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Protein

Lysosomal acid phosphatase

Gene

ACP2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421NucleophileBy similarity
Active sitei287 – 2871Proton donorBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. lysosome organization Source: Ensembl
  2. skeletal system development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal acid phosphatase (EC:3.1.3.2)
Short name:
LAP
Gene namesi
Name:ACP2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 15

Subcellular locationi

  1. Lysosome membrane By similarity; Single-pass membrane protein By similarity; Lumenal side By similarity
  2. Lysosome lumen By similarity

  3. Note: The soluble form arises by proteolytic processing of the membrane-bound form.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini31 – 380350LumenalSequence AnalysisAdd
BLAST
Transmembranei381 – 40121HelicalSequence AnalysisAdd
BLAST
Topological domaini402 – 42322CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
  3. lysosomal lumen Source: UniProtKB-SubCell
  4. lysosomal membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030By similarityAdd
BLAST
Chaini31 – 423393Lysosomal acid phosphatasePRO_0000352520Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi159 ↔ 370By similarity
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi212 ↔ 310By similarity
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi345 ↔ 349By similarity

Post-translational modificationi

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ0P5F0.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027968.

Structurei

3D structure databases

ProteinModelPortaliQ0P5F0.
SMRiQ0P5F0. Positions 31-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG85977.
GeneTreeiENSGT00530000062956.
HOGENOMiHOG000231439.
HOVERGENiHBG002203.
InParanoidiQ0P5F0.
KOiK14410.
OMAiQDFLHLT.
OrthoDBiEOG7GXPBJ.
TreeFamiTF312893.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0P5F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRRFGWSR AALLQLILGV NLMVMPRTQA RTLRFVTLLY RHGDRSPVKA
60 70 80 90 100
YPKDPHQEDK WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV
110 120 130 140 150
YVRSTDFDRT LMSAEANLAG LFPPDGIQRF NPNISWQPIP VHTVPVAEDR
160 170 180 190 200
LLKFPLGPCP RFEQLQNETR RMPEYQNESV QNAQFLDMVA NETGLTDLSL
210 220 230 240 250
ETVWNVYDTL FCEQTHGLPL PPWASPQTMQ RLSRLKDFSF RFLFGIYKQA
260 270 280 290 300
EKARLQGGVL LAQIRKNLTL MATTSQLPKL LVYSAHDTTL VALHMALGVY
310 320 330 340 350
NGEQAPYASC HMFELYQEDS GNFSVEMYFR NESHRAPWPL TLPGCSHRCP
360 370 380 390 400
LQDFLRLTEP VVPKDWLQEC QLAGGPADTE VIVALAVCGS ILFLLIVLLL
410 420
TVLFRVQAQP PGYRHVPDGE DHA
Length:423
Mass (Da):48,302
Last modified:September 19, 2006 - v1
Checksum:iA22B7E4B74CA57F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120138 mRNA. Translation: AAI20139.1.
RefSeqiNP_001069526.1. NM_001076058.2.
UniGeneiBt.43294.

Genome annotation databases

EnsembliENSBTAT00000027968; ENSBTAP00000027968; ENSBTAG00000021002.
GeneIDi535407.
KEGGibta:535407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120138 mRNA. Translation: AAI20139.1.
RefSeqiNP_001069526.1. NM_001076058.2.
UniGeneiBt.43294.

3D structure databases

ProteinModelPortaliQ0P5F0.
SMRiQ0P5F0. Positions 31-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027968.

Proteomic databases

PRIDEiQ0P5F0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000027968; ENSBTAP00000027968; ENSBTAG00000021002.
GeneIDi535407.
KEGGibta:535407.

Organism-specific databases

CTDi53.

Phylogenomic databases

eggNOGiNOG85977.
GeneTreeiENSGT00530000062956.
HOGENOMiHOG000231439.
HOVERGENiHBG002203.
InParanoidiQ0P5F0.
KOiK14410.
OMAiQDFLHLT.
OrthoDBiEOG7GXPBJ.
TreeFamiTF312893.

Miscellaneous databases

NextBioi20876735.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal pons.

Entry informationi

Entry nameiPPAL_BOVIN
AccessioniPrimary (citable) accession number: Q0P5F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: September 19, 2006
Last modified: January 7, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.