Reviewed, UniProtKB/Swiss-Prot Q0KHT7 (Y2666_DROME)

Names and origin

Protein names

Recommended name:
    Probable serine/threonine-protein kinase CG32666
    EC=2.7.11.1

Gene names

ORF Names:

CG32666

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	784 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from mutant phenotype. Source: FlyBase protein amino acid phosphorylation Non-traceable author statement. Source: FlyBase regulation of cell shape Inferred from mutant phenotype. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Non-traceable author statement. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q0KHT7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q0KHT7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     671-674: AAMA → GIIC
     675-784: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 784	784	Probable serine/threonine-protein kinase CG32666		PRO_0000355636
Regions
	Domain	37 – 295	259	Protein kinase
	Nucleotide binding	43 – 51	9	ATP By similarity
	Compositional bias	314 – 319	6	Poly-Glu
	Compositional bias	326 – 332	7	Poly-Glu
	Compositional bias	335 – 340	6	Poly-Glu
	Compositional bias	353 – 357	5	Poly-Gln
	Compositional bias	512 – 629	118	Ser-rich
	Compositional bias	632 – 649	18	Poly-His
	Compositional bias	675 – 682	8	Poly-Thr
	Compositional bias	683 – 687	5	Poly-Pro
	Compositional bias	730 – 774	45	Trp-rich
Sites
	Active site	160	1	Proton acceptor By similarity
	Binding site	66	1	ATP By similarity
Amino acid modifications
	Modified residue	384	1	Phosphoserine Ref.4
	Modified residue	387	1	Phosphoserine Ref.4
	Modified residue	435	1	Phosphoserine Ref.4
	Modified residue	437	1	Phosphoserine Ref.4
	Modified residue	521	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	671 – 674	4	AAMA → GIIC in isoform 2.		VSP_035954
	Alternative sequence	675 – 784	110	Missing in isoform 2.		VSP_035955

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: AFA16D8D6B55A1B9	FASTA	784	85,684
	10 20 30 40 50 60 MFTEKGIFPI GDGLLDVDAD RLKGLLVSCP DINEIYEVEQ TPFARGKFAA VRRAIHKNTG 70 80 90 100 110 120 SHFAAKFLKR RRRAQSSDKE IKHEIAVLML CEGEDNIVNL NAVHETRSDT ALLLELATGG 130 140 150 160 170 180 ELQTILDNEE CLTEAQARHC MREVLKALKF LHDRSIAHLD LKPQNILLAG ERIEDGLKLC 190 200 210 220 230 240 DFGISRVVCE GINVREMAGT PDYVAPEVLQ YEPLSLLTDI WSVGVLTYVL LSGFSPFGGD 250 260 270 280 290 300 TKQETFLNIS QCALTFPDNL FGGVSPVAID FIRRALRIKP NDRMNATGCL DHIWLKDDCS 310 320 330 340 350 360 LDRQIYLQPQ SDAEEEEEED VDDDVEDEEE EEQVEEEEEE TQNVEEPTTE APQQQQQPVQ 370 380 390 400 410 420 QHQLAKSGQI HSKPTHNGHH RANSGGSISK IPIATSKLQS TPSSETTTAI HTLTSNSNGH 430 440 450 460 470 480 VHKPTQIVTP TRRASDSDKE NTYTATFVKK PVATIQLGSS NGIEDATVVA TLTLFPDAPT 490 500 510 520 530 540 TPKVIRKTPT GESNGSATSV KALVKKFQLE DSSGSAVARR SGGAVTSSSG LHSPTTTSVR 550 560 570 580 590 600 LNSIRRASEP LTTVYKKQTS QNGCSSTSNP SSSPGSSPTT SGSTATLLIH SQRLGNSTAP 610 620 630 640 650 660 ASHCVAVPAT ATATSSASSS NSSSGKSTSA AHHLHHHHMH HHHHHHHHHV VIAAKNAAAV 670 680 690 700 710 720 AATNNLSLDQ AAMATTTTTN TTPPPPPTSA HWQKAKRSGC ESPSCRITRW QSGDLRSYGY 730 740 750 760 770 780 GKGSALGSRW SWNGIGNGNG NLNLNWNWKW SWNWCCSGRW KWKWKWRGDH PPDWHEDDDA LILI
	Isoform 2. Checksum: BB94555F5161DBF5	FASTA	674	73,001
	10 20 30 40 50 60 MFTEKGIFPI GDGLLDVDAD RLKGLLVSCP DINEIYEVEQ TPFARGKFAA VRRAIHKNTG 70 80 90 100 110 120 SHFAAKFLKR RRRAQSSDKE IKHEIAVLML CEGEDNIVNL NAVHETRSDT ALLLELATGG 130 140 150 160 170 180 ELQTILDNEE CLTEAQARHC MREVLKALKF LHDRSIAHLD LKPQNILLAG ERIEDGLKLC 190 200 210 220 230 240 DFGISRVVCE GINVREMAGT PDYVAPEVLQ YEPLSLLTDI WSVGVLTYVL LSGFSPFGGD 250 260 270 280 290 300 TKQETFLNIS QCALTFPDNL FGGVSPVAID FIRRALRIKP NDRMNATGCL DHIWLKDDCS 310 320 330 340 350 360 LDRQIYLQPQ SDAEEEEEED VDDDVEDEEE EEQVEEEEEE TQNVEEPTTE APQQQQQPVQ 370 380 390 400 410 420 QHQLAKSGQI HSKPTHNGHH RANSGGSISK IPIATSKLQS TPSSETTTAI HTLTSNSNGH 430 440 450 460 470 480 VHKPTQIVTP TRRASDSDKE NTYTATFVKK PVATIQLGSS NGIEDATVVA TLTLFPDAPT 490 500 510 520 530 540 TPKVIRKTPT GESNGSATSV KALVKKFQLE DSSGSAVARR SGGAVTSSSG LHSPTTTSVR 550 560 570 580 590 600 LNSIRRASEP LTTVYKKQTS QNGCSSTSNP SSSPGSSPTT SGSTATLLIH SQRLGNSTAP 610 620 630 640 650 660 ASHCVAVPAT ATATSSASSS NSSSGKSTSA AHHLHHHHMH HHHHHHHHHV VIAAKNAAAV 670 AATNNLSLDQ GIIC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Berkeley. Tissue: Embryo.
[4]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384; SER-387; SER-435; SER-437 AND SER-521, MASS SPECTROMETRY. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014298 Genomic DNA. Translation: AAS65317.1. BT003530 mRNA. Translation: AAO39534.1.
RefSeq	NP_727533.1. NP_996411.1.
UniGene	Dm.17507
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q0KHT7.
Genome annotation databases
Ensembl	FBgn0052666. Drosophila melanogaster. [Contig view]
GeneID	32097.
KEGG	dme:Dmel_CG32666.
Organism-specific databases
FlyBase	FBgn0052666. CG32666.
Phylogenomic databases
OMA	Q0KHT7. SINIDIP.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	776818.

Entry information

Entry name

Y2666_DROME

Accession

Primary (citable) accession number: Q0KHT7
Secondary accession number(s): Q86P19

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	October 3, 2006
Last modified:	July 7, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project