ID   NB5R2_DANRE             Reviewed;         309 AA.
AC   Q0P487; A2CET6;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 2.
DT   16-JUN-2009, entry version 29.
DE   RecName: Full=NADH-cytochrome b5 reductase 2;
DE            Short=b5R.2;
DE            EC=1.6.2.2;
GN   Name=cyb5r2; ORFNames=si:dkey-80c24.9, zgc:153291;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RG   The Danio rerio sequencing project at the Sanger Institute;
RL   Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB; TISSUE=Skin;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NADH-cytochrome b5 reductases are involved in
CC       desaturation and elongation of fatty acids, cholesterol
CC       biosynthesis and drug metabolism (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD(+) + H(+) +
CC       2 ferrocytochrome b5.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein
CC       (Potential).
CC   -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide
CC       cytochrome reductase family.
CC   -!- SIMILARITY: Contains 1 FAD-binding FR-type domain.
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DR   EMBL; CR759887; CAM16402.1; -; Genomic_DNA.
DR   EMBL; BC122221; AAI22222.1; -; mRNA.
DR   IPI; IPI00786645; -.
DR   RefSeq; NP_001038825.1; -.
DR   UniGene; Dr.77055; -.
DR   Ensembl; ENSDARG00000051925; Danio rerio.
DR   GeneID; 751641; -.
DR   KEGG; dre:751641; -.
DR   ZFIN; ZDB-GENE-060825-83; zgc:153291.
DR   HOVERGEN; Q0P487; -.
DR   OMA; Q0P487; GMNKAMA.
DR   BRENDA; 1.6.2.2; 96826.
DR   Bgee; Q0P487; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004128; F:cytochrome-b5 reductase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR017927; Fd_Rdtase_FAD-bd.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR001834; NADH-Cyt_B5_reductase.
DR   InterPro; IPR008333; OxRdtase_FAD-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD_bd.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00371; FPNCR.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Lipid synthesis; Membrane; NAD; Oxidoreductase;
KW   Steroid biosynthesis; Sterol biosynthesis; Transmembrane.
FT   CHAIN         1    309       NADH-cytochrome b5 reductase 2.
FT                                /FTId=PRO_0000287552.
FT   TRANSMEM      3     23       Potential.
FT   DOMAIN       48    160       FAD-binding FR-type.
FT   NP_BIND     140    170       FAD (By similarity).
FT   NP_BIND     179    214       FAD (By similarity).
FT   CONFLICT    199    199       I -> V (in Ref. 2; AAI22222).
FT   CONFLICT    207    207       F -> S (in Ref. 2; AAI22222).
SQ   SEQUENCE   309 AA;  34388 MW;  9EE4889EE66784F9 CRC64;
     MDILTAPVLI GVSIVVITVL YLFLKPAGSN STPPKPQNKI PKALQDPSVK YPLPLIEKEE
     INHDTKRFRF GLPSSSHVLG LPIGQHIYLS AKVNGSLVVR AYTPVSSDQD QGYVDLVVKV
     YYKNTHPSYP DGGKMSQYLD NMKIGDTIDF RGPNGLLVYN GKGKFAIRPD KKSEAEVRKF
     KHVAMIAGGT GITPMLQLIR SITADSFDET VCSLIFANQT EKDILLRNEL DEVHRNHPSK
     LKLWYTLDRP SEGWKYSEGF VNAAMMKDHL PPADSDVLVV MCGPPAMIEK ACLPNLLKLG
     YKKENIFAY
//