Q0P543 (GIPR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastric inhibitory polypeptide receptor
Short name=GIP-R

Alternative name(s):
Glucose-dependent insulinotropic polypeptide receptor

Gene names

Name:

Gipr

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase By similarity.
Subunit structure	May form homodimers and heterodimers with GLP1R By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	N-glycosylation is required for cell surface expression and lengthens receptor half-life by preventing degradation in the ER By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	desensitization of G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara endocrine pancreas development Inferred from mutant phenotype PubMed 21270265. Source: MGI neuropeptide signaling pathway Traceable author statement PubMed 10611300. Source: MGI positive regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of insulin secretion Inferred from electronic annotation. Source: Compara response to axon injury Inferred from electronic annotation. Source: Compara response to calcium ion Inferred from electronic annotation. Source: Compara response to fatty acid Inferred from electronic annotation. Source: Compara response to glucose stimulus Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	gastric inhibitory peptide receptor activity Inferred from electronic annotation. Source: Compara peptide hormone binding Inferred from electronic annotation. Source: Compara receptor activity Traceable author statement PubMed 10611300. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q0P543-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q0P543-2)

The sequence of this isoform differs from the canonical sequence as follows:
     208-230: ALAACRTAQIMTQYCVGANYTWL → VLHRLLPGGTKTFPIYFRTFPHH
     231-460: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 460	442	Gastric inhibitory polypeptide receptor		PRO_0000306251
Regions
	Topological domain	19 – 134	116	Extracellular Potential
	Transmembrane	135 – 155	21	Helical; Name=1; Potential
	Topological domain	156 – 222	67	Cytoplasmic Potential
	Transmembrane	223 – 243	21	Helical; Name=2; Potential
	Topological domain	244 – 255	12	Extracellular Potential
	Transmembrane	256 – 276	21	Helical; Name=3; Potential
	Topological domain	277 – 297	21	Cytoplasmic Potential
	Transmembrane	298 – 318	21	Helical; Name=4; Potential
	Topological domain	319 – 337	19	Extracellular Potential
	Transmembrane	338 – 358	21	Helical; Name=5; Potential
	Topological domain	359 – 370	12	Cytoplasmic Potential
	Transmembrane	371 – 391	21	Helical; Name=6; Potential
	Topological domain	392 – 460	69	Extracellular Potential
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	73	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 66		By similarity
	Disulfide bond	57 ↔ 99		By similarity
	Disulfide bond	80 ↔ 114		By similarity
Natural variations
	Alternative sequence	208 – 230	23	ALAAC…NYTWL → VLHRLLPGGTKTFPIYFRTF PHH in isoform 2.		VSP_028433
	Alternative sequence	231 – 460	230	Missing in isoform 2.		VSP_028434
Experimental info
	Sequence conflict	122	1	A → V in AAO85080. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 2, 2007. Version 2. Checksum: 41ABC3F1E0A7DF98	FASTA	460	52,992
	10 20 30 40 50 60 MPLRLLLLLL WLWGLQWAET DSEGQTTTGE LYQRWEHYGQ ECQKMLETTE PPSGLACNGS 70 80 90 100 110 120 FDMYACWNYT AANTTARVSC PWYLPWFRQV SAGFVFRQCG SDGQWGSWRD HTQCENPEKN 130 140 150 160 170 180 GAFQDQTLIL ERLQIMYTVG YSLSLTTLLL ALLILSLFRR LHCTRNYIHM NLFTSFMLRA 190 200 210 220 230 240 AAILTRDQLL PPLGPYTGDQ APTPWNQALA ACRTAQIMTQ YCVGANYTWL LVEGVYLHHL 250 260 270 280 290 300 LVIVGRSEKG HFRCYLLLGW GAPALFVIPW VIVRYLRENT QCWERNEVKA IWWIIRTPIL 310 320 330 340 350 360 ITILINFLIF IRILGILVSK LRTRQMRCPD YRLRLARSTL TLVPLLGVHE VVFAPVTEEQ 370 380 390 400 410 420 VEGSLRFAKL AFEIFLSSFQ GFLVSVLYCF INKEVQSEIR QGWRHRRLRL SLQEQRPRPH 430 440 450 460 QELAPRAVPL SSACREAAVG NALPSGMLHV PGDEVLESYC
	Isoform 2 [UniParc]. Checksum: AA4800B55C77468E	FASTA	230	26,617
	10 20 30 40 50 60 MPLRLLLLLL WLWGLQWAET DSEGQTTTGE LYQRWEHYGQ ECQKMLETTE PPSGLACNGS 70 80 90 100 110 120 FDMYACWNYT AANTTARVSC PWYLPWFRQV SAGFVFRQCG SDGQWGSWRD HTQCENPEKN 130 140 150 160 170 180 GAFQDQTLIL ERLQIMYTVG YSLSLTTLLL ALLILSLFRR LHCTRNYIHM NLFTSFMLRA 190 200 210 220 230 AAILTRDQLL PPLGPYTGDQ APTPWNQVLH RLLPGGTKTF PIYFRTFPHH

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"The G protein-coupled receptor repertoires of human and mouse." Vassilatis D.K., Hohmann J.G., Zeng H., Li F., Ranchalis J.E., Mortrud M.T., Brown A., Rodriguez S.S., Weller J.R., Wright A.C., Bergmann J.E., Gaitanaris G.A. Proc. Natl. Acad. Sci. U.S.A. 100:4903-4908(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-178.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC145199 Genomic DNA. No translation available. BC120671 mRNA. Translation: AAI20672.1. BC120673 mRNA. Translation: AAI20674.1. AY255568 mRNA. Translation: AAO85080.1.
IPI	IPI00659955. IPI00672590.
RefSeq	NP_001074284.1. NM_001080815.1.
UniGene	Mm.333633.
3D structure databases
ProteinModelPortal	Q0P543.
SMR	Q0P543. Positions 27-118.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000092384.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q0P543.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000094790; ENSMUSP00000092384; ENSMUSG00000030406.
GeneID	381853.
KEGG	mmu:381853.
UCSC	uc009fkw.1. mouse. uc009fkx.1. mouse.
Organism-specific databases
CTD	2696.
MGI	MGI:1352753. Gipr.
Phylogenomic databases
eggNOG	NOG239460.
GeneTree	ENSGT00580000081498.
HOGENOM	HOG000008250.
HOVERGEN	HBG008318.
InParanoid	Q0P543.
KO	K04580.
OMA	TRQMRCP.
OrthoDB	EOG469QTS.
Gene expression databases
Bgee	Q0P543.
CleanEx	MM_GIPR.
Genevestigator	Q0P543.
Family and domain databases
InterPro	IPR017981. GPCR_2-like. IPR001879. GPCR_2_extracellular_dom. IPR001749. GPCR_2_GIP_rcpt. IPR000832. GPCR_2_secretin-like. IPR017983. GPCR_2_secretin-like_CS. [Graphical view]
Pfam	PF00002. 7tm_2. 1 hit. PF02793. HRM. 1 hit. [Graphical view]
PRINTS	PR01129. GIPRECEPTOR. PR00249. GPCRSECRETIN.
SMART	SM00008. HormR. 1 hit. [Graphical view]
PROSITE	PS00649. G_PROTEIN_RECEP_F2_1. 1 hit. PS00650. G_PROTEIN_RECEP_F2_2. 1 hit. PS50227. G_PROTEIN_RECEP_F2_3. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	402687.
SOURCE	Search...

Entry information

Entry name

GIPR_MOUSE

Accession

Primary (citable) accession number: Q0P543
Secondary accession number(s): Q80UB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	April 3, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program