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Protein

Vacuolar protein sorting-associated protein 8 homolog

Gene

Vps8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1257 – 1309RING-type; atypicalPROSITE-ProRule annotationAdd BLAST53

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 8 homolog
Gene namesi
Name:Vps8
Synonyms:Kiaa0804
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2146407. Vps8.

Subcellular locationi

GO - Cellular componenti

  • CORVET complex Source: UniProtKB
  • early endosome Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782681 – 1427Vacuolar protein sorting-associated protein 8 homologAdd BLAST1427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineBy similarity1
Modified residuei32PhosphoserineBy similarity1
Modified residuei127PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ0P5W1.
PaxDbiQ0P5W1.
PeptideAtlasiQ0P5W1.
PRIDEiQ0P5W1.

PTM databases

iPTMnetiQ0P5W1.
PhosphoSitePlusiQ0P5W1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033653.
ExpressionAtlasiQ0P5W1. baseline and differential.
GenevisibleiQ0P5W1. MM.

Interactioni

Subunit structurei

Interacts with RAB5C (PubMed:25266290). Interacts with TGFBRAP1 (By similarity). Component of the putative class C core vacuaole/endosome tethering (CORVET) complex; the core of which is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, associated with VPS8 and TGFBRAP1 (PubMed:25266290).By similarity1 Publication1 Publication

Protein-protein interaction databases

IntActiQ0P5W1. 1 interactor.
STRINGi10090.ENSMUSP00000111055.

Structurei

3D structure databases

ProteinModelPortaliQ0P5W1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati193 – 234WDAdd BLAST42

Sequence similaritiesi

Belongs to the VPS8 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 WD repeat.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1257 – 1309RING-type; atypicalPROSITE-ProRule annotationAdd BLAST53

Keywords - Domaini

WD repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2079. Eukaryota.
ENOG410XRX6. LUCA.
GeneTreeiENSGT00390000010672.
HOGENOMiHOG000013171.
HOVERGENiHBG071091.
InParanoidiQ0P5W1.
KOiK20178.
TreeFamiTF314244.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR025941. Vps8_central_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12816. Vps8. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q0P5W1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METEPDQEHL DQNPCARTVE EELSKSFNLE ASLSKFSCLD LDKELEFRSD
60 70 80 90 100
LIDDKEFDIP QVDTPPTLES ILNETDDEDE SFVLEDPTLL NVDTIDSHSY
110 120 130 140 150
DTSSVASSDS GDRANLKRKK KLPDSFSLHG SVMRHSLLKG ISAQIVSAAD
160 170 180 190 200
KVDAGLPTAI AVSSLIAVGT SHGLALIFDQ NQALRLCLGS TSVGGQYGAI
210 220 230 240 250
SALSINNDCS RLLCGFAKGQ ITMWDLASGK LLRSITDAHP PGTAILHIKF
260 270 280 290 300
TDDPTLAICN DSGGSVFELT FKRVMGVRTC ESRCLFSGSK GEVCCIEPLH
310 320 330 340 350
SKPELKDHPI TQFSLLAMAS LTKILVIGLK PSLKVWMTFP YGRMDPSSVP
360 370 380 390 400
LLAWHFVAVN NSVNPMLAFC RGDMVHFLLV KRDESGAIHV TKQKHLHLYY
410 420 430 440 450
DLINFTWINS RTVVLLDSVE KLHVIDRQTQ EELETMEISE VQLVYNSSHF
460 470 480 490 500
KSLATGGNVS QALALVGEKA CYQSISSYGG QIFYLGTKSV YVMMLRSWRE
510 520 530 540 550
RMDHLLKQDC LTEALALAWS FHEGKAKAVV GLSGDVSKRK AVVADRMVEI
560 570 580 590 600
LFHYADRALK KCPDQGKIQV MEQHFQDTVP VIVDYCLLLQ RKDLLFGQMY
610 620 630 640 650
DKLSENSVAK GVFLECLEPY ILSDKLVGIT PQVMKDLIVH FQDKKLLENV
660 670 680 690 700
EALIVHMDIT SLDIQQVVLM CWENRLYDAM VYVYNRGMNE FISPMEKLFK
710 720 730 740 750
VIAPPLNAGK TLTDEQVVMG NKLLVYISCC LAGRAYPLGD IPEDLVPLVK
760 770 780 790 800
NQVFEFLIRL HSVEASSEEE VYPYVRTLLH FDTREFLNVL ALTFEDFKND
810 820 830 840 850
KQAVEYQQRI VDILLKVMVE NSDFTPSQVG CLFTFLARQL AKPDNTLFVN
860 870 880 890 900
RTLFDQVLEF LCSPDDDSRH SERQQVLLEL LQAGGIVQFE ESRLIRMAEK
910 920 930 940 950
AEFYQICEFM YEREHQYDKI IDCYLHDPLR EEEVFNYIHN ILSIPGHSAE
960 970 980 990 1000
EKQSVWQKAM NHMEELVSLK PCKAAELVAT HFSEQIEVVI GQLQNQLLLF
1010 1020 1030 1040 1050
KFLRSLLDPR EGVHVNQELL QIPPHITEQF IELLCQFSPD QVIQTLQVLE
1060 1070 1080 1090 1100
CYRLEETIQI TQKYQLHEVT AYLLEKKGDA HGAFLLLLER LQSRLQEMTR
1110 1120 1130 1140 1150
QDENTKEDIL LKGVEDTMVE TIALCQRNSQ NLNQQQREAL WFPLLEAMMT
1160 1170 1180 1190 1200
PQKLSSSAAA PHPHCEALKS LTMQVLNSMA AFIALPSILQ RILQDPIYGK
1210 1220 1230 1240 1250
GKLGEIQGLI LGMLDTFNYE QTLLETTASL LNQDLHWSLC NLRASVSRGL
1260 1270 1280 1290 1300
NPKQDYCSIC LQQYKRRQEM ADEIIVFSCG HLYHSFCLQS KECTLEVEGQ
1310 1320 1330 1340 1350
TRWACHKCSS SNKAGKLSEN PSENKKGRIT SSQVKMSPSY HQSKGDPPAR
1360 1370 1380 1390 1400
KANSEPVLDP QQMQAFDQLC RLYRGSSRLA LLTELSQNRG GDSCRPFAGP
1410 1420
QSGPAFNSVF QKENFQLQLA PPPVAED
Length:1,427
Mass (Da):161,146
Last modified:September 19, 2006 - v1
Checksum:i7E88538003B36B72
GO
Isoform 2 (identifier: Q0P5W1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1278-1292: SCGHLYHSFCLQSKE → RFLYWVTGASPIQPK
     1293-1427: Missing.

