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Protein

Isobutyryl-CoA dehydrogenase, mitochondrial

Gene

ACAD8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has very high activity toward isobutyryl-CoA. Is an isobutyryl-CoA dehydrogenase that functions in valine catabolism (By similarity).By similarity

Catalytic activityi

Isobutyryl-CoA + ETF = methylacrylyl-CoA + reduced ETF.

Cofactori

FADBy similarity

Pathwayi: L-valine degradation

This protein is involved in the pathway L-valine degradation, which is part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the pathway L-valine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei168 – 1681Substrate; via carbonyl oxygenBy similarity
Binding sitei303 – 3031FADBy similarity
Binding sitei303 – 3031FAD; shared with dimeric partnerBy similarity
Active sitei399 – 3991Proton acceptorBy similarity
Binding sitei400 – 4001Substrate; via amide nitrogenBy similarity
Binding sitei411 – 4111SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi158 – 16811FADBy similarityAdd
BLAST
Nucleotide bindingi159 – 16810FADBy similarity
Nucleotide bindingi192 – 1943FADBy similarity
Nucleotide bindingi313 – 3142FAD; shared with dimeric partnerBy similarity
Nucleotide bindingi372 – 3787FADBy similarity
Nucleotide bindingi401 – 4033FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Branched-chain amino acid catabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00362.

Names & Taxonomyi

Protein namesi
Recommended name:
Isobutyryl-CoA dehydrogenase, mitochondrial (EC:1.3.99.-)
Alternative name(s):
Acyl-CoA dehydrogenase family member 8
Short name:
ACAD-8
Gene namesi
Name:ACAD8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2323MitochondrionSequence analysisAdd
BLAST
Chaini24 – 416393Isobutyryl-CoA dehydrogenase, mitochondrialPRO_0000253024Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511N6-acetyllysine; alternateBy similarity
Modified residuei51 – 511N6-succinyllysine; alternateBy similarity
Modified residuei232 – 2321N6-acetyllysineBy similarity
Modified residuei272 – 2721N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ0NXR6.
PRIDEiQ0NXR6.

Interactioni

Subunit structurei

Homotetramer, formed by a dimer of dimers. Subunit of the large multiprotein complex ARC/DRIP (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017188.

Structurei

3D structure databases

ProteinModelPortaliQ0NXR6.
SMRiQ0NXR6. Positions 34-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni275 – 2784Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0140. Eukaryota.
COG1960. LUCA.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ0NXR6.
KOiK11538.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0NXR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLRGGCQRV GARLRGLRRG PRGPADGARR GVVSCIDPSM GLSEEQKEFQ
60 70 80 90 100
KVAFNFAARE MAPHMAEWDQ KELFPVDTMR KAAQLGFGGV YVQTDVGGAG
110 120 130 140 150
LSRLDTSIIF EALATGCTST TAYMSIHNMC VWIIDRFGSE EQRHRLCPPL
160 170 180 190 200
CTMEKFASYC LTEPGSGSDA ASLMTSAVRQ HDHYILNGSK AFISGGGEAD
210 220 230 240 250
IYVVMCRTGG PGPRGISCVV VEKGTPGLSF GKKEKKVGWN SQPTQAVIFE
260 270 280 290 300
DCAVPVANRI GDEGQGFLIA MKGLNGGRIN VASCSLGAAH ASIVLARDYL
310 320 330 340 350
KVRKQFGEPL ANSQYLQFQL ADMAARLVAS RLMIRTAATA LQEEREDAIV
360 370 380 390 400
LCSMAKLFAT DECFAICNQA LQMHGGYGYL KDYAVQQYVR DSRVHQILEG
410
SNEVMRMLIS RSLLQE
Length:416
Mass (Da):45,307
Last modified:September 19, 2006 - v1
Checksum:i86BE48848C2C92DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ435444 mRNA. Translation: ABD97096.1.
DQ435445 Genomic DNA. Translation: ABD97097.1.
BC126750 mRNA. Translation: AAI26751.1.
RefSeqiNP_001069019.1. NM_001075551.1.
UniGeneiBt.36400.

Genome annotation databases

GeneIDi512070.
KEGGibta:512070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ435444 mRNA. Translation: ABD97096.1.
DQ435445 Genomic DNA. Translation: ABD97097.1.
BC126750 mRNA. Translation: AAI26751.1.
RefSeqiNP_001069019.1. NM_001075551.1.
UniGeneiBt.36400.

3D structure databases

ProteinModelPortaliQ0NXR6.
SMRiQ0NXR6. Positions 34-416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017188.

Proteomic databases

PaxDbiQ0NXR6.
PRIDEiQ0NXR6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi512070.
KEGGibta:512070.

Organism-specific databases

CTDi27034.

Phylogenomic databases

eggNOGiKOG0140. Eukaryota.
COG1960. LUCA.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ0NXR6.
KOiK11538.

Enzyme and pathway databases

UniPathwayiUPA00362.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the bovine ACAD8 gene and the association of its polymorphism with the production traits."
    Li H., Xu S., Gao X., Ren H.
    J. Genet. Genomics 34:315-320(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal muscle.

Entry informationi

Entry nameiACAD8_BOVIN
AccessioniPrimary (citable) accession number: Q0NXR6
Secondary accession number(s): A1A4N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: September 19, 2006
Last modified: June 8, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.