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Reviewed, UniProtKB/Swiss-Prot Q0P487 (NB5R2_DANRE)

Last modified January 19, 2010. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADH-cytochrome b5 reductase 2
      Short name=b5R.2
    EC=1.6.2.2
Gene names
Name: cyb5r2
ORF Names: si:dkey-80c24.9, zgc:153291
OrganismDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

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Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis and drug metabolism By similarity.

Catalytic activity

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactor

FAD By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Contains 1 FAD-binding FR-type domain.

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Ontologies

Keywords
   Biological processLipid synthesis
Steroid biosynthesis
Sterol biosynthesis
   Cellular componentMembrane
   DomainTransmembrane
   LigandFAD
Flavoprotein
NAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

sterol biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncytochrome-b5 reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309NADH-cytochrome b5 reductase 2
PRO_0000287552

Regions

Transmembrane3 – 2321 Potential
Domain48 – 160113FAD-binding FR-type
Nucleotide binding140 – 17031FAD By similarity
Nucleotide binding179 – 21436FAD By similarity

Experimental info

Sequence conflict1991I → V in AAI22222. Ref.2
Sequence conflict2071F → S in AAI22222. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q0P487-1 [UniParc].

Last modified May 15, 2007. Version 2.
Checksum: 9EE4889EE66784F9

FASTA30934,388
        10         20         30         40         50         60 
MDILTAPVLI GVSIVVITVL YLFLKPAGSN STPPKPQNKI PKALQDPSVK YPLPLIEKEE 

        70         80         90        100        110        120 
INHDTKRFRF GLPSSSHVLG LPIGQHIYLS AKVNGSLVVR AYTPVSSDQD QGYVDLVVKV 

       130        140        150        160        170        180 
YYKNTHPSYP DGGKMSQYLD NMKIGDTIDF RGPNGLLVYN GKGKFAIRPD KKSEAEVRKF 

       190        200        210        220        230        240 
KHVAMIAGGT GITPMLQLIR SITADSFDET VCSLIFANQT EKDILLRNEL DEVHRNHPSK 

       250        260        270        280        290        300 
LKLWYTLDRP SEGWKYSEGF VNAAMMKDHL PPADSDVLVV MCGPPAMIEK ACLPNLLKLG 


YKKENIFAY

« Hide

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References

[1]The Danio rerio sequencing project at the Sanger Institute
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tuebingen.
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AB.
Tissue: Skin.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR759887 Genomic DNA. Translation: CAM16402.1.
BC122221 mRNA. Translation: AAI22222.1.
IPIIPI00786645.
RefSeqNP_001038825.1.
UniGeneDr.77055

3D structure databases

SMRQ0P487. Positions 44-309.
ModBaseSearch...

Genome annotation databases

EnsemblENSDART00000090388; ENSDARP00000084821; ENSDARG00000051925; Danio rerio. [Genome view]
GeneID751641.
KEGGdre:751641.

Organism-specific databases

CTD751641.
ZFINZDB-GENE-060825-83. cyb5r2.

Phylogenomic databases

eggNOGfiNOG09399.
HOGENOMHBG591994.
HOVERGENQ0P487.
InParanoidQ0P487.
OMAARFKFAL.
OrthoDBEOG9KM1NT.
PhylomeDBQ0P487.

Enzyme and pathway databases

BRENDA1.6.2.2. 96826.

Gene expression databases

BgeeQ0P487.

Family and domain databases

InterProIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD_bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSPR00406. CYTB5RDTASE.
PR00371. FPNCR.
PROSITEPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNB5R2_DANRE
AccessionPrimary (citable) accession number: Q0P487
Secondary accession number(s): A2CET6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: January 19, 2010
This is version 36 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectZebrafish annotation project

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents