Reviewed, UniProtKB/Swiss-Prot Q0NXR6 (ACAD8_BOVIN)

Names and origin

Protein names

Recommended name:
    Isobutyryl-CoA dehydrogenase, mitochondrial
    EC=1.3.99.-
Alternative name(s):
    Acyl-CoA dehydrogenase family member 8
      Short name=ACAD-8

Gene names

Name:

ACAD8

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has very high activity toward isobutyryl-CoA. Is an isobutyryl-CoA dehydrogenase that functions in valine catabolism By similarity.
Catalytic activity	Isobutyryl-CoA + ETF = methylacrylyl-CoA + reduced ETF.
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; L-valine degradation.
Subunit structure	Homotetramer, formed by a dimer of dimers. Subunit of the large multiprotein complex ARC/DRIP By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the acyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Branched-chain amino acid catabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	branched chain family amino acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 23	23	Mitochondrion Potential
	Chain	24 – 416	393	Isobutyryl-CoA dehydrogenase, mitochondrial		PRO_0000253024
Regions
	Nucleotide binding	158 – 168	11	FAD By similarity
	Nucleotide binding	372 – 378	7	FAD By similarity
	Nucleotide binding	401 – 403	3	FAD By similarity
	Region	275 – 278	4	Substrate binding By similarity
Sites
	Active site	399	1	Proton acceptor By similarity
	Binding site	303	1	FAD By similarity
	Binding site	411	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0NXR6-1 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: 86BE48848C2C92DC	FASTA	416	45,307
	10 20 30 40 50 60 MMLRGGCQRV GARLRGLRRG PRGPADGARR GVVSCIDPSM GLSEEQKEFQ KVAFNFAARE 70 80 90 100 110 120 MAPHMAEWDQ KELFPVDTMR KAAQLGFGGV YVQTDVGGAG LSRLDTSIIF EALATGCTST 130 140 150 160 170 180 TAYMSIHNMC VWIIDRFGSE EQRHRLCPPL CTMEKFASYC LTEPGSGSDA ASLMTSAVRQ 190 200 210 220 230 240 HDHYILNGSK AFISGGGEAD IYVVMCRTGG PGPRGISCVV VEKGTPGLSF GKKEKKVGWN 250 260 270 280 290 300 SQPTQAVIFE DCAVPVANRI GDEGQGFLIA MKGLNGGRIN VASCSLGAAH ASIVLARDYL 310 320 330 340 350 360 KVRKQFGEPL ANSQYLQFQL ADMAARLVAS RLMIRTAATA LQEEREDAIV LCSMAKLFAT 370 380 390 400 410 DECFAICNQA LQMHGGYGYL KDYAVQQYVR DSRVHQILEG SNEVMRMLIS RSLLQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the bovine ACAD8 gene and the association of its polymorphism with the production traits." Li H., Xu S., Gao X., Ren H. J. Genet. Genomics 34:315-320(2007) [PubMed: 17498629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ435444 mRNA. Translation: ABD97096.1. DQ435445 Genomic DNA. Translation: ABD97097.1. BC126750 mRNA. Translation: AAI26751.1.
IPI	IPI00711963.
RefSeq	NP_001069019.1.
UniGene	Bt.36400
3D structure databases
SMR	Q0NXR6. Positions 34-416.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000012937. Bos taurus. [Contig view]
GeneID	512070.
KEGG	bta:512070.
Phylogenomic databases
HOVERGEN	Q0NXR6.
Family and domain databases
InterPro	IPR006089. Acyl-CoA_DH_CS. IPR006092. Acyl-CoA_DH_N. IPR006090. Acyl-CoA_Oxase/DH_1. IPR006091. Acyl-CoA_Oxase/DH_M. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view]
Gene3D	G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.
Pfam	PF00441. Acyl-CoA_dh_1. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. PF02771. Acyl-CoA_dh_N. 1 hit. [Graphical view]
PROSITE	PS00072. ACYL_COA_DH_1. 1 hit. PS00073. ACYL_COA_DH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACAD8_BOVIN

Accession

Primary (citable) accession number: Q0NXR6
Secondary accession number(s): A1A4N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	September 19, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)