Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q0P594

- PP2AB_BOVIN

UniProt

Q0P594 - PP2AB_BOVIN

Protein

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

Gene

PPP2CB

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (19 Sep 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Manganese 1By similarity
    Metal bindingi59 – 591Manganese 1By similarity
    Metal bindingi85 – 851Manganese 1By similarity
    Metal bindingi85 – 851Manganese 2By similarity
    Metal bindingi117 – 1171Manganese 2By similarity
    Active sitei118 – 1181Proton donorBy similarity
    Metal bindingi167 – 1671Manganese 2By similarity
    Metal bindingi241 – 2411Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. apoptotic mitochondrial changes Source: Ensembl
    2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
    3. regulation of gene expression Source: Ensembl
    4. response to antibiotic Source: Ensembl
    5. response to endoplasmic reticulum stress Source: Ensembl
    6. response to hydrogen peroxide Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_202853. Spry regulation of FGF signaling.
    REACT_203739. Degradation of beta-catenin by the destruction complex.
    REACT_205490. DARPP-32 events.
    REACT_209545. Resolution of Sister Chromatid Cohesion.
    REACT_209873. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_212572. PP2A-mediated dephosphorylation of key metabolic factors.
    REACT_212887. Separation of Sister Chromatids.
    REACT_213811. CTLA4 inhibitory signaling.
    REACT_217101. Integration of energy metabolism.
    REACT_217581. Beta-catenin phosphorylation cascade.
    REACT_218138. Cyclin D associated events in G1.
    REACT_220398. ERKs are inactivated.
    REACT_224007. Cyclin A/B1 associated events during G2/M transition.
    REACT_224187. Glycolysis.
    REACT_224304. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
    REACT_226850. ERK/MAPK targets.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (EC:3.1.3.16)
    Short name:
    PP2A-beta
    Gene namesi
    Name:PPP2CB
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 27

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity. Chromosomecentromere By similarity. Cytoplasmcytoskeletonspindle pole By similarity
    Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles By similarity.By similarity

    GO - Cellular componenti

    1. chromosome, centromeric region Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB
    3. nucleus Source: UniProtKB-SubCell
    4. protein phosphatase type 2A complex Source: Ensembl
    5. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Serine/threonine-protein phosphatase 2A catalytic subunit beta isoformPRO_0000283053Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei307 – 3071PhosphotyrosineBy similarity
    Modified residuei309 – 3091Leucine methyl esterBy similarity

    Post-translational modificationi

    Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.By similarity
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation By similarity.By similarity

    Keywords - PTMi

    Methylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ0P594.

    Interactioni

    Subunit structurei

    PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1 By similarity. May indirectly interact with SGOL1, most probably through regulatory B56 subunits By similarity. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with TBCD. Interacts with CTTNBP2NL By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000012182.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0P594.
    SMRiQ0P594. Positions 2-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00550000074618.
    HOGENOMiHOG000172696.
    HOVERGENiHBG000216.
    InParanoidiQ0P594.
    KOiK04382.
    OMAiEHIRALD.
    OrthoDBiEOG74N5H2.
    TreeFamiTF105559.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0P594-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDKAFTKDL DQWVEQLNEC KQLNENQVRT LCEKAKEILT KESNVQEVRC    50
    PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL 100
    VALKVRYPER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF 150
    DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW 200
    SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW 250
    CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV 300
    TRRTPDYFL 309
    Length:309
    Mass (Da):35,561
    Last modified:September 19, 2006 - v1
    Checksum:i50AD45DD126F8485
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120332 mRNA. Translation: AAI20333.1.
    RefSeqiNP_001069325.1. NM_001075857.1.
    UniGeneiBt.53812.

    Genome annotation databases

    EnsembliENSBTAT00000012182; ENSBTAP00000012182; ENSBTAG00000009245.
    GeneIDi524361.
    KEGGibta:524361.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120332 mRNA. Translation: AAI20333.1 .
    RefSeqi NP_001069325.1. NM_001075857.1.
    UniGenei Bt.53812.

    3D structure databases

    ProteinModelPortali Q0P594.
    SMRi Q0P594. Positions 2-309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000012182.

    Proteomic databases

    PaxDbi Q0P594.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000012182 ; ENSBTAP00000012182 ; ENSBTAG00000009245 .
    GeneIDi 524361.
    KEGGi bta:524361.

    Organism-specific databases

    CTDi 5516.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00550000074618.
    HOGENOMi HOG000172696.
    HOVERGENi HBG000216.
    InParanoidi Q0P594.
    KOi K04382.
    OMAi EHIRALD.
    OrthoDBi EOG74N5H2.
    TreeFami TF105559.

    Enzyme and pathway databases

    Reactomei REACT_202853. Spry regulation of FGF signaling.
    REACT_203739. Degradation of beta-catenin by the destruction complex.
    REACT_205490. DARPP-32 events.
    REACT_209545. Resolution of Sister Chromatid Cohesion.
    REACT_209873. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_212572. PP2A-mediated dephosphorylation of key metabolic factors.
    REACT_212887. Separation of Sister Chromatids.
    REACT_213811. CTLA4 inhibitory signaling.
    REACT_217101. Integration of energy metabolism.
    REACT_217581. Beta-catenin phosphorylation cascade.
    REACT_218138. Cyclin D associated events in G1.
    REACT_220398. ERKs are inactivated.
    REACT_224007. Cyclin A/B1 associated events during G2/M transition.
    REACT_224187. Glycolysis.
    REACT_224304. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
    REACT_226850. ERK/MAPK targets.

    Miscellaneous databases

    NextBioi 20873952.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal muscle.
    2. "Cytosolic Arl2 is complexed with cofactor D and protein phosphatase 2A."
      Shern J.F., Sharer J.D., Pallas D.C., Bartolini F., Cowan N.J., Reed M.S., Pohl J., Kahn R.A.
      J. Biol. Chem. 278:40829-40836(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TBCD, IDENTIFICATION IN A COMPLEX WITH ARL2; PPP2R1A; PPP2R2A; PPP2R5E AND TBCD, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPP2AB_BOVIN
    AccessioniPrimary (citable) accession number: Q0P594
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: September 19, 2006
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3