Note: No experimental confirmation available.
Show »
Length:1,292
Mass (Da):146,393
Checksum:iD47F5716E4CE0631
GO
Isoform 3 (identifier: Q0P5W1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-178: F → FGK
     965-994: Missing.

Note: No experimental confirmation available.
Show »
Length:1,399
Mass (Da):158,068
Checksum:i6C337962F31AB989
GO

Sequence cautioni

The sequence AAH32214 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC26237 differs from that shown. Reason: Frameshift at position 631.Curated
The sequence BAC65657 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti591R → G in BAC26237 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023252178F → FGK in isoform 3. 1 Publication1
Alternative sequenceiVSP_023253965 – 994Missing in isoform 3. 1 PublicationAdd BLAST30
Alternative sequenceiVSP_0232541278 – 1292SCGHL…LQSKE → RFLYWVTGASPIQPK in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_0232551293 – 1427Missing in isoform 2. 1 PublicationAdd BLAST135

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122375 mRNA. Translation: BAC65657.1. Different initiation.
AK029007 mRNA. Translation: BAC26237.1. Frameshift.
AK141678 mRNA. Translation: BAE24795.1.
BC032214 mRNA. Translation: AAH32214.1. Different initiation.
BC055323 mRNA. Translation: AAH55323.1.
CCDSiCCDS70700.1. [Q0P5W1-3]
CCDS70701.1. [Q0P5W1-1]
RefSeqiNP_001272822.1. NM_001285893.1. [Q0P5W1-1]
NP_001272823.1. NM_001285894.1. [Q0P5W1-3]
UniGeneiMm.235683.

Genome annotation databases

EnsembliENSMUST00000096191; ENSMUSP00000093905; ENSMUSG00000033653. [Q0P5W1-2]
ENSMUST00000117598; ENSMUSP00000112937; ENSMUSG00000033653. [Q0P5W1-1]
ENSMUST00000118923; ENSMUSP00000112636; ENSMUSG00000033653. [Q0P5W1-3]
GeneIDi209018.
KEGGimmu:209018.
UCSCiuc007yrj.2. mouse. [Q0P5W1-1]
uc012adi.2. mouse. [Q0P5W1-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122375 mRNA. Translation: BAC65657.1. Different initiation.
AK029007 mRNA. Translation: BAC26237.1. Frameshift.
AK141678 mRNA. Translation: BAE24795.1.
BC032214 mRNA. Translation: AAH32214.1. Different initiation.
BC055323 mRNA. Translation: AAH55323.1.
CCDSiCCDS70700.1. [Q0P5W1-3]
CCDS70701.1. [Q0P5W1-1]
RefSeqiNP_001272822.1. NM_001285893.1. [Q0P5W1-1]
NP_001272823.1. NM_001285894.1. [Q0P5W1-3]
UniGeneiMm.235683.

3D structure databases

ProteinModelPortaliQ0P5W1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ0P5W1. 1 interactor.
STRINGi10090.ENSMUSP00000111055.

PTM databases

iPTMnetiQ0P5W1.
PhosphoSitePlusiQ0P5W1.

Proteomic databases

MaxQBiQ0P5W1.
PaxDbiQ0P5W1.
PeptideAtlasiQ0P5W1.
PRIDEiQ0P5W1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000096191; ENSMUSP00000093905; ENSMUSG00000033653. [Q0P5W1-2]
ENSMUST00000117598; ENSMUSP00000112937; ENSMUSG00000033653. [Q0P5W1-1]
ENSMUST00000118923; ENSMUSP00000112636; ENSMUSG00000033653. [Q0P5W1-3]
GeneIDi209018.
KEGGimmu:209018.
UCSCiuc007yrj.2. mouse. [Q0P5W1-1]
uc012adi.2. mouse. [Q0P5W1-3]

Organism-specific databases

CTDi23355.
MGIiMGI:2146407. Vps8.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2079. Eukaryota.
ENOG410XRX6. LUCA.
GeneTreeiENSGT00390000010672.
HOGENOMiHOG000013171.
HOVERGENiHBG071091.
InParanoidiQ0P5W1.
KOiK20178.
TreeFamiTF314244.

Miscellaneous databases

ChiTaRSiVps8. mouse.
PROiQ0P5W1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033653.
ExpressionAtlasiQ0P5W1. baseline and differential.
GenevisibleiQ0P5W1. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR025941. Vps8_central_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12816. Vps8. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVPS8_MOUSE
AccessioniPrimary (citable) accession number: Q0P5W1
Secondary accession number(s): Q3UR93, Q80TR5, Q8K280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: September 19, 2006
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.