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Q0KEU7 (Q0KEU7_CUPNH) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione synthetase HAMAP-Rule MF_00162
EC=6.3.2.3 HAMAP-Rule MF_00162
Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162
Gene names
Name:gshB HAMAP-Rule MF_00162 EMBL CAJ91474.1
Ordered Locus Names:H16_A0323 EMBL CAJ91474.1
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP] EMBL CAJ91474.1
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus ›

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds (magnesium or manganese,ion) per subunit By similarity. HAMAP-Rule MF_00162

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain124 – 309186ATP-grasp By similarity HAMAP-Rule MF_00162
Nucleotide binding150 – 20657ATP By similarity HAMAP-Rule MF_00162

Sites

Metal binding2801Magnesium or manganese By similarity HAMAP-Rule MF_00162
Metal binding2821Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

Sequence LengthMass (Da)Tools
Q0KEU7 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 4644447BA7993F44

FASTA31734,977
        10         20         30         40         50         60 
MRILFITDPL ETFKTYKDST YAMMTEAAAR GHELYWCLQP QLTLSGRVVE TMATRLHLTG 

        70         80         90        100        110        120 
DDHAWYREGN SALEPLSAFD AVLMRKDPPF DMEYVTSTWL LELAEAQGAR VFNKPRAIRD 

       130        140        150        160        170        180 
HSEKLAIAQY PEFITPTLVT RDLARIRDFH AEHRDIIVKP LDGMGGMGVF RVGADGMNLA 

       190        200        210        220        230        240 
AIVETLGHDG ARTLMVQRYI PAIKDGDKRI LLIGGVPVPY SLARVPMAGE VRGNLAAGGT 

       250        260        270        280        290        300 
GRAQELSPRD REIAEALAPG LWEQGLLLVG LDVIGDYLTE VNVTSPTCFQ EITQQTGFHV 

       310 
AAMFIDALEH AVDAAGR

« Hide

References

[1]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM260479 Genomic DNA. Translation: CAJ91474.1.
RefSeqYP_724842.1. NC_008313.1.

3D structure databases

ProteinModelPortalQ0KEU7.
ModBaseSearch...

Protein-protein interaction databases

STRING381666.H16_A0323.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ91474; CAJ91474; H16_A0323.
GeneID4248379.
KEGGreh:H16_A0323.
PATRIC35230031. VBIRalEut6770_0686.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAWMRKDPP.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycCNEC381666:GJUJ-323-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ0KEU7_CUPNH
AccessionPrimary (citable) accession number: Q0KEU7
Entry history
Integrated into UniProtKB/TrEMBL: October 3, 2006
Last sequence update: October 3, 2006
Last modified: May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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