Reviewed, UniProtKB/Swiss-Prot Q0P5F0 (PPAL_BOVIN)

Names and origin

Protein names

Recommended name:
    Lysosomal acid phosphatase
      Short name=LAP
    EC=3.1.3.2

Gene names

Name:

ACP2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Lysosome membrane; Single-pass membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Note: The soluble form arises by proteolytic processing of the membrane-bound form By similarity.
Post-translational modification	The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Lysosome Membrane
Domain	Signal Transmembrane
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	By similarity
	Chain	31 – 423	393	Lysosomal acid phosphatase		PRO_0000352520
Regions
	Topological domain	31 – 380	350	Lumenal Potential
	Transmembrane	381 – 401	21	Potential
	Topological domain	402 – 423	22	Cytoplasmic Potential
Sites
	Active site	42	1	Nucleophile By similarity
	Active site	287	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	177	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Disulfide bond	159 ↔ 370		By similarity
	Disulfide bond	212 ↔ 310		By similarity
	Disulfide bond	345 ↔ 349		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0P5F0-1 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: A22B7E4B74CA57F6	FASTA	423	48,302
	10 20 30 40 50 60 MAGRRFGWSR AALLQLILGV NLMVMPRTQA RTLRFVTLLY RHGDRSPVKA YPKDPHQEDK 70 80 90 100 110 120 WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV YVRSTDFDRT LMSAEANLAG 130 140 150 160 170 180 LFPPDGIQRF NPNISWQPIP VHTVPVAEDR LLKFPLGPCP RFEQLQNETR RMPEYQNESV 190 200 210 220 230 240 QNAQFLDMVA NETGLTDLSL ETVWNVYDTL FCEQTHGLPL PPWASPQTMQ RLSRLKDFSF 250 260 270 280 290 300 RFLFGIYKQA EKARLQGGVL LAQIRKNLTL MATTSQLPKL LVYSAHDTTL VALHMALGVY 310 320 330 340 350 360 NGEQAPYASC HMFELYQEDS GNFSVEMYFR NESHRAPWPL TLPGCSHRCP LQDFLRLTEP 370 380 390 400 410 420 VVPKDWLQEC QLAGGPADTE VIVALAVCGS ILFLLIVLLL TVLFRVQAQP PGYRHVPDGE DHA

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pons.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC120138 mRNA. Translation: AAI20139.1.
IPI	IPI00717351.
RefSeq	NP_001069526.1.
UniGene	Bt.43294
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000027968; ENSBTAP00000027968; ENSBTAG00000021002; Bos taurus. [Genome view]
GeneID	535407.
KEGG	bta:535407.
Organism-specific databases
CTD	535407.
Phylogenomic databases
HOVERGEN	Q0P5F0.
OMA	ELYQEDS.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPAL_BOVIN

Accession

Primary (citable) accession number: Q0P5F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	September 19, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)