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Q0P594

- PP2AB_BOVIN

UniProt

Q0P594 - PP2AB_BOVIN

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Protein

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

Gene

PPP2CB

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Manganese 1By similarity
Metal bindingi59 – 591Manganese 1By similarity
Metal bindingi85 – 851Manganese 1By similarity
Metal bindingi85 – 851Manganese 2By similarity
Metal bindingi117 – 1171Manganese 2By similarity
Active sitei118 – 1181Proton donorBy similarity
Metal bindingi167 – 1671Manganese 2By similarity
Metal bindingi241 – 2411Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. apoptotic mitochondrial changes Source: Ensembl
  2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
  3. regulation of gene expression Source: Ensembl
  4. response to antibiotic Source: Ensembl
  5. response to endoplasmic reticulum stress Source: Ensembl
  6. response to hydrogen peroxide Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_202853. Spry regulation of FGF signaling.
REACT_203739. Degradation of beta-catenin by the destruction complex.
REACT_205490. DARPP-32 events.
REACT_209545. Resolution of Sister Chromatid Cohesion.
REACT_209873. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_212572. PP2A-mediated dephosphorylation of key metabolic factors.
REACT_212887. Separation of Sister Chromatids.
REACT_213811. CTLA4 inhibitory signaling.
REACT_217101. Integration of energy metabolism.
REACT_217581. Beta-catenin phosphorylation cascade.
REACT_218138. Cyclin D associated events in G1.
REACT_220398. ERKs are inactivated.
REACT_224007. Cyclin A/B1 associated events during G2/M transition.
REACT_224187. Glycolysis.
REACT_224304. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
REACT_226850. ERK/MAPK targets.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (EC:3.1.3.16)
Short name:
PP2A-beta
Gene namesi
Name:PPP2CB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 27

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Chromosomecentromere By similarity. Cytoplasmcytoskeletonspindle pole By similarity
Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles (By similarity).By similarity

GO - Cellular componenti

  1. chromosome, centromeric region Source: UniProtKB-KW
  2. cytoplasm Source: UniProtKB
  3. cytoskeleton Source: UniProtKB-KW
  4. extracellular vesicular exosome Source: Ensembl
  5. nucleus Source: UniProtKB-KW
  6. protein phosphatase type 2A complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Serine/threonine-protein phosphatase 2A catalytic subunit beta isoformPRO_0000283053Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei307 – 3071PhosphotyrosineBy similarity
Modified residuei309 – 3091Leucine methyl esterBy similarity

Post-translational modificationi

Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization (By similarity).By similarity
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation (By similarity).By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ0P594.

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1 (By similarity). May indirectly interact with SGOL1, most probably through regulatory B56 subunits (By similarity). Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with TBCD. Interacts with CTTNBP2NL (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012182.

Structurei

3D structure databases

ProteinModelPortaliQ0P594.
SMRiQ0P594. Positions 2-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00550000074618.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiQ0P594.
KOiK04382.
OMAiEHIRALD.
OrthoDBiEOG74N5H2.
TreeFamiTF105559.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0P594-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDKAFTKDL DQWVEQLNEC KQLNENQVRT LCEKAKEILT KESNVQEVRC
60 70 80 90 100
PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL
110 120 130 140 150
VALKVRYPER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF
160 170 180 190 200
DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW
210 220 230 240 250
SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW
260 270 280 290 300
CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV

TRRTPDYFL
Length:309
Mass (Da):35,561
Last modified:September 19, 2006 - v1
Checksum:i50AD45DD126F8485
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120332 mRNA. Translation: AAI20333.1.
RefSeqiNP_001069325.1. NM_001075857.1.
UniGeneiBt.53812.

Genome annotation databases

EnsembliENSBTAT00000012182; ENSBTAP00000012182; ENSBTAG00000009245.
GeneIDi524361.
KEGGibta:524361.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120332 mRNA. Translation: AAI20333.1 .
RefSeqi NP_001069325.1. NM_001075857.1.
UniGenei Bt.53812.

3D structure databases

ProteinModelPortali Q0P594.
SMRi Q0P594. Positions 2-309.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000012182.

Proteomic databases

PaxDbi Q0P594.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000012182 ; ENSBTAP00000012182 ; ENSBTAG00000009245 .
GeneIDi 524361.
KEGGi bta:524361.

Organism-specific databases

CTDi 5516.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00550000074618.
HOGENOMi HOG000172696.
HOVERGENi HBG000216.
InParanoidi Q0P594.
KOi K04382.
OMAi EHIRALD.
OrthoDBi EOG74N5H2.
TreeFami TF105559.

Enzyme and pathway databases

Reactomei REACT_202853. Spry regulation of FGF signaling.
REACT_203739. Degradation of beta-catenin by the destruction complex.
REACT_205490. DARPP-32 events.
REACT_209545. Resolution of Sister Chromatid Cohesion.
REACT_209873. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_212572. PP2A-mediated dephosphorylation of key metabolic factors.
REACT_212887. Separation of Sister Chromatids.
REACT_213811. CTLA4 inhibitory signaling.
REACT_217101. Integration of energy metabolism.
REACT_217581. Beta-catenin phosphorylation cascade.
REACT_218138. Cyclin D associated events in G1.
REACT_220398. ERKs are inactivated.
REACT_224007. Cyclin A/B1 associated events during G2/M transition.
REACT_224187. Glycolysis.
REACT_224304. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
REACT_226850. ERK/MAPK targets.

Miscellaneous databases

NextBioi 20873952.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal muscle.
  2. "Cytosolic Arl2 is complexed with cofactor D and protein phosphatase 2A."
    Shern J.F., Sharer J.D., Pallas D.C., Bartolini F., Cowan N.J., Reed M.S., Pohl J., Kahn R.A.
    J. Biol. Chem. 278:40829-40836(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TBCD, IDENTIFICATION IN A COMPLEX WITH ARL2; PPP2R1A; PPP2R2A; PPP2R5E AND TBCD, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPP2AB_BOVIN
AccessioniPrimary (citable) accession number: Q0P594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: September 19, 2006
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3