Reviewed, UniProtKB/Swiss-Prot Q0P5D9 (ALG8_BOVIN)

Names and origin

Protein names

Recommended name:
    Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
    Asparagine-linked glycosylation protein 8

Gene names

Name:

ALG8

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG6/ALG8 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,3-mannosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase		PRO_0000281997
Regions
	Transmembrane	4 – 24	21	Potential
	Transmembrane	103 – 122	20	Potential
	Transmembrane	143 – 163	21	Potential
	Transmembrane	188 – 208	21	Potential
	Transmembrane	238 – 258	21	Potential
	Transmembrane	334 – 354	21	Potential
	Transmembrane	368 – 388	21	Potential
	Transmembrane	389 – 409	21	Potential
	Transmembrane	427 – 449	23	Potential
	Transmembrane	461 – 481	21	Potential
	Transmembrane	488 – 508	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0P5D9-1 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: 75C49BD44E1A5F92	FASTA	526	59,964
	10 20 30 40 50 60 MAAFGIATGG SNWFSALALG VTLLKCLLIP TYHSTDFEVH RNWLAITHSL PISQWYYEAT 70 80 90 100 110 120 SEWTLDYPPF FAWFEYALSH VAKYFDQEML NVRNLNYSSS RTLLFQRFSV IFTDALFVYA 130 140 150 160 170 180 VHECCKCIDG KKAGKELTEK PKFILSALLL WNFGLLIVDH IHFQYNGFLS GLMLLSIARF 190 200 210 220 230 240 FQKRHMEGAF LFAVLLHFKH IYLYVAPAYG VYLLRSYCFT ANKQDGSIRW NSFSFVRLIS 250 260 270 280 290 300 LGLIVFLVSA LSLGPFLALN QLPQVFSRLF PFKRGLCHAY WAPNFWALYS AFDKVLSVIG 310 320 330 340 350 360 LELKLLDPNK IPKASMTSGL VQQFQHTVLP SVTPLATFIC TLIAMLPSVF CLWCKPQGPR 370 380 390 400 410 420 GFLRCLILCA LSSFMFGWHV HEKAILLAVL PMSLLSVGKA VDASIFLILT TTGHYSPFPL 430 440 450 460 470 480 LFTAPELPIK IILMLLFTIY SISSLKTLFR KEKPLFNWME TFYLLGLGPL EVFCEFVFPF 490 500 510 520 TSWNLKYPFI PLLLTSVYCA VGITYAWLKL YVSVLIDPPV GKTKKQ

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC120179 mRNA. Translation: AAI20180.1.
IPI	IPI00687036.
RefSeq	NP_001069593.1.
UniGene	Bt.18863
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0P5D9.
Protein family/group databases
CAZy	GT57. Glycosyltransferase Family 57.
Genome annotation databases
Ensembl	ENSBTAT00000057333; ENSBTAP00000049035; ENSBTAG00000018192; Bos taurus. [Genome view]
GeneID	538731.
KEGG	bta:538731.
Organism-specific databases
CTD	538731.
Phylogenomic databases
HOVERGEN	Q0P5D9.
Family and domain databases
InterPro	IPR004856. Glycosyltransferase_ALG6/ALG8. [Graphical view]
PANTHER	PTHR12413. Alg6_Alg8. 1 hit.
Pfam	PF03155. Alg6_Alg8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG8_BOVIN

Accession

Primary (citable) accession number: Q0P5D9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	September 19, 2006
Last modified:	October 13, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)