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Q0P5I5 (HOGA1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxy-2-oxoglutarate aldolase, mitochondrial

EC=4.1.3.16
Alternative name(s):
Dihydrodipicolinate synthase-like
Short name=DHDPS-like protein
Probable 2-keto-4-hydroxyglutarate aldolase
Short name=Probable KHG-aldolase
Gene names
Name:HOGA1
Synonyms:DHDPSL
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the final step in the metabolic pathway of hydroxyproline By similarity.

Catalytic activity

4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.

Enzyme regulation

Inhibited by divalent cations.

Subunit structure

Homotetramer. Ref.4

Subcellular location

Mitochondrion Ref.3.

Sequence similarities

Belongs to the DapA family.

Biophysicochemical properties

Kinetic parameters:

KM=26 µM for DL-4-hydroxy-2-oxoglutarate Ref.4

Vmax=10.7 µmol/min/mg enzyme

pH dependence:

Optimum pH is 8.8.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2525Mitochondrion Ref.3
Chain26 – 3273024-hydroxy-2-oxoglutarate aldolase, mitochondrial
PRO_0000273345

Regions

Region77 – 782Substrate binding By similarity

Sites

Active site1961Schiff-base intermediate with substrate By similarity
Binding site1981Substrate By similarity
Binding site2221Substrate; via carbonyl oxygen By similarity
Site1681Involved in proton transfer during cleavage By similarity

Experimental info

Sequence conflict3271L → F in ABH06334. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q0P5I5 [UniParc].

Last modified September 19, 2006. Version 1.
Checksum: 153314DCD1F0D587

FASTA32735,217
        10         20         30         40         50         60 
MLVPRVWSSV RLGLSRVLSR TLRGWPSGEG RGMDLSGIYP PVTTPFTATA EVDYGKLEEN 

        70         80         90        100        110        120 
LHKLGTLPFR GFVVQGSNGE FPFLTSSERL EVVSRARQAL PKDKLLLAGS GCESTQATVE 

       130        140        150        160        170        180 
MTVSMAQVGA DAAMVVTPCY YRGRMSSAAL IHHYTKVADL SPVPVVLYSV PANTGLDLPV 

       190        200        210        220        230        240 
DAVVTLSQHP NIVGIKDSGG DVTRIGLIVH KTRSQDFQVL AGSAGFLLAS YAIGAVGGVC 

       250        260        270        280        290        300 
ALANVLGSQV CQLERLCLTG QWEDAQKLQH RLIEPNTAVT RRFGIPGLKK TMDWFGYYGG 

       310        320 
PCRSPLQELS PAQEEALRLD FASNGWL 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal liver.
[3]"Structural and biochemical studies of human 4-hydroxy-2-oxoglutarate aldolase: implications for hydroxyproline metabolism in primary hyperoxaluria."
Riedel T.J., Johnson L.C., Knight J., Hantgan R.R., Holmes R.P., Lowther W.T.
PLoS ONE 6:E26021-E26021(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-30, TRANSIT PEPTIDE CLEAVAGE SITE, SUBCELLULAR LOCATION.
[4]"2-Keto-4-hydroxyglutarate aldolase: purification and characterization of the homogeneous enzyme from bovine kidney."
Dekker E.E., Kitson R.P.
J. Biol. Chem. 267:10507-10514(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
[5]"Mutations in DHDPSL are responsible for primary hyperoxaluria type III."
Belostotsky R., Seboun E., Idelson G.H., Milliner D.S., Becker-Cohen R., Rinat C., Monico C.G., Feinstein S., Ben-Shalom E., Magen D., Weissman I., Charon C., Frishberg Y.
Am. J. Hum. Genet. 87:392-399(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT026547 mRNA. Translation: ABH06334.1.
BC119998 mRNA. Translation: AAI19999.1.
RefSeqNP_001068705.1. NM_001075237.1.
UniGeneBt.54337.

3D structure databases

ProteinModelPortalQ0P5I5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000016909.

Proteomic databases

PRIDEQ0P5I5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000016909; ENSBTAP00000016909; ENSBTAG00000012721.
GeneID506001.
KEGGbta:506001.

Organism-specific databases

CTD112817.

Phylogenomic databases

eggNOGCOG0329.
GeneTreeENSGT00530000063604.
HOVERGENHBG081405.
InParanoidQ0P5I5.
OMATETCVDE.
OrthoDBEOG7H4DTV.
TreeFamTF324600.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
[Graphical view]
PANTHERPTHR12128. PTHR12128. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
PROSITEPS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20867406.

Entry information

Entry nameHOGA1_BOVIN
AccessionPrimary (citable) accession number: Q0P5I5
Secondary accession number(s): Q0V7M3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: September 19, 2006
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families