Q0KFB0 (SYR_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	H16_A0159

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_1000018099
Regions
	Motif	132 – 142	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	Q0KFB0 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 22595641761F6198	FASTA	595	64,725
	10 20 30 40 50 60 MLPVQTSNLA AAFTDAVRAL APADATLPAV TFERPKVAAH GDLACNVAMQ VARALKSNPR 70 80 90 100 110 120 ELAQRIVDAV QADTRAQGLV QAMEIAGPGF INLRLTPAAK ADVLRAVLAE GDHYGARERG 130 140 150 160 170 180 VHGQVLVEFV SANPTGPLHV GHGRQAALGD ALANLLSWQG WHVHREFYYN DAGVQIQTLA 190 200 210 220 230 240 LSVQARARGL KPGDASWPEA AYNGDYIADI AADFLAGKTV SASDGEPVTA SGNVEDIDSI 250 260 270 280 290 300 RKFAVTYLRN EQDIDLQAFG VKFDRYYLES SLYSDGRVEA AVQSLVGKGK TYESEGALWL 310 320 330 340 350 360 RTTDDGDDKD RVMKKSDGTY TYFVPDVAYH TTKWERGFTK VINVQGSDHH GTIARVRAGL 370 380 390 400 410 420 QGLDMGIPQG YPDYVLHKMV TVMKNGEEVK ISKRAGSYVT VRDLIEWSNG GDETIRGCLD 430 440 450 460 470 480 TGVADWPQHF TRGRDAVRFF LLSRKADTEF VFDVDLALKQ NDENPVYYVQ YAHARICSIF 490 500 510 520 530 540 ESWGGADWEA RLAELAGADL SAVTGPEASA QAQALGRRLA EFPDMLSGAA AELAPHAVAF 550 560 570 580 590 YLRDLAGDFH AFYNADRVLV DDEAVKRARL ALLAATRQVL RNGLAVIGVS APRRM

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260479 Genomic DNA. Translation: CAJ91311.1.
RefSeq	YP_724679.1. NC_008313.1.
3D structure databases
ProteinModelPortal	Q0KFB0.
ModBase	Search...
Protein-protein interaction databases
STRING	381666.H16_A0159.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ91311; CAJ91311; H16_A0159.
GeneID	4247114.
KEGG	reh:H16_A0159.
PATRIC	35229679. VBIRalEut6770_0511.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247214.
KO	K01887.
OMA	ETIRGCL.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	CNEC381666:GJUJ-159-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_CUPNH

Accession

Primary (citable) accession number: Q0KFB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program