Q0P891 (DPS_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA protection during starvation protein
EC=1.16.-.-

Gene names

Name:	dps
Ordered Locus Names:	Cj1534c

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protects DNA from oxidative damage by sequestering intracellular Fe2+ ion and storing it in the form of Fe3+ oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe2+ ions, which prevents hydroxyl radical production by the Fenton reaction Probable. Does not bind DNA. Ref.1
Catalytic activity	2 Fe2+ + H2O2 + 2 H+ = 2 Fe3+ + 2 H2O.
Subunit structure	Homododecamer. The 12 subunits form a hollow sphere into which the mineral iron core of up to 500 Fe3+ can be deposited By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	Constitutively expressed. Not induced by hydrogen peroxide or by iron.
Sequence similarities	Belongs to the dps family.

Ontologies

Keywords
Biological process	Iron storage
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferric iron binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliY	Q0PC74	1	EBI-1192444,EBI-1191111

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 149

149

DNA protection during starvation protein

PRO_0000253339

Sites

Metal binding

25

1

Iron 1; shared with dodecameric partner By similarity

Metal binding

52

1

Iron 1 By similarity

Metal binding

56

1

Iron 1 By similarity

Metal binding

56

1

Iron 2 By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

149

Helix Strand Turn

Details...

Helix

3 – 27

25

Helix

33 – 60

28

Helix

69 – 75

7

Helix

88 – 115

28

Helix

119 – 138

20

Sequences

	Sequence		Length	Mass (Da)
	Q0P891 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: 203EB8DE513D0884	FASTA	149	17,205
	10 20 30 40 50 60 MSVTKQLLQM QADAHHLWVK FHNYHWNVKG LQFFSIHEYT EKAYEEMAEL FDSCAERVLQ 70 80 90 100 110 120 LGEKAITCQK VLMENAKSPK VAKDCFTPLE VIELIKQDYE YLLAEFKKLN EAAEKESDTT 130 140 TAAFAQENIA KYEKSLWMIG ATLQGACKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The iron-binding protein Dps confers hydrogen peroxide stress resistance to Campylobacter jejuni." Ishikawa T., Mizunoe Y., Kawabata S., Takade A., Harada M., Wai S.N., Yoshida S. J. Bacteriol. 185:1010-1017(2003) [PubMed: 12533477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, IRON BINDING, FUNCTION IN PROTECTION OF DNA FROM HYDROGEN PEROXIDE STRESS.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL111168 Genomic DNA. Translation: CAL35634.1.

PIR

D81300.

RefSeq

YP_002344906.1. NC_002163.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3KWO	X-ray	1.98	A/B/C/D	1-149	[»]

ProteinModelPortal

Q0P891.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q0P891. 37 interactions.

PTM databases

PhosSite

Q0P891.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

905816.

GenomeReviews

Gene locus Cj1534c in contig AL111168_GR.

KEGG

cje:Cj1534c.

PATRIC

20060028. VBICamJej33762_1511.

Phylogenomic databases

HOGENOM

HBG672882.

OMA

NESAEQM.

PhylomeDB

Q0P891.

ProtClustDB

CLSK2767721.

Enzyme and pathway databases

BioCyc

CJEJ192222:CJ1534C-MONOMER.

Family and domain databases

InterPro

IPR002177. DPS_DNA-bd.
IPR012347. Ferritin-rel.
IPR009078. Ferritin/RR-like.
IPR008331. Ferritin_DPS_dom.
[Graphical view]

Gene3D

G3DSA:1.20.1260.10. Ferritin_rel. 1 hit.

KO

K04047.

Pfam

PF00210. Ferritin. 1 hit.
[Graphical view]

PIRSF

PIRSF005900. Dps. 1 hit.

PRINTS

PR01346. HELNAPAPROT.

SUPFAM

SSF47240. Ferritin/RR_like. 1 hit.

ProtoNet

Search...

Entry information

Entry name

DPS_CAMJE

Accession

Primary (citable) accession number: Q0P891

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	September 19, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program