Reviewed, UniProtKB/Swiss-Prot Q0P499 (SYEM_DANRE)

Names and origin

Protein names

Recommended name:
    Probable glutamyl-tRNA synthetase, mitochondrial
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase
      Short name=GluRS

Gene names

Name:	ears2
ORF Names:	zgc:153247

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 503	481	Probable glutamyl-tRNA synthetase, mitochondrial		PRO_0000254564
Regions
	Nucleotide binding	265 – 269	5	ATP By similarity
	Region	21 – 23	3	Glutamate binding By similarity
	Region	209 – 213	5	Glutamate binding By similarity
	Motif	26 – 34	9	"HIGH" region
	Motif	265 – 269	5	"KMSKS" region
Sites
	Binding site	31	1	ATP By similarity
	Binding site	57	1	Glutamate By similarity
	Binding site	227	1	Glutamate By similarity
	Binding site	230	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0P499-1 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: B5E856B44B303AD7	FASTA	503	57,023
	10 20 30 40 50 60 MKILRGVSRQ MCTSRPEVRV RFAPSPTGFL HLGGLRTALY NFLFSRQRRG VFILRLEDTD 70 80 90 100 110 120 QKRLVPGAAE HIEDMLEWAG IPPDESSRRG GDYGPYVQSE RLHLYTEAAS SLLNTGHAYY 130 140 150 160 170 180 CFCSNQRLEL LKKEAQRSGH APRYDNRCRR LQPQQVEQKL AAGVPAVVRF KLHTGTEEFQ 190 200 210 220 230 240 DLVFGWTGHA VGAVEGDPVI LKADGYPTYH LASVVDDHHM RISHVLRGCE WLISSAKHLQ 250 260 270 280 290 300 LYRALRWTPP TYAHLPLLLN RDGSKLSKRQ GDIFLQSFRD RGVLPETLLD LVTHAGSGFS 310 320 330 340 350 360 DNRMGRRLDE LIRDFNISKI TTHSALLDLD KLEEFSRLHL QRRIEDPQQC VWLCEELKQM 370 380 390 400 410 420 VKHTHSSEIS AAAVLEPEYI ERVLQLRKGH ISSLQDLLSS THSYLWVRPR VSQTQLQSES 430 440 450 460 470 480 AHAKDIATAV MQMVLAGGSL VSMERLSSEL KQISSRTNST HSSVMKVLRL LLSAQQRGPS 490 500 VAEMMLSLGE QEVCVRLQKA LEL

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC122204 mRNA. Translation: AAI22205.1.
IPI	IPI00786651.
RefSeq	NP_001038907.1.
UniGene	Dr.83804
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q0P499.
Genome annotation databases
GeneID	751732.
KEGG	dre:751732.
Organism-specific databases
ZFIN	ZDB-GENE-060825-214. zgc:153247.
Phylogenomic databases
HOVERGEN	Q0P499.
Enzyme and pathway databases
BRENDA	6.1.1.17. 96826.
Gene expression databases
Bgee	Q0P499.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYEM_DANRE

Accession

Primary (citable) accession number: Q0P499

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 19, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project