Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q0KFB0 (SYR_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arginine--tRNA ligase

EC=6.1.1.19
Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS
Gene names
Name:argS
Ordered Locus Names:H16_A0159
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length595 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00123

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00123.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

arginine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 595595Arginine--tRNA ligase HAMAP-Rule MF_00123
PRO_1000018099

Regions

Motif132 – 14211"HIGH" region HAMAP-Rule MF_00123

Sequences

Sequence LengthMass (Da)Tools
Q0KFB0 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 22595641761F6198

FASTA59564,725
        10         20         30         40         50         60 
MLPVQTSNLA AAFTDAVRAL APADATLPAV TFERPKVAAH GDLACNVAMQ VARALKSNPR 

        70         80         90        100        110        120 
ELAQRIVDAV QADTRAQGLV QAMEIAGPGF INLRLTPAAK ADVLRAVLAE GDHYGARERG 

       130        140        150        160        170        180 
VHGQVLVEFV SANPTGPLHV GHGRQAALGD ALANLLSWQG WHVHREFYYN DAGVQIQTLA 

       190        200        210        220        230        240 
LSVQARARGL KPGDASWPEA AYNGDYIADI AADFLAGKTV SASDGEPVTA SGNVEDIDSI 

       250        260        270        280        290        300 
RKFAVTYLRN EQDIDLQAFG VKFDRYYLES SLYSDGRVEA AVQSLVGKGK TYESEGALWL 

       310        320        330        340        350        360 
RTTDDGDDKD RVMKKSDGTY TYFVPDVAYH TTKWERGFTK VINVQGSDHH GTIARVRAGL 

       370        380        390        400        410        420 
QGLDMGIPQG YPDYVLHKMV TVMKNGEEVK ISKRAGSYVT VRDLIEWSNG GDETIRGCLD 

       430        440        450        460        470        480 
TGVADWPQHF TRGRDAVRFF LLSRKADTEF VFDVDLALKQ NDENPVYYVQ YAHARICSIF 

       490        500        510        520        530        540 
ESWGGADWEA RLAELAGADL SAVTGPEASA QAQALGRRLA EFPDMLSGAA AELAPHAVAF 

       550        560        570        580        590 
YLRDLAGDFH AFYNADRVLV DDEAVKRARL ALLAATRQVL RNGLAVIGVS APRRM 

« Hide

References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260479 Genomic DNA. Translation: CAJ91311.1.
RefSeqYP_724679.1. NC_008313.1.

3D structure databases

ProteinModelPortalQ0KFB0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381666.H16_A0159.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ91311; CAJ91311; H16_A0159.
GeneID4247114.
KEGGreh:H16_A0159.
PATRIC35229679. VBIRalEut6770_0511.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0018.
HOGENOMHOG000247214.
KOK01887.
OMAPTHEMER.
OrthoDBEOG6JB13C.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-159-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_00123. Arg_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR11956. PTHR11956. 1 hit.
PfamPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSPR01038. TRNASYNTHARG.
SMARTSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsTIGR00456. argS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYR_CUPNH
AccessionPrimary (citable) accession number: Q0KFB0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries