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Q0P4J9

- PORED_XENTR

UniProt

Q0P4J9 - PORED_XENTR

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Protein
Polyprenol reductase
Gene
srd5a3
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) By similarity.

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

Pathwayi

GO - Molecular functioni

  1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: UniProtKB
  2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
  3. oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Source: UniProtKB

GO - Biological processi

  1. dolichol metabolic process Source: UniProtKB
  2. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
  3. polyprenol catabolic process Source: UniProtKB
  4. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_210591. Androgen biosynthesis.
REACT_211888. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3 (EC:1.3.1.22)
Steroid 5-alpha-reductase 3
Short name:
S5AR 3
Short name:
SR type 3
Gene namesi
Name:srd5a3
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-985511. srd5a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22Cytoplasmic Reviewed prediction
Transmembranei3 – 2321Helical; Reviewed prediction
Add
BLAST
Topological domaini24 – 6542Lumenal Reviewed prediction
Add
BLAST
Transmembranei66 – 8621Helical; Reviewed prediction
Add
BLAST
Topological domaini87 – 12034Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei121 – 14121Helical; Reviewed prediction
Add
BLAST
Topological domaini142 – 1487Lumenal Reviewed prediction
Transmembranei149 – 16921Helical; Reviewed prediction
Add
BLAST
Topological domaini170 – 18415Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei185 – 20521Helical; Reviewed prediction
Add
BLAST
Topological domaini206 – 25550Lumenal Reviewed prediction
Add
BLAST
Transmembranei256 – 27621Helical; Reviewed prediction
Add
BLAST
Topological domaini277 – 30832Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Polyprenol reductase
PRO_0000317706Add
BLAST

Expressioni

Gene expression databases

BgeeiQ0P4J9.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000045522.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG330066.
HOVERGENiHBG057797.
KOiK12345.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0P4J9-1 [UniParc]FASTAAdd to Basket

« Hide

MTLLALVWLL LDATFLITLL WHLLQGCKSG HSLLCSVFQD LIRYGKTKTG    50
LQRPAWLQWF DIPKRCFWHF YCVSLIWNGC LLWILLRLLL QSVPVPEWLQ 100
LVLHFLHAGS EPQILDRELS VILALALLWL HSLRRLLECL FVSVFSNGVI 150
HLVQYCFGLG YYFLIGITVL TYCPLDRRTV STDNLLTQCH WYHILGLALY 200
IWASLHQYRC HCILAGLRKS ASGNVINLNH SVPCGDWFER VSCPHYFAEL 250
LIYVSIAVVF GLLNTIWWLV VLYVLLNQAL AALLCHEFYH EKFDTYPIHR 300
KAFIPFIF 308
Length:308
Mass (Da):35,876
Last modified:September 19, 2006 - v1
Checksum:iFFE931DCDB0D558A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC122039 mRNA. Translation: AAI22040.1.
RefSeqiNP_001072539.1. NM_001079071.1.
UniGeneiStr.24988.

Genome annotation databases

GeneIDi779994.
KEGGixtr:779994.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC122039 mRNA. Translation: AAI22040.1 .
RefSeqi NP_001072539.1. NM_001079071.1.
UniGenei Str.24988.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000045522.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 779994.
KEGGi xtr:779994.

Organism-specific databases

CTDi 79644.
Xenbasei XB-GENE-985511. srd5a3.

Phylogenomic databases

eggNOGi NOG330066.
HOVERGENi HBG057797.
KOi K12345.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_210591. Androgen biosynthesis.
REACT_211888. Synthesis of Dolichyl-phosphate.

Gene expression databases

Bgeei Q0P4J9.

Family and domain databases

InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view ]
Pfami PF02544. Steroid_dh. 1 hit.
[Graphical view ]
PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: N6.
    Tissue: Ovary.

Entry informationi

Entry nameiPORED_XENTR
AccessioniPrimary (citable) accession number: Q0P4J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 19, 2006
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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