Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q0P5C2 (MTDC_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Including the following 2 domains:

  1. NAD-dependent methylenetetrahydrofolate dehydrogenase
    EC=1.5.1.15
  2. Methenyltetrahydrofolate cyclohydrolase
    EC=3.5.4.9
Gene names
Name:MTHFD2
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH.

5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.

Cofactor

Magnesium By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion By similarity.

Miscellaneous

This NAD-dependent bifunctional enzyme has very different kinetic properties than the larger NADP-dependent trifunctional enzyme and is unique in that it requires formation of an enzyme-magnesium complex to allow binding of NAD By similarity.

Sequence similarities

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3535Mitochondrion By similarity
Chain36 – 350315Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
PRO_0000305902

Amino acid modifications

Modified residue501N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0P5C2 [UniParc].

Last modified September 19, 2006. Version 1.
Checksum: D71A67DFE1FEE05F

FASTA35037,767
        10         20         30         40         50         60 
MAAASFITSL VTRLLRSAQS GRLHQRPFHL SAVRNEAVVI SGRKLAEQIK QEVRQEVEEW 

        70         80         90        100        110        120 
VASGNKRPHL SVVLVGENPA SQSYVLNKTR AAASVGINSE TILKPASISE EELLNLINKL 

       130        140        150        160        170        180 
NNDDNVDGLL VQLPLPEHID ERKVCNAVSP DKDVDGFHVI NVGRMCLDQC SMLPATPWGV 

       190        200        210        220        230        240 
WEIIKRTGIP TLGKNVVVAG RSKNVGMPIA MLLHTDGAHE RPGGDATVTI SHRYTPKEEL 

       250        260        270        280        290        300 
KKHTALADIV ISAAGIPNLI TADMIKEGAA VIDVGINRIQ DPITAKPKLV GDVDFEGVKK 

       310        320        330        340        350 
KAGYITPVPG GVGPMTVAML MKNTIIAAKK VLRLEEQEVL KSKELGVASN

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Placenta.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC120249 mRNA. Translation: AAI20250.1.
IPIIPI00712161.
RefSeqNP_001069223.1. NM_001075755.1.
UniGeneBt.6685.

3D structure databases

ProteinModelPortalQ0P5C2.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0P5C2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000006419; ENSBTAP00000006419; ENSBTAG00000004881.
GeneID517539.
KEGGbta:517539.

Organism-specific databases

CTD10797.

Phylogenomic databases

eggNOGmaNOG11296.
GeneTreeENSGT00600000084403.
HOVERGENHBG006411.
InParanoidQ0P5C2.
OMANQDERVD.
OrthoDBEOG4N5VZ4.
PhylomeDBQ0P5C2.

Family and domain databases

InterProIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK13403.
PfamPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSPR00085. THFDHDRGNASE.
PROSITEPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTDC_BOVIN
AccessionPrimary (citable) accession number: Q0P5C2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: September 19, 2006
Last modified: December 14, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents