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Q0P5I5

- HOGA1_BOVIN

UniProt

Q0P5I5 - HOGA1_BOVIN

Protein

4-hydroxy-2-oxoglutarate aldolase, mitochondrial

Gene

HOGA1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (19 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the final step in the metabolic pathway of hydroxyproline.By similarity

    Catalytic activityi

    4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.

    Enzyme regulationi

    Inhibited by divalent cations.

    Kineticsi

    1. KM=26 µM for DL-4-hydroxy-2-oxoglutarate1 Publication

    Vmax=10.7 µmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 8.8.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei168 – 1681Involved in proton transfer during cleavageBy similarity
    Active sitei196 – 1961Schiff-base intermediate with substrateBy similarity
    Binding sitei198 – 1981SubstrateBy similarity
    Binding sitei222 – 2221Substrate; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. 4-hydroxy-2-oxoglutarate aldolase activity Source: UniProtKB

    GO - Biological processi

    1. 4-hydroxyproline catabolic process Source: Ensembl
    2. glyoxylate catabolic process Source: UniProtKB
    3. oxalate metabolic process Source: Ensembl
    4. pyruvate biosynthetic process Source: Ensembl

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Schiff base

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-2-oxoglutarate aldolase, mitochondrial (EC:4.1.3.16)
    Alternative name(s):
    Dihydrodipicolinate synthase-like
    Short name:
    DHDPS-like protein
    Probable 2-keto-4-hydroxyglutarate aldolase
    Short name:
    Probable KHG-aldolase
    Gene namesi
    Name:HOGA1
    Synonyms:DHDPSL
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 26

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: BHF-UCL

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2525Mitochondrion1 PublicationAdd
    BLAST
    Chaini26 – 3273024-hydroxy-2-oxoglutarate aldolase, mitochondrialPRO_0000273345Add
    BLAST

    Proteomic databases

    PRIDEiQ0P5I5.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000016909.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0P5I5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni77 – 782Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DapA family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0329.
    GeneTreeiENSGT00530000063604.
    HOVERGENiHBG081405.
    InParanoidiQ0P5I5.
    KOiK18123.
    OMAiCVTGMGN.
    OrthoDBiEOG7H4DTV.
    TreeFamiTF324600.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.
    PROSITEiPS00666. DHDPS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q0P5I5-1 [UniParc]FASTAAdd to Basket

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    MLVPRVWSSV RLGLSRVLSR TLRGWPSGEG RGMDLSGIYP PVTTPFTATA    50
    EVDYGKLEEN LHKLGTLPFR GFVVQGSNGE FPFLTSSERL EVVSRARQAL 100
    PKDKLLLAGS GCESTQATVE MTVSMAQVGA DAAMVVTPCY YRGRMSSAAL 150
    IHHYTKVADL SPVPVVLYSV PANTGLDLPV DAVVTLSQHP NIVGIKDSGG 200
    DVTRIGLIVH KTRSQDFQVL AGSAGFLLAS YAIGAVGGVC ALANVLGSQV 250
    CQLERLCLTG QWEDAQKLQH RLIEPNTAVT RRFGIPGLKK TMDWFGYYGG 300
    PCRSPLQELS PAQEEALRLD FASNGWL 327
    Length:327
    Mass (Da):35,217
    Last modified:September 19, 2006 - v1
    Checksum:i153314DCD1F0D587
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti327 – 3271L → F in ABH06334. (PubMed:16305752)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT026547 mRNA. Translation: ABH06334.1.
    BC119998 mRNA. Translation: AAI19999.1.
    RefSeqiNP_001068705.1. NM_001075237.1.
    UniGeneiBt.54337.

    Genome annotation databases

    EnsembliENSBTAT00000016909; ENSBTAP00000016909; ENSBTAG00000012721.
    GeneIDi506001.
    KEGGibta:506001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT026547 mRNA. Translation: ABH06334.1 .
    BC119998 mRNA. Translation: AAI19999.1 .
    RefSeqi NP_001068705.1. NM_001075237.1.
    UniGenei Bt.54337.

    3D structure databases

    ProteinModelPortali Q0P5I5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000016909.

    Proteomic databases

    PRIDEi Q0P5I5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000016909 ; ENSBTAP00000016909 ; ENSBTAG00000012721 .
    GeneIDi 506001.
    KEGGi bta:506001.

    Organism-specific databases

    CTDi 112817.

    Phylogenomic databases

    eggNOGi COG0329.
    GeneTreei ENSGT00530000063604.
    HOVERGENi HBG081405.
    InParanoidi Q0P5I5.
    KOi K18123.
    OMAi CVTGMGN.
    OrthoDBi EOG7H4DTV.
    TreeFami TF324600.

    Miscellaneous databases

    NextBioi 20867406.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    PROSITEi PS00666. DHDPS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal liver.
    3. "Structural and biochemical studies of human 4-hydroxy-2-oxoglutarate aldolase: implications for hydroxyproline metabolism in primary hyperoxaluria."
      Riedel T.J., Johnson L.C., Knight J., Hantgan R.R., Holmes R.P., Lowther W.T.
      PLoS ONE 6:E26021-E26021(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 26-30, TRANSIT PEPTIDE CLEAVAGE SITE, SUBCELLULAR LOCATION.
    4. "2-Keto-4-hydroxyglutarate aldolase: purification and characterization of the homogeneous enzyme from bovine kidney."
      Dekker E.E., Kitson R.P.
      J. Biol. Chem. 267:10507-10514(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
    5. Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiHOGA1_BOVIN
    AccessioniPrimary (citable) accession number: Q0P5I5
    Secondary accession number(s): Q0V7M3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: September 19, 2006
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3