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Q0KF86 (BIOB_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:H16_A0183
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 343343Biotin synthase HAMAP-Rule MF_01694
PRO_0000381572

Sites

Metal binding791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding831Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1231Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1541Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2141Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2861Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0KF86 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 8CEB639E86FBE3FE

FASTA34337,300
        10         20         30         40         50         60 
MTQAHTATTV TTVSVDSLRQ SARSHALPDD AAWRVDDVAA LFALPFNDLL FRAQQVHREH 

        70         80         90        100        110        120 
FDANTVQLST LLSIKTGGCE EDCGYCPQSA HHDAGVKAEK LMALDEVLDA ARAAKANGAT 

       130        140        150        160        170        180 
RFCMGAAWRS PKDRHLEPVM DMVREVKAMG LETCVTLGML KAEQAQQLKE AGLDYYNHNL 

       190        200        210        220        230        240 
DTSPEFYGKI ITTRTYQDRL DTIGHVRDAG INVCCGGIVG MGEAREARAG LIAQLANMDP 

       250        260        270        280        290        300 
YPESVPINNL VQVEGTPLAG TEALDPFEFV RTIAVARITM PRAMVRLSAG REAMDEALQA 

       310        320        330        340 
LCFMAGANSI FYGEKLLTTG NPQADRDRAL LARLDIRAEG YAG 

« Hide

References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM260479 Genomic DNA. Translation: CAJ91335.1.
RefSeqYP_724703.1. NC_008313.1.

3D structure databases

ProteinModelPortalQ0KF86.
SMRQ0KF86. Positions 33-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381666.H16_A0183.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ91335; CAJ91335; H16_A0183.
GeneID4248811.
KEGGreh:H16_A0183.
PATRIC35229733. VBIRalEut6770_0538.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2309864.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-183-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_CUPNH
AccessionPrimary (citable) accession number: Q0KF86
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 3, 2006
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways