Q0P487 (NB5R2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 2
Short name=b5R.2
EC=1.6.2.2

Gene names

Name:	cyb5r2
ORF Names:	si:dkey-80c24.9, zgc:153291

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis and drug metabolism By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	NADH-cytochrome b5 reductase 2		PRO_0000287552
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Domain	48 – 160	113	FAD-binding FR-type
	Nucleotide binding	140 – 170	31	FAD By similarity
	Nucleotide binding	179 – 214	36	FAD By similarity
Experimental info
	Sequence conflict	199	1	I → V in AAI22222. Ref.2
	Sequence conflict	207	1	F → S in AAI22222. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q0P487 [UniParc]. Last modified May 15, 2007. Version 2. Checksum: 9EE4889EE66784F9	FASTA	309	34,388
	10 20 30 40 50 60 MDILTAPVLI GVSIVVITVL YLFLKPAGSN STPPKPQNKI PKALQDPSVK YPLPLIEKEE 70 80 90 100 110 120 INHDTKRFRF GLPSSSHVLG LPIGQHIYLS AKVNGSLVVR AYTPVSSDQD QGYVDLVVKV 130 140 150 160 170 180 YYKNTHPSYP DGGKMSQYLD NMKIGDTIDF RGPNGLLVYN GKGKFAIRPD KKSEAEVRKF 190 200 210 220 230 240 KHVAMIAGGT GITPMLQLIR SITADSFDET VCSLIFANQT EKDILLRNEL DEVHRNHPSK 250 260 270 280 290 300 LKLWYTLDRP SEGWKYSEGF VNAAMMKDHL PPADSDVLVV MCGPPAMIEK ACLPNLLKLG YKKENIFAY

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB. Tissue: Skin.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR759887 Genomic DNA. Translation: CAM16402.1. BC122221 mRNA. Translation: AAI22222.1.
IPI	IPI00786645.
RefSeq	NP_001038825.1. NM_001045360.1.
UniGene	Dr.77055.
3D structure databases
ProteinModelPortal	Q0P487.
SMR	Q0P487. Positions 44-309.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0P487.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000090388; ENSDARP00000084821; ENSDARG00000051925.
GeneID	751641.
KEGG	dre:751641.
Organism-specific databases
CTD	51700.
ZFIN	ZDB-GENE-060825-83. cyb5r2.
Phylogenomic databases
eggNOG	fiNOG09399.
GeneTree	ENSGT00390000008881.
HOGENOM	HBG591994.
HOVERGEN	HBG052580.
InParanoid	Q0P487.
OMA	YLTANID.
OrthoDB	EOG4WDDCG.
PhylomeDB	Q0P487.
Gene expression databases
Bgee	Q0P487.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
KO	K00326.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NB5R2_DANRE

Accession

Primary (citable) accession number: Q0P487
Secondary accession number(s): A2CET6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	November 16, 2011
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program