Q0KEQ0 (PTH_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidyl-tRNA hydrolase
Short name=PTH
EC=3.1.1.29

Gene names

Name:	pth
Ordered Locus Names:	H16_A0370

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity. HAMAP MF_00083
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA. HAMAP MF_00083
Subunit structure	Monomer By similarity. HAMAP MF_00083
Subcellular location	Cytoplasm By similarity HAMAP MF_00083.
Sequence similarities	Belongs to the PTH family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Peptidyl-tRNA hydrolase HAMAP MF_00083		PRO_1000010636

Sequences

	Sequence		Length	Mass (Da)
	Q0KEQ0 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 954FFF07761F700E	FASTA	198	21,548
	10 20 30 40 50 60 MIKLIVGLGN PGAEYEATRH NAGFWLVDQL ARMGGATMRV EGRFHGLAAR ARLWDQDIWL 70 80 90 100 110 120 LKPSTFMNRS GLAVVSLARF YKVLPDEIVV AHDEMDLPAG AAKLKRGGGA GGHNGLKDIS 130 140 150 160 170 180 AHLSTQDYWR LRLGVGHPRN APGGAGAGRE DVVNFVLKPP RREEQEAIDA AIDRCIEPLG 190 LLARGDAERA MAQLHTTR

References

[1]

"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM260479 Genomic DNA. Translation: CAJ91521.1.
RefSeq	YP_724889.1. NC_008313.1.
3D structure databases
ProteinModelPortal	Q0KEQ0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0KEQ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4249632.
GenomeReviews	Gene locus H16_A0370 in contig AM260479_GR.
KEGG	reh:H16_A0370.
PATRIC	35230127. VBIRalEut6770_0734.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0193.
HOGENOM	HBG610927.
OMA	IKFKTGG.
PhylomeDB	Q0KEQ0.
ProtClustDB	PRK05426.
Enzyme and pathway databases
BioCyc	REUT381666:H16_A0370-MONOMER.
Family and domain databases
HAMAP	MF_00083. Pept_tRNA_hydro_bact. [Tree]
InterPro	IPR001328. Pept_tRNA_hydro. IPR018171. Pept_tRNA_hydro_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.1470. Pept_tRNA_hydro. 1 hit.
KO	K01056.
PANTHER	PTHR17224. Pept_tRNA_hydro. 1 hit.
Pfam	PF01195. Pept_tRNA_hydro. 1 hit. [Graphical view]
SUPFAM	SSF53178. Pept_tRNA_hydro. 1 hit.
TIGRFAMs	TIGR00447. Pth. 1 hit.
PROSITE	PS01195. PEPT_TRNA_HYDROL_1. 1 hit. PS01196. PEPT_TRNA_HYDROL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTH_CUPNH

Accession

Primary (citable) accession number: Q0KEQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program