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Protein

Ribulose bisphosphate carboxylase large chain

Gene

rbcL

Organism
Ostreococcus tauri
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123Substrate; in homodimeric partnerUniRule annotation1
Binding sitei173SubstrateUniRule annotation1
Active sitei175Proton acceptorUniRule annotation1
Binding sitei177SubstrateUniRule annotation1
Metal bindingi201Magnesium; via carbamate groupUniRule annotation1
Metal bindingi203MagnesiumUniRule annotation1
Metal bindingi204MagnesiumUniRule annotation1
Active sitei294Proton acceptorUniRule annotation1
Binding sitei295SubstrateUniRule annotation1
Binding sitei327SubstrateUniRule annotation1
Sitei334Transition state stabilizerUniRule annotation1
Binding sitei379SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Calvin cycle, Carbon dioxide fixation, Photorespiration, Photosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:OtCpg00590
Encoded oniPlastid; Chloroplast
OrganismiOstreococcus tauri
Taxonomic identifieri70448 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaprasinophytesMamiellophyceaeMamiellalesBathycoccaceaeOstreococcus
Proteomesi
  • UP000009170 Componenti: Chloroplast

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00002514271 – 2UniRule annotation2
ChainiPRO_00002514283 – 475Ribulose bisphosphate carboxylase large chainAdd BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3N-acetylprolineUniRule annotation1
Modified residuei201N6-carboxylysineUniRule annotation1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ0P3J3.

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

Protein-protein interaction databases

STRINGi70448.Q0P3J3.

Structurei

3D structure databases

ProteinModelPortaliQ0P3J3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410IIVP. Eukaryota.
COG1850. LUCA.
HOGENOMiHOG000230831.
InParanoidiQ0P3J3.
KOiK01601.

Family and domain databases

CDDicd08212. RuBisCO_large_I. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0P3J3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPQTETKTG TGFQAGVKDY RLTYYTPDYQ VKETDILAAF RMTPQPGVPA
60 70 80 90 100
EECGAAVAAE SSTGTWTTVW TDGLTQLDRY KGRCYDLEPV PGEDNQFIAY
110 120 130 140 150
VAYPLDLFEE GSVTNLFTSI VGNVFGFKAL RALRLEDLRI PVAYCKTFQG
160 170 180 190 200
APHGIQTERD KLNKYGRGLL GCTIKPKLGL SAKNYGRAVY ECLRGGLDFT
210 220 230 240 250
KDDENVNSQP FMRWRDRFLF VAEAIYKSQA ETGEIKGHYL NATAGNVDQM
260 270 280 290 300
LKRAQVAKEL GMPIIMHDYL TAGFTANTTL ATYCREEGLL LHIHRAMHAV
310 320 330 340 350
IDRQRNHGIH FRVLAKALRL SGGDHLHSGT VVGKLEGERN VTLGFVDLMR
360 370 380 390 400
DAYVEKDRDR GIYFSQDWAS LPGVMPVASG GIHVWHMPAL VEIFGDDACL
410 420 430 440 450
QFGGGTLGHP WGNAPGASAN RVALEACTQA RNEGRDLARE GGDVIRAACK
460 470
WSPELAAACE VWKEIKFEFD TVDTL
Length:475
Mass (Da):52,520
Last modified:September 19, 2006 - v1
Checksum:i90EFD83EA2992B11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954199 Genomic DNA. Translation: CAL36384.1.
RefSeqiYP_717262.1. NC_008289.1.

Genome annotation databases

GeneIDi4238788.
KEGGiota:OstapCp59.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR954199 Genomic DNA. Translation: CAL36384.1.
RefSeqiYP_717262.1. NC_008289.1.

3D structure databases

ProteinModelPortaliQ0P3J3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70448.Q0P3J3.

Proteomic databases

PRIDEiQ0P3J3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4238788.
KEGGiota:OstapCp59.

Phylogenomic databases

eggNOGiENOG410IIVP. Eukaryota.
COG1850. LUCA.
HOGENOMiHOG000230831.
InParanoidiQ0P3J3.
KOiK01601.

Family and domain databases

CDDicd08212. RuBisCO_large_I. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBL_OSTTA
AccessioniPrimary (citable) accession number: Q0P3J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: September 19, 2006
Last modified: November 30, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.