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Q0KF42

- DAPF_CUPNH

UniProt

Q0KF42 - DAPF_CUPNH

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Protein
Diaminopimelate epimerase
Gene
dapF, H16_A0227
Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131Substrate By similarity
Binding sitei46 – 461Substrate By similarity
Binding sitei66 – 661Substrate By similarity
Active sitei75 – 751Proton donor/acceptor By similarity
Binding sitei166 – 1661Substrate By similarity
Sitei168 – 1681Important for catalytic activity By similarity
Binding sitei199 – 1991Substrate By similarity
Sitei217 – 2171Important for catalytic activity By similarity
Active sitei226 – 2261Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-227-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:H16_A0227
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000008210: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Diaminopimelate epimeraseUniRule annotation
PRO_1000011941Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 226UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi381666.H16_A0227.

Structurei

3D structure databases

ProteinModelPortaliQ0KF42.
SMRiQ0KF42. Positions 3-283.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni75 – 773Substrate binding By similarity
Regioni217 – 2182Substrate binding By similarity
Regioni227 – 2282Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220466.
KOiK01778.
OMAiKDITVMV.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0KF42-1 [UniParc]FASTAAdd to Basket

« Hide

MKLQFTKMHG AGNDFVVLDG IHQQIDLTPA QWRALASRHF GVGADQILIV    50
EKPSRPDVDF RYRIVNADGS EVEHCGNGAR CFVRFVTDKG MTDKRSVRVE 100
VMNGVITLTL QDDGQVTVDM GAPELEPARV PFRAEGLPTR AEGADTLYGL 150
EVNGRTEWIS VVSMGNPHAV QVVDDVENFP VLQDGPVVEH HAAFPNRVNA 200
GFMQVQDRHA IRLRVYERGA GETLACGTGA CAAVVAGIRR GLLDSPVRVH 250
THGGDLTIAW DGGAEPVRMT GPATTVFEGS IDLAALPA 288
Length:288
Mass (Da):31,081
Last modified:October 3, 2006 - v1
Checksum:i6451603A073D8C8B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM260479 Genomic DNA. Translation: CAJ91379.1.
RefSeqiYP_724747.1. NC_008313.1.

Genome annotation databases

EnsemblBacteriaiCAJ91379; CAJ91379; H16_A0227.
GeneIDi4246927.
KEGGireh:H16_A0227.
PATRICi35229829. VBIRalEut6770_0586.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM260479 Genomic DNA. Translation: CAJ91379.1 .
RefSeqi YP_724747.1. NC_008313.1.

3D structure databases

ProteinModelPortali Q0KF42.
SMRi Q0KF42. Positions 3-283.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 381666.H16_A0227.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ91379 ; CAJ91379 ; H16_A0227 .
GeneIDi 4246927.
KEGGi reh:H16_A0227.
PATRICi 35229829. VBIRalEut6770_0586.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220466.
KOi K01778.
OMAi KDITVMV.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci CNEC381666:GJUJ-227-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Entry informationi

Entry nameiDAPF_CUPNH
AccessioniPrimary (citable) accession number: Q0KF42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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