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Protein

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Gene

MTHFD2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.By similarity

Miscellaneous

This NAD-dependent bifunctional enzyme has very different kinetic properties than the larger NADP-dependent trifunctional enzyme and is unique in that it requires formation of an enzyme-magnesium complex to allow binding of NAD.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei233NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi200 – 202NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Oxidoreductase
Biological processOne-carbon metabolism
LigandMagnesium, NAD, NADP

Enzyme and pathway databases

ReactomeiR-BTA-196757. Metabolism of folate and pterines.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Including the following 2 domains:
NAD-dependent methylenetetrahydrofolate dehydrogenase (EC:1.5.1.15)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Gene namesi
Name:MTHFD2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35MitochondrionBy similarityAdd BLAST35
ChainiPRO_000030590236 – 350Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrialAdd BLAST315

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50N6-acetyllysine; alternateBy similarity1
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ0P5C2.
PRIDEiQ0P5C2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000004881.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006419.

Structurei

3D structure databases

ProteinModelPortaliQ0P5C2.
SMRiQ0P5C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 88Substrate bindingBy similarity5
Regioni131 – 133Substrate bindingBy similarity3
Regioni309 – 313Substrate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0089. Eukaryota.
COG0190. LUCA.
GeneTreeiENSGT00880000137956.
HOGENOMiHOG000218242.
HOVERGENiHBG006411.
InParanoidiQ0P5C2.
KOiK13403.
OMAiAGKLCGD.
OrthoDBiEOG091G0VB7.
TreeFamiTF323998.

Family and domain databases

HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
PANTHERiPTHR10025. PTHR10025. 1 hit.
PfamiView protein in Pfam
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0P5C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAASFITSL VTRLLRSAQS GRLHQRPFHL SAVRNEAVVI SGRKLAEQIK
60 70 80 90 100
QEVRQEVEEW VASGNKRPHL SVVLVGENPA SQSYVLNKTR AAASVGINSE
110 120 130 140 150
TILKPASISE EELLNLINKL NNDDNVDGLL VQLPLPEHID ERKVCNAVSP
160 170 180 190 200
DKDVDGFHVI NVGRMCLDQC SMLPATPWGV WEIIKRTGIP TLGKNVVVAG
210 220 230 240 250
RSKNVGMPIA MLLHTDGAHE RPGGDATVTI SHRYTPKEEL KKHTALADIV
260 270 280 290 300
ISAAGIPNLI TADMIKEGAA VIDVGINRIQ DPITAKPKLV GDVDFEGVKK
310 320 330 340 350
KAGYITPVPG GVGPMTVAML MKNTIIAAKK VLRLEEQEVL KSKELGVASN
Length:350
Mass (Da):37,767
Last modified:September 19, 2006 - v1
Checksum:iD71A67DFE1FEE05F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120249 mRNA. Translation: AAI20250.1.
RefSeqiNP_001069223.1. NM_001075755.2.
UniGeneiBt.6685.

Genome annotation databases

EnsembliENSBTAT00000006419; ENSBTAP00000006419; ENSBTAG00000004881.
GeneIDi517539.
KEGGibta:517539.

Similar proteinsi

Entry informationi

Entry nameiMTDC_BOVIN
AccessioniPrimary (citable) accession number: Q0P5C2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: September 19, 2006
Last modified: August 30, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families