Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyprenol reductase

Gene

srd5a3

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) (By similarity).By similarity

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3 (EC:1.3.1.22)
Steroid 5-alpha-reductase 3
Short name:
S5AR 3
Short name:
SR type 3
Gene namesi
Name:srd5a3
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-985511. srd5a3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 2CytoplasmicSequence analysis2
Transmembranei3 – 23HelicalSequence analysisAdd BLAST21
Topological domaini24 – 65LumenalSequence analysisAdd BLAST42
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Topological domaini87 – 120CytoplasmicSequence analysisAdd BLAST34
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Topological domaini142 – 148LumenalSequence analysis7
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 184CytoplasmicSequence analysisAdd BLAST15
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Topological domaini206 – 255LumenalSequence analysisAdd BLAST50
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Topological domaini277 – 308CytoplasmicSequence analysisAdd BLAST32

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003177061 – 308Polyprenol reductaseAdd BLAST308

Proteomic databases

PaxDbiQ0P4J9.

Expressioni

Gene expression databases

BgeeiENSXETG00000021063.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000045522.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1640. Eukaryota.
ENOG4111JQM. LUCA.
HOVERGENiHBG057797.
InParanoidiQ0P4J9.
KOiK12345.
OrthoDBiEOG091G0F09.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0P4J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLALVWLL LDATFLITLL WHLLQGCKSG HSLLCSVFQD LIRYGKTKTG
60 70 80 90 100
LQRPAWLQWF DIPKRCFWHF YCVSLIWNGC LLWILLRLLL QSVPVPEWLQ
110 120 130 140 150
LVLHFLHAGS EPQILDRELS VILALALLWL HSLRRLLECL FVSVFSNGVI
160 170 180 190 200
HLVQYCFGLG YYFLIGITVL TYCPLDRRTV STDNLLTQCH WYHILGLALY
210 220 230 240 250
IWASLHQYRC HCILAGLRKS ASGNVINLNH SVPCGDWFER VSCPHYFAEL
260 270 280 290 300
LIYVSIAVVF GLLNTIWWLV VLYVLLNQAL AALLCHEFYH EKFDTYPIHR

KAFIPFIF
Length:308
Mass (Da):35,876
Last modified:September 19, 2006 - v1
Checksum:iFFE931DCDB0D558A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122039 mRNA. Translation: AAI22040.1.
RefSeqiNP_001072539.1. NM_001079071.1.
UniGeneiStr.24988.

Genome annotation databases

GeneIDi779994.
KEGGixtr:779994.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122039 mRNA. Translation: AAI22040.1.
RefSeqiNP_001072539.1. NM_001079071.1.
UniGeneiStr.24988.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000045522.

Proteomic databases

PaxDbiQ0P4J9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi779994.
KEGGixtr:779994.

Organism-specific databases

CTDi79644.
XenbaseiXB-GENE-985511. srd5a3.

Phylogenomic databases

eggNOGiKOG1640. Eukaryota.
ENOG4111JQM. LUCA.
HOVERGENiHBG057797.
InParanoidiQ0P4J9.
KOiK12345.
OrthoDBiEOG091G0F09.

Enzyme and pathway databases

UniPathwayiUPA00378.

Gene expression databases

BgeeiENSXETG00000021063.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPORED_XENTR
AccessioniPrimary (citable) accession number: Q0P4J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 19, 2006
Last modified: September 7, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.