Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial precursor

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Q0P5I5 (HOGA1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 50. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial
EC=4.1.3.16

Alternative name(s):
Dihydrodipicolinate synthase-like
Short name=DHDPS-like protein
Probable 2-keto-4-hydroxyglutarate aldolase
Short name=Probable KHG-aldolase

Gene names

Name:	HOGA1
Synonyms:	DHDPSL

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the final step in the metabolic pathway of hydroxyproline By similarity.
Catalytic activity	4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.
Enzyme regulation	Inhibited by divalent cations.
Subunit structure	Homotetramer. Ref.3
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the DapA family.
Biophysicochemical properties	Kinetic parameters: K_M=26 µM for DL-4-hydroxy-2-oxoglutarate Ref.3 V_max=10.7 µmol/min/mg enzyme pH dependence: Optimum pH is 8.8.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	4-hydroxyproline catabolic process Inferred from electronic annotation. Source: Compara glyoxylate catabolic process Inferred from direct assay Ref.3. Source: UniProtKB oxalate metabolic process Inferred from electronic annotation. Source: Compara pyruvate biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrion Inferred from direct assay PubMed 21998747. Source: BHF-UCL
Molecular_function	4-hydroxy-2-oxoglutarate aldolase activity Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 25

Mitochondrion Potential

Chain

26 – 327

302

Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial

PRO_0000273345

Regions

Region

77 – 78

Substrate binding By similarity

Sites

Active site

196

Schiff-base intermediate with substrate By similarity

Site

168

Involved in proton transfer during cleavage By similarity

Experimental info

Sequence conflict

327

L → F in ABH06334. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q0P5I5 [UniParc].

Last modified September 19, 2006. Version 1.
Checksum: 153314DCD1F0D587
Show »

FASTA

327

35,217

        10         20         30         40         50         60 
MLVPRVWSSV RLGLSRVLSR TLRGWPSGEG RGMDLSGIYP PVTTPFTATA EVDYGKLEEN 

        70         80         90        100        110        120 
LHKLGTLPFR GFVVQGSNGE FPFLTSSERL EVVSRARQAL PKDKLLLAGS GCESTQATVE 

       130        140        150        160        170        180 
MTVSMAQVGA DAAMVVTPCY YRGRMSSAAL IHHYTKVADL SPVPVVLYSV PANTGLDLPV 

       190        200        210        220        230        240 
DAVVTLSQHP NIVGIKDSGG DVTRIGLIVH KTRSQDFQVL AGSAGFLLAS YAIGAVGGVC 

       250        260        270        280        290        300 
ALANVLGSQV CQLERLCLTG QWEDAQKLQH RLIEPNTAVT RRFGIPGLKK TMDWFGYYGG 

       310        320 
PCRSPLQELS PAQEEALRLD FASNGWL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.
[3]	"2-Keto-4-hydroxyglutarate aldolase: purification and characterization of the homogeneous enzyme from bovine kidney." Dekker E.E., Kitson R.P. J. Biol. Chem. 267:10507-10514(1992) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
[4]	"Mutations in DHDPSL are responsible for primary hyperoxaluria type III." Belostotsky R., Seboun E., Idelson G.H., Milliner D.S., Becker-Cohen R., Rinat C., Monico C.G., Feinstein S., Ben-Shalom E., Magen D., Weissman I., Charon C., Frishberg Y. Am. J. Hum. Genet. 87:392-399(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT026547 mRNA. Translation: ABH06334.1. BC119998 mRNA. Translation: AAI19999.1.
IPI	IPI00788592.
RefSeq	NP_001068705.1. NM_001075237.1.
UniGene	Bt.54337.
3D structure databases
ProteinModelPortal	Q0P5I5.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000016909.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000016909; ENSBTAP00000016909; ENSBTAG00000012721.
GeneID	506001.
KEGG	bta:506001.
Organism-specific databases
CTD	112817.
Phylogenomic databases
eggNOG	COG0329.
GeneTree	ENSGT00530000063604.
HOVERGEN	HBG081405.
InParanoid	Q0P5I5.
OMA	GCESTRA.
OrthoDB	EOG4NGGNF.
Enzyme and pathway databases
BioCyc	CATTLE:506001-MONOMER.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. [Graphical view]
PANTHER	PTHR12128. PTHR12128. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20867406.

Entry information

Entry name

HOGA1_BOVIN

Accession

Primary (citable) accession number: Q0P5I5
Secondary accession number(s): Q0V7M3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	September 19, 2006
Last modified:	May 29, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents