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Protein

Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

ALG8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8 homolog
Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:ALG8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST
Transmembranei103 – 12220HelicalSequence analysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence analysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence analysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Transmembranei334 – 35421HelicalSequence analysisAdd
BLAST
Transmembranei368 – 38821HelicalSequence analysisAdd
BLAST
Transmembranei389 – 40921HelicalSequence analysisAdd
BLAST
Transmembranei427 – 44923HelicalSequence analysisAdd
BLAST
Transmembranei461 – 48121HelicalSequence analysisAdd
BLAST
Transmembranei488 – 50821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferasePRO_0000281997Add
BLAST

Proteomic databases

PaxDbiQ0P5D9.
PRIDEiQ0P5D9.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000049035.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2576. Eukaryota.
ENOG410XPPM. LUCA.
HOGENOMiHOG000168174.
HOVERGENiHBG024332.
InParanoidiQ0P5D9.
KOiK03849.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0P5D9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFGIATGG SNWFSALALG VTLLKCLLIP TYHSTDFEVH RNWLAITHSL
60 70 80 90 100
PISQWYYEAT SEWTLDYPPF FAWFEYALSH VAKYFDQEML NVRNLNYSSS
110 120 130 140 150
RTLLFQRFSV IFTDALFVYA VHECCKCIDG KKAGKELTEK PKFILSALLL
160 170 180 190 200
WNFGLLIVDH IHFQYNGFLS GLMLLSIARF FQKRHMEGAF LFAVLLHFKH
210 220 230 240 250
IYLYVAPAYG VYLLRSYCFT ANKQDGSIRW NSFSFVRLIS LGLIVFLVSA
260 270 280 290 300
LSLGPFLALN QLPQVFSRLF PFKRGLCHAY WAPNFWALYS AFDKVLSVIG
310 320 330 340 350
LELKLLDPNK IPKASMTSGL VQQFQHTVLP SVTPLATFIC TLIAMLPSVF
360 370 380 390 400
CLWCKPQGPR GFLRCLILCA LSSFMFGWHV HEKAILLAVL PMSLLSVGKA
410 420 430 440 450
VDASIFLILT TTGHYSPFPL LFTAPELPIK IILMLLFTIY SISSLKTLFR
460 470 480 490 500
KEKPLFNWME TFYLLGLGPL EVFCEFVFPF TSWNLKYPFI PLLLTSVYCA
510 520
VGITYAWLKL YVSVLIDPPV GKTKKQ
Length:526
Mass (Da):59,964
Last modified:September 19, 2006 - v1
Checksum:i75C49BD44E1A5F92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120179 mRNA. Translation: AAI20180.1.
RefSeqiNP_001069593.1. NM_001076125.2.
UniGeneiBt.104280.

Genome annotation databases

GeneIDi538731.
KEGGibta:538731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120179 mRNA. Translation: AAI20180.1.
RefSeqiNP_001069593.1. NM_001076125.2.
UniGeneiBt.104280.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000049035.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Proteomic databases

PaxDbiQ0P5D9.
PRIDEiQ0P5D9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi538731.
KEGGibta:538731.

Organism-specific databases

CTDi79053.

Phylogenomic databases

eggNOGiKOG2576. Eukaryota.
ENOG410XPPM. LUCA.
HOGENOMiHOG000168174.
HOVERGENiHBG024332.
InParanoidiQ0P5D9.
KOiK03849.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.

Entry informationi

Entry nameiALG8_BOVIN
AccessioniPrimary (citable) accession number: Q0P5D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: September 19, 2006
Last modified: July 6, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.