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Q0P4J9

- PORED_XENTR

UniProt

Q0P4J9 - PORED_XENTR

Protein

Polyprenol reductase

Gene

srd5a3

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (19 Sep 2006)
      Previous versions | rss
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    Functioni

    Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) By similarity.By similarity

    Catalytic activityi

    Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
    A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

    Pathwayi

    GO - Molecular functioni

    1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: UniProtKB
    2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
    3. oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Source: UniProtKB

    GO - Biological processi

    1. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
    2. dolichol metabolic process Source: UniProtKB
    3. polyprenol catabolic process Source: UniProtKB
    4. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_210591. Androgen biosynthesis.
    REACT_211888. Synthesis of Dolichyl-phosphate.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyprenol reductase (EC:1.3.1.94)
    Alternative name(s):
    3-oxo-5-alpha-steroid 4-dehydrogenase 3 (EC:1.3.1.22)
    Steroid 5-alpha-reductase 3
    Short name:
    S5AR 3
    Short name:
    SR type 3
    Gene namesi
    Name:srd5a3
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-985511. srd5a3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 308308Polyprenol reductasePRO_0000317706Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiQ0P4J9.

    Interactioni

    Protein-protein interaction databases

    STRINGi8364.ENSXETP00000045522.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 22CytoplasmicSequence Analysis
    Topological domaini24 – 6542LumenalSequence AnalysisAdd
    BLAST
    Topological domaini87 – 12034CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini142 – 1487LumenalSequence Analysis
    Topological domaini170 – 18415CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini206 – 25550LumenalSequence AnalysisAdd
    BLAST
    Topological domaini277 – 30832CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei3 – 2321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei66 – 8621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei121 – 14121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei149 – 16921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei185 – 20521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei256 – 27621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG330066.
    HOVERGENiHBG057797.
    KOiK12345.

    Family and domain databases

    InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0P4J9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLLALVWLL LDATFLITLL WHLLQGCKSG HSLLCSVFQD LIRYGKTKTG    50
    LQRPAWLQWF DIPKRCFWHF YCVSLIWNGC LLWILLRLLL QSVPVPEWLQ 100
    LVLHFLHAGS EPQILDRELS VILALALLWL HSLRRLLECL FVSVFSNGVI 150
    HLVQYCFGLG YYFLIGITVL TYCPLDRRTV STDNLLTQCH WYHILGLALY 200
    IWASLHQYRC HCILAGLRKS ASGNVINLNH SVPCGDWFER VSCPHYFAEL 250
    LIYVSIAVVF GLLNTIWWLV VLYVLLNQAL AALLCHEFYH EKFDTYPIHR 300
    KAFIPFIF 308
    Length:308
    Mass (Da):35,876
    Last modified:September 19, 2006 - v1
    Checksum:iFFE931DCDB0D558A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122039 mRNA. Translation: AAI22040.1.
    RefSeqiNP_001072539.1. NM_001079071.1.
    UniGeneiStr.24988.

    Genome annotation databases

    GeneIDi779994.
    KEGGixtr:779994.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122039 mRNA. Translation: AAI22040.1 .
    RefSeqi NP_001072539.1. NM_001079071.1.
    UniGenei Str.24988.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8364.ENSXETP00000045522.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 779994.
    KEGGi xtr:779994.

    Organism-specific databases

    CTDi 79644.
    Xenbasei XB-GENE-985511. srd5a3.

    Phylogenomic databases

    eggNOGi NOG330066.
    HOVERGENi HBG057797.
    KOi K12345.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_210591. Androgen biosynthesis.
    REACT_211888. Synthesis of Dolichyl-phosphate.

    Gene expression databases

    Bgeei Q0P4J9.

    Family and domain databases

    InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: N6.
      Tissue: Ovary.

    Entry informationi

    Entry nameiPORED_XENTR
    AccessioniPrimary (citable) accession number: Q0P4J9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: September 19, 2006
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3