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Protein

Ribulose bisphosphate carboxylase large chain

Gene

rbcL

Organism
Ostreococcus tauri
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231Substrate; in homodimeric partnerUniRule annotation
Binding sitei173 – 1731SubstrateUniRule annotation
Active sitei175 – 1751Proton acceptorUniRule annotation
Binding sitei177 – 1771SubstrateUniRule annotation
Metal bindingi201 – 2011Magnesium; via carbamate groupUniRule annotation
Metal bindingi203 – 2031MagnesiumUniRule annotation
Metal bindingi204 – 2041MagnesiumUniRule annotation
Active sitei294 – 2941Proton acceptorUniRule annotation
Binding sitei295 – 2951SubstrateUniRule annotation
Binding sitei327 – 3271SubstrateUniRule annotation
Sitei334 – 3341Transition state stabilizerUniRule annotation
Binding sitei379 – 3791SubstrateUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. monooxygenase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. photorespiration Source: UniProtKB-KW
  2. reductive pentose-phosphate cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Calvin cycle, Carbon dioxide fixation, Photorespiration, Photosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:OtCpg00590
Encoded oniPlastid; Chloroplast
OrganismiOstreococcus tauri
Taxonomic identifieri70448 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaprasinophytesMamiellophyceaeMamiellalesBathycoccaceaeOstreococcus
ProteomesiUP000009170 Componenti: Chloroplast

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 22UniRule annotationPRO_0000251427
Chaini3 – 475473Ribulose bisphosphate carboxylase large chainPRO_0000251428Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31N-acetylprolineUniRule annotation
Modified residuei201 – 2011N6-carboxylysineUniRule annotation

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ0P3J3.

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

Protein-protein interaction databases

STRINGi70448.Q0P3J3.

Structurei

3D structure databases

ProteinModelPortaliQ0P3J3.
SMRiQ0P3J3. Positions 12-470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1850.
HOGENOMiHOG000230831.
InParanoidiQ0P3J3.
KOiK01601.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0P3J3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPQTETKTG TGFQAGVKDY RLTYYTPDYQ VKETDILAAF RMTPQPGVPA
60 70 80 90 100
EECGAAVAAE SSTGTWTTVW TDGLTQLDRY KGRCYDLEPV PGEDNQFIAY
110 120 130 140 150
VAYPLDLFEE GSVTNLFTSI VGNVFGFKAL RALRLEDLRI PVAYCKTFQG
160 170 180 190 200
APHGIQTERD KLNKYGRGLL GCTIKPKLGL SAKNYGRAVY ECLRGGLDFT
210 220 230 240 250
KDDENVNSQP FMRWRDRFLF VAEAIYKSQA ETGEIKGHYL NATAGNVDQM
260 270 280 290 300
LKRAQVAKEL GMPIIMHDYL TAGFTANTTL ATYCREEGLL LHIHRAMHAV
310 320 330 340 350
IDRQRNHGIH FRVLAKALRL SGGDHLHSGT VVGKLEGERN VTLGFVDLMR
360 370 380 390 400
DAYVEKDRDR GIYFSQDWAS LPGVMPVASG GIHVWHMPAL VEIFGDDACL
410 420 430 440 450
QFGGGTLGHP WGNAPGASAN RVALEACTQA RNEGRDLARE GGDVIRAACK
460 470
WSPELAAACE VWKEIKFEFD TVDTL
Length:475
Mass (Da):52,520
Last modified:September 19, 2006 - v1
Checksum:i90EFD83EA2992B11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954199 Genomic DNA. Translation: CAL36384.1.
RefSeqiYP_717262.1. NC_008289.1.

Genome annotation databases

GeneIDi4238788.
KEGGiota:OstapCp59.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954199 Genomic DNA. Translation: CAL36384.1.
RefSeqiYP_717262.1. NC_008289.1.

3D structure databases

ProteinModelPortaliQ0P3J3.
SMRiQ0P3J3. Positions 12-470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70448.Q0P3J3.

Proteomic databases

PRIDEiQ0P3J3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4238788.
KEGGiota:OstapCp59.

Phylogenomic databases

eggNOGiCOG1850.
HOGENOMiHOG000230831.
InParanoidiQ0P3J3.
KOiK01601.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete chloroplast and mitochondrial DNA sequence of Ostreococcus tauri: organelle genomes of the smallest eukaryote are examples of compaction."
    Robbens S., Derelle E., Ferraz C., Wuyts J., Moreau H., Van de Peer Y.
    Mol. Biol. Evol. 24:956-968(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OTTH0595.

Entry informationi

Entry nameiRBL_OSTTA
AccessioniPrimary (citable) accession number: Q0P3J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: September 19, 2006
Last modified: January 7, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.