordered Locus Names:	Cj1454c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 By similarity.
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methylthiotransferase family. RimO subfamily. Contains 1 MTTase N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	catalytic activity Inferred from electronic annotation. Source: InterPro iron-sulfur cluster binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
flaG	Q0PAW9	1	EBI-1195362,EBI-1191365

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Ribosomal protein S12 methylthiotransferase rimO		PRO_0000374753
Regions
	Domain	2 – 114	113	MTTase N-terminal
Sites
	Metal binding	11	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	45	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	77	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	146	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	150	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	153	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0P8G1-1 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: 0A6D71ABAA386ADD	FASTA	439	49,861
	10 20 30 40 50 60 MSKLYLMSLG CNKNLVDSEI MLGHLSAYEL CDEPSKADVL IVNTCGFIDS AKKESINAIL 70 80 90 100 110 120 DLHEQRKKDS LLVVTGCLMQ RYREELMKEL PEVDLFTGVG DYERIDEMIL KKTNLFSNST 130 140 150 160 170 180 YLQSENSKRI ITGSNSHAFI KIAEGCNQKC SFCAIPSFKG KLKSREISSI IAELKDLVAR 190 200 210 220 230 240 GYKDFSFIAQ DTSSYLFDKG EKDGLIRLID EVEKIKGIRA ARILYLYPTS ASEALIKRII 250 260 270 280 290 300 ASEIFINYFD MPLQHISDNM LKIMKRGANS TRLKEMLNLM KSAPNSFLRT GFIVGHPGES 310 320 330 340 350 360 EADFEELCEF VKDFGFDRIS VFAYSKEEDT AAFDMEQVPF KVINKRLKII EKIVDEVIEK 370 380 390 400 410 420 SFEKEVGQKR LVVCTGKSSE GEFFIAAKDL RWDREIDGEI LINESECGNL EMGQIYECEI 430 LQNLDKKLLA KALRKVDAN

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35562.1.
PIR	E81291.
RefSeq	YP_002344836.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q0P8G1. 48 interactions.
Genome annotation databases
GeneID	905742.
GenomeReviews	Gene locus Cj1454c in contig AL111168_GR.
KEGG	cje:Cj1454c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0P8G1.
OMA	Q0P8G1. LGAWVRL.
Family and domain databases
HAMAP	MF_01865. [Tree]
InterPro	IPR005840. CHP01125. IPR006638. Elp3/MiaB/NifB. IPR007197. Radical_SAM. IPR005839. UPF0004. IPR013848. UPF0004_N. [Graphical view]
PANTHER	PTHR11918. UPF0004. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01125. CHP1125. 1 hit. TIGR00089. UPF0004. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIMO_CAMJE

Accession

Primary (citable) accession number: Q0P8G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	September 19, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)