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Q0P4J9 (PORED_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyprenol reductase

EC=1.3.1.94
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3
EC=1.3.1.22
Steroid 5-alpha-reductase 3
Short name=S5AR 3
Short name=SR type 3
Gene names
Name:srd5a3
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) By similarity.

Catalytic activity

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308Polyprenol reductase
PRO_0000317706

Regions

Topological domain1 – 22Cytoplasmic Potential
Transmembrane3 – 2321Helical; Potential
Topological domain24 – 6542Lumenal Potential
Transmembrane66 – 8621Helical; Potential
Topological domain87 – 12034Cytoplasmic Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 1487Lumenal Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 18415Cytoplasmic Potential
Transmembrane185 – 20521Helical; Potential
Topological domain206 – 25550Lumenal Potential
Transmembrane256 – 27621Helical; Potential
Topological domain277 – 30832Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
Q0P4J9 [UniParc].

Last modified September 19, 2006. Version 1.
Checksum: FFE931DCDB0D558A

FASTA30835,876
        10         20         30         40         50         60 
MTLLALVWLL LDATFLITLL WHLLQGCKSG HSLLCSVFQD LIRYGKTKTG LQRPAWLQWF 

        70         80         90        100        110        120 
DIPKRCFWHF YCVSLIWNGC LLWILLRLLL QSVPVPEWLQ LVLHFLHAGS EPQILDRELS 

       130        140        150        160        170        180 
VILALALLWL HSLRRLLECL FVSVFSNGVI HLVQYCFGLG YYFLIGITVL TYCPLDRRTV 

       190        200        210        220        230        240 
STDNLLTQCH WYHILGLALY IWASLHQYRC HCILAGLRKS ASGNVINLNH SVPCGDWFER 

       250        260        270        280        290        300 
VSCPHYFAEL LIYVSIAVVF GLLNTIWWLV VLYVLLNQAL AALLCHEFYH EKFDTYPIHR 


KAFIPFIF 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: N6.
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC122039 mRNA. Translation: AAI22040.1.
RefSeqNP_001072539.1. NM_001079071.1.
UniGeneStr.24988.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING8364.ENSXETP00000045522.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID779994.
KEGGxtr:779994.

Organism-specific databases

CTD79644.
XenbaseXB-GENE-985511. srd5a3.

Phylogenomic databases

eggNOGNOG330066.
HOVERGENHBG057797.
KOK12345.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ0P4J9.

Family and domain databases

InterProIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePORED_XENTR
AccessionPrimary (citable) accession number: Q0P4J9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 19, 2006
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways