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Protein

4-hydroxy-2-oxoglutarate aldolase, mitochondrial

Gene

HOGA1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the final step in the metabolic pathway of hydroxyproline.By similarity

Catalytic activityi

4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.

Enzyme regulationi

Inhibited by divalent cations.

Kineticsi

  1. KM=26 µM for DL-4-hydroxy-2-oxoglutarate1 Publication

Vmax=10.7 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.8.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei168 – 1681Involved in proton transfer during cleavageBy similarity
Active sitei196 – 1961Schiff-base intermediate with substrateBy similarity
Binding sitei198 – 1981SubstrateBy similarity
Binding sitei222 – 2221Substrate; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. 4-hydroxy-2-oxoglutarate aldolase activity Source: UniProtKB

GO - Biological processi

  1. 4-hydroxyproline catabolic process Source: Ensembl
  2. glyoxylate catabolic process Source: UniProtKB
  3. oxalate metabolic process Source: Ensembl
  4. pyruvate biosynthetic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Schiff base

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-2-oxoglutarate aldolase, mitochondrial (EC:4.1.3.16)
Alternative name(s):
Dihydrodipicolinate synthase-like
Short name:
DHDPS-like protein
Probable 2-keto-4-hydroxyglutarate aldolase
Short name:
Probable KHG-aldolase
Gene namesi
Name:HOGA1
Synonyms:DHDPSL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 26

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. mitochondrion Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2525Mitochondrion1 PublicationAdd
BLAST
Chaini26 – 3273024-hydroxy-2-oxoglutarate aldolase, mitochondrialPRO_0000273345Add
BLAST

Proteomic databases

PRIDEiQ0P5I5.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000016909.

Structurei

3D structure databases

ProteinModelPortaliQ0P5I5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni77 – 782Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DapA family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0329.
GeneTreeiENSGT00530000063604.
HOVERGENiHBG081405.
InParanoidiQ0P5I5.
KOiK18123.
OMAiICPSITI.
OrthoDBiEOG7H4DTV.
TreeFamiTF324600.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
PROSITEiPS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0P5I5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLVPRVWSSV RLGLSRVLSR TLRGWPSGEG RGMDLSGIYP PVTTPFTATA
60 70 80 90 100
EVDYGKLEEN LHKLGTLPFR GFVVQGSNGE FPFLTSSERL EVVSRARQAL
110 120 130 140 150
PKDKLLLAGS GCESTQATVE MTVSMAQVGA DAAMVVTPCY YRGRMSSAAL
160 170 180 190 200
IHHYTKVADL SPVPVVLYSV PANTGLDLPV DAVVTLSQHP NIVGIKDSGG
210 220 230 240 250
DVTRIGLIVH KTRSQDFQVL AGSAGFLLAS YAIGAVGGVC ALANVLGSQV
260 270 280 290 300
CQLERLCLTG QWEDAQKLQH RLIEPNTAVT RRFGIPGLKK TMDWFGYYGG
310 320
PCRSPLQELS PAQEEALRLD FASNGWL
Length:327
Mass (Da):35,217
Last modified:September 19, 2006 - v1
Checksum:i153314DCD1F0D587
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti327 – 3271L → F in ABH06334. (PubMed:16305752)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026547 mRNA. Translation: ABH06334.1.
BC119998 mRNA. Translation: AAI19999.1.
RefSeqiNP_001068705.1. NM_001075237.1.
UniGeneiBt.54337.

Genome annotation databases

EnsembliENSBTAT00000016909; ENSBTAP00000016909; ENSBTAG00000012721.
GeneIDi506001.
KEGGibta:506001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026547 mRNA. Translation: ABH06334.1.
BC119998 mRNA. Translation: AAI19999.1.
RefSeqiNP_001068705.1. NM_001075237.1.
UniGeneiBt.54337.

3D structure databases

ProteinModelPortaliQ0P5I5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000016909.

Proteomic databases

PRIDEiQ0P5I5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000016909; ENSBTAP00000016909; ENSBTAG00000012721.
GeneIDi506001.
KEGGibta:506001.

Organism-specific databases

CTDi112817.

Phylogenomic databases

eggNOGiCOG0329.
GeneTreeiENSGT00530000063604.
HOVERGENiHBG081405.
InParanoidiQ0P5I5.
KOiK18123.
OMAiICPSITI.
OrthoDBiEOG7H4DTV.
TreeFamiTF324600.

Miscellaneous databases

NextBioi20867406.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
PROSITEiPS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.
  3. "Structural and biochemical studies of human 4-hydroxy-2-oxoglutarate aldolase: implications for hydroxyproline metabolism in primary hyperoxaluria."
    Riedel T.J., Johnson L.C., Knight J., Hantgan R.R., Holmes R.P., Lowther W.T.
    PLoS ONE 6:E26021-E26021(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-30, TRANSIT PEPTIDE CLEAVAGE SITE, SUBCELLULAR LOCATION.
  4. "2-Keto-4-hydroxyglutarate aldolase: purification and characterization of the homogeneous enzyme from bovine kidney."
    Dekker E.E., Kitson R.P.
    J. Biol. Chem. 267:10507-10514(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
  5. Cited for: IDENTIFICATION.

Entry informationi

Entry nameiHOGA1_BOVIN
AccessioniPrimary (citable) accession number: Q0P5I5
Secondary accession number(s): Q0V7M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: September 19, 2006
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.