ID   SYEM_DANRE              Reviewed;         503 AA.
AC   Q0P499;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 25.
DE   RecName: Full=Probable glutamyl-tRNA synthetase, mitochondrial;
DE            EC=6.1.1.17;
DE   AltName: Full=Glutamate--tRNA ligase;
DE            Short=GluRS;
DE   Flags: Precursor;
GN   Name=ears2; ORFNames=zgc:153247;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the attachment of glutamate to tRNA(Glu) in a
CC       two-step reaction: glutamate is first activated by ATP to form
CC       Glu-AMP and then transferred to the acceptor end of tRNA(Glu) (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + L-glutamate + tRNA(Glu) = AMP +
CC       diphosphate + L-glutamyl-tRNA(Glu).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; BC122204; AAI22205.1; -; mRNA.
DR   IPI; IPI00786651; -.
DR   RefSeq; NP_001038907.1; -.
DR   UniGene; Dr.83804; -.
DR   PRIDE; Q0P499; -.
DR   GeneID; 751732; -.
DR   KEGG; dre:751732; -.
DR   ZFIN; ZDB-GENE-060825-214; zgc:153247.
DR   HOVERGEN; Q0P499; -.
DR   BRENDA; 6.1.1.17; 96826.
DR   Bgee; Q0P499; -.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004818; F:glutamate-tRNA ligase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006424; P:glutamyl-tRNA aminoacylation; IEA:InterPro.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR004527; Glu-tRNA-synth_Ic_bac/mito.
DR   InterPro; IPR000924; Glu/Gln-tRNA-synth_Ic.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR10119; Glu_tRNA-synt_1c; 1.
DR   TIGRFAMs; TIGR00464; gltX_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW   Nucleotide-binding; Protein biosynthesis; RNA-binding;
KW   Transit peptide.
FT   TRANSIT       1     22       Mitochondrion (Potential).
FT   CHAIN        23    503       Probable glutamyl-tRNA synthetase,
FT                                mitochondrial.
FT                                /FTId=PRO_0000254564.
FT   NP_BIND     265    269       ATP (By similarity).
FT   REGION       21     23       Glutamate binding (By similarity).
FT   REGION      209    213       Glutamate binding (By similarity).
FT   MOTIF        26     34       "HIGH" region.
FT   MOTIF       265    269       "KMSKS" region.
FT   BINDING      31     31       ATP (By similarity).
FT   BINDING      57     57       Glutamate (By similarity).
FT   BINDING     227    227       Glutamate (By similarity).
FT   BINDING     230    230       ATP (By similarity).
SQ   SEQUENCE   503 AA;  57023 MW;  B5E856B44B303AD7 CRC64;
     MKILRGVSRQ MCTSRPEVRV RFAPSPTGFL HLGGLRTALY NFLFSRQRRG VFILRLEDTD
     QKRLVPGAAE HIEDMLEWAG IPPDESSRRG GDYGPYVQSE RLHLYTEAAS SLLNTGHAYY
     CFCSNQRLEL LKKEAQRSGH APRYDNRCRR LQPQQVEQKL AAGVPAVVRF KLHTGTEEFQ
     DLVFGWTGHA VGAVEGDPVI LKADGYPTYH LASVVDDHHM RISHVLRGCE WLISSAKHLQ
     LYRALRWTPP TYAHLPLLLN RDGSKLSKRQ GDIFLQSFRD RGVLPETLLD LVTHAGSGFS
     DNRMGRRLDE LIRDFNISKI TTHSALLDLD KLEEFSRLHL QRRIEDPQQC VWLCEELKQM
     VKHTHSSEIS AAAVLEPEYI ERVLQLRKGH ISSLQDLLSS THSYLWVRPR VSQTQLQSES
     AHAKDIATAV MQMVLAGGSL VSMERLSSEL KQISSRTNST HSSVMKVLRL LLSAQQRGPS
     VAEMMLSLGE QEVCVRLQKA LEL
//