Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Gene

MTHFD2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

Magnesium, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Including the following 2 domains:
NAD-dependent methylenetetrahydrofolate dehydrogenase (EC:1.5.1.15)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Gene namesi
Name:MTHFD2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535MitochondrionBy similarityAdd
BLAST
Chaini36 – 350315Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrialPRO_0000305902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ0P5C2.
PRIDEiQ0P5C2.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006419.

Structurei

3D structure databases

ProteinModelPortaliQ0P5C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0089. Eukaryota.
COG0190. LUCA.
GeneTreeiENSGT00840000129764.
HOGENOMiHOG000218242.
HOVERGENiHBG006411.
InParanoidiQ0P5C2.
KOiK13403.
OMAiCLDQSTM.
OrthoDBiEOG7CG70C.
TreeFamiTF323998.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0P5C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAASFITSL VTRLLRSAQS GRLHQRPFHL SAVRNEAVVI SGRKLAEQIK
60 70 80 90 100
QEVRQEVEEW VASGNKRPHL SVVLVGENPA SQSYVLNKTR AAASVGINSE
110 120 130 140 150
TILKPASISE EELLNLINKL NNDDNVDGLL VQLPLPEHID ERKVCNAVSP
160 170 180 190 200
DKDVDGFHVI NVGRMCLDQC SMLPATPWGV WEIIKRTGIP TLGKNVVVAG
210 220 230 240 250
RSKNVGMPIA MLLHTDGAHE RPGGDATVTI SHRYTPKEEL KKHTALADIV
260 270 280 290 300
ISAAGIPNLI TADMIKEGAA VIDVGINRIQ DPITAKPKLV GDVDFEGVKK
310 320 330 340 350
KAGYITPVPG GVGPMTVAML MKNTIIAAKK VLRLEEQEVL KSKELGVASN
Length:350
Mass (Da):37,767
Last modified:September 19, 2006 - v1
Checksum:iD71A67DFE1FEE05F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120249 mRNA. Translation: AAI20250.1.
RefSeqiNP_001069223.1. NM_001075755.2.
UniGeneiBt.6685.

Genome annotation databases

EnsembliENSBTAT00000006419; ENSBTAP00000006419; ENSBTAG00000004881.
GeneIDi517539.
KEGGibta:517539.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120249 mRNA. Translation: AAI20250.1.
RefSeqiNP_001069223.1. NM_001075755.2.
UniGeneiBt.6685.

3D structure databases

ProteinModelPortaliQ0P5C2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006419.

Proteomic databases

PaxDbiQ0P5C2.
PRIDEiQ0P5C2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006419; ENSBTAP00000006419; ENSBTAG00000004881.
GeneIDi517539.
KEGGibta:517539.

Organism-specific databases

CTDi10797.

Phylogenomic databases

eggNOGiKOG0089. Eukaryota.
COG0190. LUCA.
GeneTreeiENSGT00840000129764.
HOGENOMiHOG000218242.
HOVERGENiHBG006411.
InParanoidiQ0P5C2.
KOiK13403.
OMAiCLDQSTM.
OrthoDBiEOG7CG70C.
TreeFamiTF323998.

Miscellaneous databases

NextBioi20872464.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Placenta.

Entry informationi

Entry nameiMTDC_BOVIN
AccessioniPrimary (citable) accession number: Q0P5C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: September 19, 2006
Last modified: May 11, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This NAD-dependent bifunctional enzyme has very different kinetic properties than the larger NADP-dependent trifunctional enzyme and is unique in that it requires formation of an enzyme-magnesium complex to allow binding of NAD.By similarity

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.