Reviewed, UniProtKB/Swiss-Prot Q0P5C2 (MTDC_BOVIN)

Names and origin

Protein names

Recommended name:
    Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Including the following 2 domains:
    1- Recommended name:
            NAD-dependent methylenetetrahydrofolate dehydrogenase
              EC=1.5.1.15
    2- Recommended name:
            Methenyltetrahydrofolate cyclohydrolase
              EC=3.5.4.9

Gene names

Name:

MTHFD2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion By similarity.
Miscellaneous	This NAD-dependent bifunctional enzyme has very different kinetic properties than the larger NADP-dependent trifunctional enzyme and is unique in that it requires formation of an enzyme-magnesium complex to allow binding of NAD By similarity.
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium NAD
Molecular function	Hydrolase Oxidoreductase
PTM	Acetylation
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: EC methylenetetrahydrofolate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion By similarity
	Chain	36 – 350	315	Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial		PRO_0000305902
Amino acid modifications
	Modified residue	50	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0P5C2-1 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: D71A67DFE1FEE05F	FASTA	350	37,767
	10 20 30 40 50 60 MAAASFITSL VTRLLRSAQS GRLHQRPFHL SAVRNEAVVI SGRKLAEQIK QEVRQEVEEW 70 80 90 100 110 120 VASGNKRPHL SVVLVGENPA SQSYVLNKTR AAASVGINSE TILKPASISE EELLNLINKL 130 140 150 160 170 180 NNDDNVDGLL VQLPLPEHID ERKVCNAVSP DKDVDGFHVI NVGRMCLDQC SMLPATPWGV 190 200 210 220 230 240 WEIIKRTGIP TLGKNVVVAG RSKNVGMPIA MLLHTDGAHE RPGGDATVTI SHRYTPKEEL 250 260 270 280 290 300 KKHTALADIV ISAAGIPNLI TADMIKEGAA VIDVGINRIQ DPITAKPKLV GDVDFEGVKK 310 320 330 340 350 KAGYITPVPG GVGPMTVAML MKNTIIAAKK VLRLEEQEVL KSKELGVASN

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Placenta.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC120249 mRNA. Translation: AAI20250.1.
IPI	IPI00712161.
RefSeq	NP_001069223.1.
UniGene	Bt.6685
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0P5C2.
Genome annotation databases
Ensembl	ENSBTAT00000006419; ENSBTAP00000006419; ENSBTAG00000004881; Bos taurus. [Genome view]
GeneID	517539.
KEGG	bta:517539.
Organism-specific databases
CTD	517539.
Phylogenomic databases
HOVERGEN	Q0P5C2.
OMA	HSKTRDL.
Enzyme and pathway databases
BRENDA	1.5.1.15. 251. 3.5.4.9. 251.
Family and domain databases
InterPro	IPR000672. THF_DH/CycHdrlase. [Graphical view]
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
ProDom	PD002300. THFDhg/Cyc_hydro. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00766. THF_DHG_CYH_1. 1 hit. PS00767. THF_DHG_CYH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTDC_BOVIN

Accession

Primary (citable) accession number: Q0P5C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	September 19, 2006
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)